Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status
In the current study, we tested the effects of dietary cowpea protein hydrolysate (CPH) on broilers’ growth, carcase traits, intestinal histomorphology, The fatty acid profile of breast muscle, blood biochemical parameters, and immune status. Three-day age male Ross-308 broilers (n = 300, average we...
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2274508 |
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| author | Samar A. Tolba Shimaa A. Amer Ahmed Gouda Ali Osman Wafaa R. I. A. Sherief Amany I. Ahmed Ghada I. Abd El-Rahman Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Simon J. Davies Elshimaa M. Roushdy |
| author_facet | Samar A. Tolba Shimaa A. Amer Ahmed Gouda Ali Osman Wafaa R. I. A. Sherief Amany I. Ahmed Ghada I. Abd El-Rahman Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Simon J. Davies Elshimaa M. Roushdy |
| author_sort | Samar A. Tolba |
| collection | DOAJ |
| description | In the current study, we tested the effects of dietary cowpea protein hydrolysate (CPH) on broilers’ growth, carcase traits, intestinal histomorphology, The fatty acid profile of breast muscle, blood biochemical parameters, and immune status. Three-day age male Ross-308 broilers (n = 300, average weight 86.72 g ± 0.20) were distributed into five groups and fed on five diets supplied with five levels of CPH at 0, 2, 4, 6, and 8 g kg−1 for 35 days. The results revealed that dietary CPH did not change (P < 0.05) the broilers’ growth performance or carcase traits, except for a quadratic decrease (P < 0.05) in FCR at concentrations 2 and 4 g kg−1 during the finisher period. The villus heights (VH) and goblet cell count (GCC) were increased (P < 0.05) in the duodenum and jejunum; however, the villus width decreased (P < 0.05) in the duodenum without showing changes (P < 0.05) in the jejunum and ileum. Dietary supplementation with 6 g CPH kg−1 increased (P < 0.05) the duodenal crypt depth (CD). Dietary supplementation with 8 g CPH kg −1 increased the VH: CD in the duodenum compared to the control; nevertheless, a quadratic (P < 0.05) change was reported in the jejunum. The VH: CD did not change (P < 0.05) in the ileum; however, dietary supplementation with 6 CPH kg−1 increased (P < 0.05) the ileal VH and GCC. The inclusion of CPH led to a linear (P < 0.05) increase in the total n-3 polyunsaturated fatty acids (PUFA), n-6 PUFA, and n-3/n-6 PUFA in broilers’ breast muscle. An increase in the assessed metabolic and immune status indices was reported, as evidenced by increases (P < 0.05) in thyroid hormones, growth hormone, total protein, albumin, globulin, lysozymes, interferon-gamma, interleukin10, and complement 3 serum levels. However, the serum glucose and leptin levels did not show significant (p > 0.05) changes in response to dietary CPH. Moreover, the dietary CPH levels 4–8 g kg−1 upregulated the immunostaining of IgG in the spleen of these groups. These findings suggested that including CPH in broiler diets could be an effective candidate for modulating the chickens’ metabolic and immune status; however, it had no increased effect on broiler growth. |
| format | Article |
| id | doaj-art-448ff9af5ca0401fa0c76107330c33e4 |
| institution | Kabale University |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-448ff9af5ca0401fa0c76107330c33e42025-08-20T03:31:11ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-012211204121810.1080/1828051X.2023.22745082274508Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune statusSamar A. Tolba0Shimaa A. Amer1Ahmed Gouda2Ali Osman3Wafaa R. I. A. Sherief4Amany I. Ahmed5Ghada I. Abd El-Rahman6Abdel-Wahab A. Abdel-Warith7Elsayed M. Younis8Simon J. Davies9Elshimaa M. Roushdy10Department of Nutrition and Clinical Nutrition, Zagazig UniversityDepartment of Nutrition and Clinical Nutrition, Zagazig UniversityAnimal Production Department, Agricultural & Biological Research Division, National Research CenterBiochemistry Department, Zagazig UniversityAnimal Wealth Development Department, Zagazig UniversityDepartment of Biochemistry, Zagazig UniversityDepartment of Clinical Pathology, Zagazig UniversityDepartment of Zoology, College of Science,- King Saud UniversityDepartment of Zoology, College of Science,- King Saud UniversityAquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of GalwayAnimal Wealth Development Department, Zagazig UniversityIn the current study, we tested the effects of dietary cowpea protein hydrolysate (CPH) on broilers’ growth, carcase traits, intestinal histomorphology, The fatty acid profile of breast muscle, blood biochemical parameters, and immune status. Three-day age male Ross-308 broilers (n = 300, average weight 86.72 g ± 0.20) were distributed into five groups and fed on five diets supplied with five levels of CPH at 0, 2, 4, 6, and 8 g kg−1 for 35 days. The results revealed that dietary CPH did not change (P < 0.05) the broilers’ growth performance or carcase traits, except for a quadratic decrease (P < 0.05) in FCR at concentrations 2 and 4 g kg−1 during the finisher period. The villus heights (VH) and goblet cell count (GCC) were increased (P < 0.05) in the duodenum and jejunum; however, the villus width decreased (P < 0.05) in the duodenum without showing changes (P < 0.05) in the jejunum and ileum. Dietary supplementation with 6 g CPH kg−1 increased (P < 0.05) the duodenal crypt depth (CD). Dietary supplementation with 8 g CPH kg −1 increased the VH: CD in the duodenum compared to the control; nevertheless, a quadratic (P < 0.05) change was reported in the jejunum. The VH: CD did not change (P < 0.05) in the ileum; however, dietary supplementation with 6 CPH kg−1 increased (P < 0.05) the ileal VH and GCC. The inclusion of CPH led to a linear (P < 0.05) increase in the total n-3 polyunsaturated fatty acids (PUFA), n-6 PUFA, and n-3/n-6 PUFA in broilers’ breast muscle. An increase in the assessed metabolic and immune status indices was reported, as evidenced by increases (P < 0.05) in thyroid hormones, growth hormone, total protein, albumin, globulin, lysozymes, interferon-gamma, interleukin10, and complement 3 serum levels. However, the serum glucose and leptin levels did not show significant (p > 0.05) changes in response to dietary CPH. Moreover, the dietary CPH levels 4–8 g kg−1 upregulated the immunostaining of IgG in the spleen of these groups. These findings suggested that including CPH in broiler diets could be an effective candidate for modulating the chickens’ metabolic and immune status; however, it had no increased effect on broiler growth.http://dx.doi.org/10.1080/1828051X.2023.2274508broiler chickenenzymatic hydrolysisgrowthgut healthimmunohistochemistryprotein hydrolysates |
| spellingShingle | Samar A. Tolba Shimaa A. Amer Ahmed Gouda Ali Osman Wafaa R. I. A. Sherief Amany I. Ahmed Ghada I. Abd El-Rahman Abdel-Wahab A. Abdel-Warith Elsayed M. Younis Simon J. Davies Elshimaa M. Roushdy Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status Italian Journal of Animal Science broiler chicken enzymatic hydrolysis growth gut health immunohistochemistry protein hydrolysates |
| title | Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status |
| title_full | Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status |
| title_fullStr | Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status |
| title_full_unstemmed | Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status |
| title_short | Potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens: effects on growth, intestinal morphology, muscle lipid profile, and immune status |
| title_sort | potential use of cowpea protein hydrolysate as a dietary supplement in broiler chickens effects on growth intestinal morphology muscle lipid profile and immune status |
| topic | broiler chicken enzymatic hydrolysis growth gut health immunohistochemistry protein hydrolysates |
| url | http://dx.doi.org/10.1080/1828051X.2023.2274508 |
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