Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation
The aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2013/629543 |
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| author | Elena A. Shubina Mikhail A. Nikitin Ekaterina V. Ponomareva Denis V. Goryunov Oleg F. Gritsenko |
| author_facet | Elena A. Shubina Mikhail A. Nikitin Ekaterina V. Ponomareva Denis V. Goryunov Oleg F. Gritsenko |
| author_sort | Elena A. Shubina |
| collection | DOAJ |
| description | The aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera) and the Coregonidae families under different levels of reproductive isolation. Two basic approaches were used: (1) PCR-RAPD with a 20–22 nt primer design with subsequent cloning and sequencing of the products and (2) a modified endonuclease restriction analysis. The restriction fragments were shown with sequencing to represent satellite DNA. Effects of speciation are found in repetitive sequences. The revelation of expressed sequences in the majority of the employed anonymous loci allows for assuming the adaptive selection during allopatric speciation in isolated char forms. |
| format | Article |
| id | doaj-art-4493dcf4da9e4ccc9b6c2eae02263d6e |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4493dcf4da9e4ccc9b6c2eae02263d6e2025-02-03T05:54:38ZengWileyInternational Journal of Genomics2314-436X2314-43782013-01-01201310.1155/2013/629543629543Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic IsolationElena A. Shubina0Mikhail A. Nikitin1Ekaterina V. Ponomareva2Denis V. Goryunov3Oleg F. Gritsenko4Belozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, RussiaBelozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, RussiaBelozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, RussiaBelozersky Institute for Physico-Chemical Biology of Lomonosov Moscow State University, Leninskie gory, 1, Moscow 119991, RussiaRussian Federative Research Institute of Fisheries and Oceanology, 17A V. Krasnoselskaya Street, Moscow 107140, RussiaThe aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera) and the Coregonidae families under different levels of reproductive isolation. Two basic approaches were used: (1) PCR-RAPD with a 20–22 nt primer design with subsequent cloning and sequencing of the products and (2) a modified endonuclease restriction analysis. The restriction fragments were shown with sequencing to represent satellite DNA. Effects of speciation are found in repetitive sequences. The revelation of expressed sequences in the majority of the employed anonymous loci allows for assuming the adaptive selection during allopatric speciation in isolated char forms.http://dx.doi.org/10.1155/2013/629543 |
| spellingShingle | Elena A. Shubina Mikhail A. Nikitin Ekaterina V. Ponomareva Denis V. Goryunov Oleg F. Gritsenko Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation International Journal of Genomics |
| title | Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation |
| title_full | Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation |
| title_fullStr | Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation |
| title_full_unstemmed | Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation |
| title_short | Comparative Study of Genome Divergence in Salmonids with Various Rates of Genetic Isolation |
| title_sort | comparative study of genome divergence in salmonids with various rates of genetic isolation |
| url | http://dx.doi.org/10.1155/2013/629543 |
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