Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells

Interactions between DOM and microbial communities in produced water (PW) from shale gas wells is still lacking. This work examined PW from 15 wells in China's first shale gas field using high-throughput microbial analysis and ultra-high resolution DOM compositional analysis to determine microb...

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Main Authors: Hassan Ramadan, Yufei Ji, Ling Wang, Qiyue Kuang, Zhaoji Zhang
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Desalination and Water Treatment
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Online Access:http://www.sciencedirect.com/science/article/pii/S1944398625002164
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author Hassan Ramadan
Yufei Ji
Ling Wang
Qiyue Kuang
Zhaoji Zhang
author_facet Hassan Ramadan
Yufei Ji
Ling Wang
Qiyue Kuang
Zhaoji Zhang
author_sort Hassan Ramadan
collection DOAJ
description Interactions between DOM and microbial communities in produced water (PW) from shale gas wells is still lacking. This work examined PW from 15 wells in China's first shale gas field using high-throughput microbial analysis and ultra-high resolution DOM compositional analysis to determine microbial diversity, DOM chemodiversity, and their interaction. Results showed the instability of most organic compounds, the composition of the detected semi-volatile organic compounds varied significantly among the samples, and the strongly polar organic compounds were aliphatic. PW samples had higher H/Cwa, lower O/Cwa, and lower double bond equivalent values than river organic matter, indicating more saturated, less polarized, and fewer relative oxygenated compounds. The similarity in CHOS composition in most PW samples was probably due to the addition of similar sulfur-containing chemicals during extraction. The microbial diversity in PW was not rich compared with that in other samples, such as soil and activated sludge. Despite limited microbial diversity, aerobic, microaerophilic and anaerobic bacteria and methanogenic archaea coexisted. Correlation analysis revealed Euryarchaeota could be involved in the degradation of sulfur-containing organics and the accumulation of aromatic compounds. This correlation analysis can provide an in-depth understanding of the transformation of DOM and its interactions with microorganisms in shale gas PW.
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series Desalination and Water Treatment
spelling doaj-art-4495085fac9b495ca48189d140eea5a32025-08-20T03:24:32ZengElsevierDesalination and Water Treatment1944-39862025-04-0132210120010.1016/j.dwt.2025.101200Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wellsHassan Ramadan0Yufei Ji1Ling Wang2Qiyue Kuang3Zhaoji Zhang4Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Public Works Engineering, Faculty of Engineering, Tanta University, Tanta 31511, EgyptInstitute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaFuling District Center for Disease Control and Prevention of Chongqing, Chongqing 408102, China; Corresponding authors.Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaInstitute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Corresponding authors.Interactions between DOM and microbial communities in produced water (PW) from shale gas wells is still lacking. This work examined PW from 15 wells in China's first shale gas field using high-throughput microbial analysis and ultra-high resolution DOM compositional analysis to determine microbial diversity, DOM chemodiversity, and their interaction. Results showed the instability of most organic compounds, the composition of the detected semi-volatile organic compounds varied significantly among the samples, and the strongly polar organic compounds were aliphatic. PW samples had higher H/Cwa, lower O/Cwa, and lower double bond equivalent values than river organic matter, indicating more saturated, less polarized, and fewer relative oxygenated compounds. The similarity in CHOS composition in most PW samples was probably due to the addition of similar sulfur-containing chemicals during extraction. The microbial diversity in PW was not rich compared with that in other samples, such as soil and activated sludge. Despite limited microbial diversity, aerobic, microaerophilic and anaerobic bacteria and methanogenic archaea coexisted. Correlation analysis revealed Euryarchaeota could be involved in the degradation of sulfur-containing organics and the accumulation of aromatic compounds. This correlation analysis can provide an in-depth understanding of the transformation of DOM and its interactions with microorganisms in shale gas PW.http://www.sciencedirect.com/science/article/pii/S1944398625002164Shale gas produced waterDissolved organic matterChemodiversityMethanogensCorrelation analysis
spellingShingle Hassan Ramadan
Yufei Ji
Ling Wang
Qiyue Kuang
Zhaoji Zhang
Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
Desalination and Water Treatment
Shale gas produced water
Dissolved organic matter
Chemodiversity
Methanogens
Correlation analysis
title Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
title_full Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
title_fullStr Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
title_full_unstemmed Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
title_short Chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
title_sort chemodiversity of dissolved organic matter in produced water correlates with the microbial community in fifteen shale gas wells
topic Shale gas produced water
Dissolved organic matter
Chemodiversity
Methanogens
Correlation analysis
url http://www.sciencedirect.com/science/article/pii/S1944398625002164
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AT lingwang chemodiversityofdissolvedorganicmatterinproducedwatercorrelateswiththemicrobialcommunityinfifteenshalegaswells
AT qiyuekuang chemodiversityofdissolvedorganicmatterinproducedwatercorrelateswiththemicrobialcommunityinfifteenshalegaswells
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