Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites

Indoplanorbis exustus and Radix rubiginosa act as intermediate hosts for veterinary and medical trematode parasites. Snail control is a strategy used to decrease the number of snails and interrupt the life cycle of parasites. The objective of this study was to evaluate the efficacy of Xenorhabdus an...

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Main Authors: Abdulhakam Dumidae, Chanatinart Homkeaw, Chanakan Subkrasae, Jiranun Ardpairin, Supawan Pansri, Raxsina Polseela, Ittipon Phoungpetchara, Tewarat Kumchantuek, Sarunporn Tandhavanan, Aunchalee Thanwisai, Apichat Vitta
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Parasite Epidemiology and Control
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405673124000394
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author Abdulhakam Dumidae
Chanatinart Homkeaw
Chanakan Subkrasae
Jiranun Ardpairin
Supawan Pansri
Raxsina Polseela
Ittipon Phoungpetchara
Tewarat Kumchantuek
Sarunporn Tandhavanan
Aunchalee Thanwisai
Apichat Vitta
author_facet Abdulhakam Dumidae
Chanatinart Homkeaw
Chanakan Subkrasae
Jiranun Ardpairin
Supawan Pansri
Raxsina Polseela
Ittipon Phoungpetchara
Tewarat Kumchantuek
Sarunporn Tandhavanan
Aunchalee Thanwisai
Apichat Vitta
author_sort Abdulhakam Dumidae
collection DOAJ
description Indoplanorbis exustus and Radix rubiginosa act as intermediate hosts for veterinary and medical trematode parasites. Snail control is a strategy used to decrease the number of snails and interrupt the life cycle of parasites. The objective of this study was to evaluate the efficacy of Xenorhabdus and Photorhabdus extracts against I. exustus and R. rubiginosa in the laboratory. Ethyl acetate extracts of selected symbiotic bacteria were tested for their molluscicidal activities according to World Health Organization guidelines. Additionally, pathological changes in the snails were observed after treatment with the LC50 values under a light microscope. Indoplanorbis exustus and R. rubiginosa were susceptible to all ethyl acetate extracts of symbiotic bacteria. The lowest LC50 and LC90 at 24 h for I. exustus after exposure to Photorhabdus laumondii subsp. laumondii (bALN18.2_TH) extracts were 81.66 and 151.02 ppm, respectively. Similarly, the lowest LC50 and LC90 at 24 h for R. rubiginosa after exposure to Photorhabdus luminescence subsp. akhurstii (bAPY3.5_TH) extracts were 49.21 and 147.66 ppm, respectively. Photorhabdus species had more substantial molluscicidal effects than Xenorhabdus on these snails. The ethyl acetate extracts of these bacteria are effective when contacting the epithelial cells and foot muscle of the snails. To our knowledge, this is the first report on using Xenorhabdus and Photorhabdus extracts to evaluate molluscicidal activities. These symbiotic bacteria, Xenorhabdus and Photorhabdus, may be useful for controlling snail intermediate hosts.
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spelling doaj-art-448fba2274c44709ae526da7f8b375a02025-08-20T02:36:59ZengElsevierParasite Epidemiology and Control2405-67312024-11-0127e0037510.1016/j.parepi.2024.e00375Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasitesAbdulhakam Dumidae0Chanatinart Homkeaw1Chanakan Subkrasae2Jiranun Ardpairin3Supawan Pansri4Raxsina Polseela5Ittipon Phoungpetchara6Tewarat Kumchantuek7Sarunporn Tandhavanan8Aunchalee Thanwisai9Apichat Vitta10Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence for Biodiversity, Faculty of Sciences, Naresuan University, Phitsanulok 65000, ThailandDepartment of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok, ThailandDepartment of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence for Biodiversity, Faculty of Sciences, Naresuan University, Phitsanulok 65000, ThailandDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence for Biodiversity, Faculty of Sciences, Naresuan University, Phitsanulok 65000, Thailand; Corresponding author at: Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.Indoplanorbis exustus and Radix rubiginosa act as intermediate hosts for veterinary and medical trematode parasites. Snail control is a strategy used to decrease the number of snails and interrupt the life cycle of parasites. The objective of this study was to evaluate the efficacy of Xenorhabdus and Photorhabdus extracts against I. exustus and R. rubiginosa in the laboratory. Ethyl acetate extracts of selected symbiotic bacteria were tested for their molluscicidal activities according to World Health Organization guidelines. Additionally, pathological changes in the snails were observed after treatment with the LC50 values under a light microscope. Indoplanorbis exustus and R. rubiginosa were susceptible to all ethyl acetate extracts of symbiotic bacteria. The lowest LC50 and LC90 at 24 h for I. exustus after exposure to Photorhabdus laumondii subsp. laumondii (bALN18.2_TH) extracts were 81.66 and 151.02 ppm, respectively. Similarly, the lowest LC50 and LC90 at 24 h for R. rubiginosa after exposure to Photorhabdus luminescence subsp. akhurstii (bAPY3.5_TH) extracts were 49.21 and 147.66 ppm, respectively. Photorhabdus species had more substantial molluscicidal effects than Xenorhabdus on these snails. The ethyl acetate extracts of these bacteria are effective when contacting the epithelial cells and foot muscle of the snails. To our knowledge, this is the first report on using Xenorhabdus and Photorhabdus extracts to evaluate molluscicidal activities. These symbiotic bacteria, Xenorhabdus and Photorhabdus, may be useful for controlling snail intermediate hosts.http://www.sciencedirect.com/science/article/pii/S2405673124000394Biological controlEntomopathogenic nematodesIntermediate hostPhotorhabdusXenorhabdus
spellingShingle Abdulhakam Dumidae
Chanatinart Homkeaw
Chanakan Subkrasae
Jiranun Ardpairin
Supawan Pansri
Raxsina Polseela
Ittipon Phoungpetchara
Tewarat Kumchantuek
Sarunporn Tandhavanan
Aunchalee Thanwisai
Apichat Vitta
Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
Parasite Epidemiology and Control
Biological control
Entomopathogenic nematodes
Intermediate host
Photorhabdus
Xenorhabdus
title Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
title_full Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
title_fullStr Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
title_full_unstemmed Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
title_short Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
title_sort molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against indoplanorbis exustus and radix rubiginosa the intermediate hosts of trematode parasites
topic Biological control
Entomopathogenic nematodes
Intermediate host
Photorhabdus
Xenorhabdus
url http://www.sciencedirect.com/science/article/pii/S2405673124000394
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