Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats
<i>Phlomoides rotata</i>, a traditional medicinal plant, always grows on the Tibetan Plateau at a high altitude of 3100–5200 m. The major active ingredients in <i>P. rotata</i> were used in medicines due to their diverse pharmacological effects, including hemostatic, anti-inf...
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2025-02-01
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| author | Zuxia Li Huichun Xie Guigong Geng Chongxin Yin Xiaozhuo Wu Jianxia Ma Rui Su Zirui Wang Feng Qiao |
| author_facet | Zuxia Li Huichun Xie Guigong Geng Chongxin Yin Xiaozhuo Wu Jianxia Ma Rui Su Zirui Wang Feng Qiao |
| author_sort | Zuxia Li |
| collection | DOAJ |
| description | <i>Phlomoides rotata</i>, a traditional medicinal plant, always grows on the Tibetan Plateau at a high altitude of 3100–5200 m. The major active ingredients in <i>P. rotata</i> were used in medicines due to their diverse pharmacological effects, including hemostatic, anti-inflammatory, antitumor, immuno-modulatory, and antioxidant activities. This study screened 15 top endophytic genus through the analysis of OTUs and the top 30 metabolites with relatively high content in <i>P. rotata</i> roots from four different habitats (HN, GL, YS, and CD regions) in Qinghai Province. Twelve physicochemical indicators were measured and analyzed in the rhizosphere soils of <i>P. rotata</i> habitats. The results indicated that the top 30 metabolites compounds included 7 amino acids, 5 sugars and alcohols, 4 phenylpropanoids, 3 Organic acids, and 3 Alkaloids. Four endophytic bacteria (<i>Acidibacter</i>, <i>Sphingomonas, Variovorax,</i> and <i>Sphingobium</i>) and three endophytic fungi (<i>Tetracladium, Cadophora,</i> and <i>Minimelanolocus</i>) were dominant genera in <i>P. rotata</i> roots from four habitats. There were 109 positive significant correlations and 57 negative correlations between OTUs of endophytic bacteria and contents of top 30 metabolites, and 59 positive significant correlations and 58 negative correlations between OTUs of endophytic fungus and contents of top 30 metabolites. The OTUs of <i>Acidibacter</i> were significantly positively correlated with the content of 5 soil physicochemical indicators (total phosphorus, amylase, sucrase, total potassium, or soil organic carbon) and significantly negatively correlated with the content of acid protease. OTUs of <i>Tetracladium</i> or <i>Cadophora</i> showed a positive correlation with the content of total phosphorus and a negative correlation with that of alkaline phosphatase. This study provides a theoretical basis for the study of the correlation between endophytes and metabolites in <i>P. rotata</i> roots. |
| format | Article |
| id | doaj-art-44a7779224ce4f1db9d76f6fd8732207 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-44a7779224ce4f1db9d76f6fd87322072025-08-20T03:43:14ZengMDPI AGMicroorganisms2076-26072025-02-0113350310.3390/microorganisms13030503Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude HabitatsZuxia Li0Huichun Xie1Guigong Geng2Chongxin Yin3Xiaozhuo Wu4Jianxia Ma5Rui Su6Zirui Wang7Feng Qiao8Key Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaQinghai South of Qilian Mountain Forest Ecosystem Observation and Research Station, Huzhu 810500, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, China<i>Phlomoides rotata</i>, a traditional medicinal plant, always grows on the Tibetan Plateau at a high altitude of 3100–5200 m. The major active ingredients in <i>P. rotata</i> were used in medicines due to their diverse pharmacological effects, including hemostatic, anti-inflammatory, antitumor, immuno-modulatory, and antioxidant activities. This study screened 15 top endophytic genus through the analysis of OTUs and the top 30 metabolites with relatively high content in <i>P. rotata</i> roots from four different habitats (HN, GL, YS, and CD regions) in Qinghai Province. Twelve physicochemical indicators were measured and analyzed in the rhizosphere soils of <i>P. rotata</i> habitats. The results indicated that the top 30 metabolites compounds included 7 amino acids, 5 sugars and alcohols, 4 phenylpropanoids, 3 Organic acids, and 3 Alkaloids. Four endophytic bacteria (<i>Acidibacter</i>, <i>Sphingomonas, Variovorax,</i> and <i>Sphingobium</i>) and three endophytic fungi (<i>Tetracladium, Cadophora,</i> and <i>Minimelanolocus</i>) were dominant genera in <i>P. rotata</i> roots from four habitats. There were 109 positive significant correlations and 57 negative correlations between OTUs of endophytic bacteria and contents of top 30 metabolites, and 59 positive significant correlations and 58 negative correlations between OTUs of endophytic fungus and contents of top 30 metabolites. The OTUs of <i>Acidibacter</i> were significantly positively correlated with the content of 5 soil physicochemical indicators (total phosphorus, amylase, sucrase, total potassium, or soil organic carbon) and significantly negatively correlated with the content of acid protease. OTUs of <i>Tetracladium</i> or <i>Cadophora</i> showed a positive correlation with the content of total phosphorus and a negative correlation with that of alkaline phosphatase. This study provides a theoretical basis for the study of the correlation between endophytes and metabolites in <i>P. rotata</i> roots.https://www.mdpi.com/2076-2607/13/3/503<i>Phlomoides rotata</i>endophytessoil physicochemical indicatorscorrelation analysis |
| spellingShingle | Zuxia Li Huichun Xie Guigong Geng Chongxin Yin Xiaozhuo Wu Jianxia Ma Rui Su Zirui Wang Feng Qiao Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats Microorganisms <i>Phlomoides rotata</i> endophytes soil physicochemical indicators correlation analysis |
| title | Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats |
| title_full | Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats |
| title_fullStr | Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats |
| title_full_unstemmed | Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats |
| title_short | Diversity and Correlation Analysis of Endophytes and Top Metabolites in <i>Phlomoides rotata</i> Roots from High-Altitude Habitats |
| title_sort | diversity and correlation analysis of endophytes and top metabolites in i phlomoides rotata i roots from high altitude habitats |
| topic | <i>Phlomoides rotata</i> endophytes soil physicochemical indicators correlation analysis |
| url | https://www.mdpi.com/2076-2607/13/3/503 |
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