Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model

Background: High-fat diets habit can lead to metabolic disorders, such as dyslipidemia which a significant contributor for cardiovascular diseases. Dyslipidemia occurs as a result of metabolically interrelated abnormalities of plasma lipids and lipoproteins, including low level of high-density lipop...

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Main Authors: Yulia Rohman, Arief Nurudhin, Lusi Oka Wardhani
Format: Article
Language:Indonesian
Published: Diponegoro University 2024-06-01
Series:Jurnal Gizi Indonesia: The Indonesian Journal of Nutrition
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Online Access:https://ejournal.undip.ac.id/index.php/jgi/article/view/58198
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author Yulia Rohman
Arief Nurudhin
Lusi Oka Wardhani
author_facet Yulia Rohman
Arief Nurudhin
Lusi Oka Wardhani
author_sort Yulia Rohman
collection DOAJ
description Background: High-fat diets habit can lead to metabolic disorders, such as dyslipidemia which a significant contributor for cardiovascular diseases. Dyslipidemia occurs as a result of metabolically interrelated abnormalities of plasma lipids and lipoproteins, including low level of high-density lipoprotein cholesterol, and increasing of low density lipoprotein cholesterol, total cholesterol, and triglyceride. In order to enhance anti-lipid treatment, nutritional therapy containing bioactive compounds are investigated extensively, including those found in beetroot which shown potential in preventing and treating metabolic disorders. Objective: This study evaluated the effect of beetroot extract and beetroot juice on cholesterol and triglyceride levels as a dyslipidemia treatment, in comparison to simvastatin. Ethanol extract can attract flavonoids and betalain, but the extraction process can degrade fiber. Meanwhile, juice still contains quite a lot of fiber. Materials and Methods: Male Sprague Dawley rats were divided into seven groups and fed different diets for 56 days. The groups were: normal control (K0), negative control-HFD (KN), positive control-HFD + simvastatin (KP), single-dose treatment with BE (P1) or BJ (P2), and combination treatment with BE or BJ + simvastatin (P3 and P4), each intervention was given for 28 days. After blood drawn, cholesterol total and triglyceride serum were examined and analyzed. Results: Administration of beetroot extract and juice in single dose or combination with simvastatin gave a significant decrease in cholesterol and triglyceride levels compared to before the intervention. The average reduction levels of cholesterol in P1, P2, P3, and P4 were 54.81, 56.31, 94.19 and 69.11 respectively. Whereas the average decreasing level of triglyceride were 43.28, 30.78, 54.28 and 46.37 in P1, P2, P3 and P4 groups. Combination treatment with simvastatin gave more reduction level compared with single dose beetroot extract or beetroot juice. The most effective reduction was in the beet extract combination with simvastatin group were -94.19±4.08 mg/dL (cholesterol) and -54.28±6.93 mg/dL (triglyceride). Conclusion: Both beetroot extract and juice, as single or combined with simvastatin, were able to decrease cholesterol and triglyceride levels, these indicated their potential for prevention and therapeutic in dyslipidemia. Further research is needed to investigate its mechanisms and establish optimal dosages for human consumption.
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issn 2338-3119
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publishDate 2024-06-01
publisher Diponegoro University
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series Jurnal Gizi Indonesia: The Indonesian Journal of Nutrition
spelling doaj-art-448a6a6af77f42c280538bb9e87be2a02025-08-20T02:40:33ZindDiponegoro UniversityJurnal Gizi Indonesia: The Indonesian Journal of Nutrition2338-31192024-06-01122808710.14710/jgi.12.2.80-8724180Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley modelYulia Rohman0https://orcid.org/0000-0002-1542-686XArief Nurudhin1Lusi Oka Wardhani2https://orcid.org/0000-0002-9895-9038Department of Clinical Nutrition, Postgraduate Program, Universitas Sebelas Maret, Surakarta, Jawa Tengah, IndonesiaInternal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, IndonesiaClinical Pathology Department, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, IndonesiaBackground: High-fat diets habit can lead to metabolic disorders, such as dyslipidemia which a significant contributor for cardiovascular diseases. Dyslipidemia occurs as a result of metabolically interrelated abnormalities of plasma lipids and lipoproteins, including low level of high-density lipoprotein cholesterol, and increasing of low density lipoprotein cholesterol, total cholesterol, and triglyceride. In order to enhance anti-lipid treatment, nutritional therapy containing bioactive compounds are investigated extensively, including those found in beetroot which shown potential in preventing and treating metabolic disorders. Objective: This study evaluated the effect of beetroot extract and beetroot juice on cholesterol and triglyceride levels as a dyslipidemia treatment, in comparison to simvastatin. Ethanol extract can attract flavonoids and betalain, but the extraction process can degrade fiber. Meanwhile, juice still contains quite a lot of fiber. Materials and Methods: Male Sprague Dawley rats were divided into seven groups and fed different diets for 56 days. The groups were: normal control (K0), negative control-HFD (KN), positive control-HFD + simvastatin (KP), single-dose treatment with BE (P1) or BJ (P2), and combination treatment with BE or BJ + simvastatin (P3 and P4), each intervention was given for 28 days. After blood drawn, cholesterol total and triglyceride serum were examined and analyzed. Results: Administration of beetroot extract and juice in single dose or combination with simvastatin gave a significant decrease in cholesterol and triglyceride levels compared to before the intervention. The average reduction levels of cholesterol in P1, P2, P3, and P4 were 54.81, 56.31, 94.19 and 69.11 respectively. Whereas the average decreasing level of triglyceride were 43.28, 30.78, 54.28 and 46.37 in P1, P2, P3 and P4 groups. Combination treatment with simvastatin gave more reduction level compared with single dose beetroot extract or beetroot juice. The most effective reduction was in the beet extract combination with simvastatin group were -94.19±4.08 mg/dL (cholesterol) and -54.28±6.93 mg/dL (triglyceride). Conclusion: Both beetroot extract and juice, as single or combined with simvastatin, were able to decrease cholesterol and triglyceride levels, these indicated their potential for prevention and therapeutic in dyslipidemia. Further research is needed to investigate its mechanisms and establish optimal dosages for human consumption.https://ejournal.undip.ac.id/index.php/jgi/article/view/58198dyslipidemiabeetroothigh fat dietcholesteroltriglyceride
spellingShingle Yulia Rohman
Arief Nurudhin
Lusi Oka Wardhani
Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
Jurnal Gizi Indonesia: The Indonesian Journal of Nutrition
dyslipidemia
beetroot
high fat diet
cholesterol
triglyceride
title Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
title_full Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
title_fullStr Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
title_full_unstemmed Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
title_short Beetroot (Beta vulgaris L.) reduces cholesterol and triglyceride in dyslipidemic male rats sprague–dawley model
title_sort beetroot beta vulgaris l reduces cholesterol and triglyceride in dyslipidemic male rats sprague dawley model
topic dyslipidemia
beetroot
high fat diet
cholesterol
triglyceride
url https://ejournal.undip.ac.id/index.php/jgi/article/view/58198
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AT ariefnurudhin beetrootbetavulgarislreducescholesterolandtriglycerideindyslipidemicmaleratsspraguedawleymodel
AT lusiokawardhani beetrootbetavulgarislreducescholesterolandtriglycerideindyslipidemicmaleratsspraguedawleymodel