Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model
Background and objectives: Nanofibers have a high specific surface area and small pores, which increases the possibility of drug uptake. In this study, chasteberry-containing nanofibers were prepared by electrospinning to investigate their potential for polycystic follicles after induction of polycy...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137024001341 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591762263113728 |
|---|---|
| author | Zaid H. Mahmoud Uday Abdul-Reda Hussein Najwa Aljbory Mohammed Jawad Alnajar Laleh Maleknia Abolfazl Mirani Ehsan kianfar |
| author_facet | Zaid H. Mahmoud Uday Abdul-Reda Hussein Najwa Aljbory Mohammed Jawad Alnajar Laleh Maleknia Abolfazl Mirani Ehsan kianfar |
| author_sort | Zaid H. Mahmoud |
| collection | DOAJ |
| description | Background and objectives: Nanofibers have a high specific surface area and small pores, which increases the possibility of drug uptake. In this study, chasteberry-containing nanofibers were prepared by electrospinning to investigate their potential for polycystic follicles after induction of polycystic ovarian syndrome (PCOS). Materials and methods: Chasteberry-containing nanofibers were identified using Fourier-transform infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), and thermal gravimetric analysis (TGA) tests, as well as drug release and antibacterial tests. In this study, 42 Sprague-Dawley rats with regular menstrual cycles were divided into five groups: control group (healthy rats), polycarbonate (PC) group (treated with 1 mg/kg letrozole for 28 days to induce PCOS syndrome), and Poly terephthalate (PT) group (treated with PCOS for 30 days). Chasteberry was included at 1, 2, 4 wt%. To induce PCOS, the polyterephthalate (PT) and polycarbonate (PC) groups were administered 1 mg/kg letrozole by oral gavage for 28 days. The polyterephthalate (PT) group was treated with different doses of chasteberry-containing nanofibers (1, 2, 4 wt%). Results: The results showed that the drug was well incorporated into the fibers and released slowly and sustainedly from the nanofibers for 30 days. Ex vivo results showed that by taking letrozole, the polycarbonate (PC) group had larger cystic follicles with a very thin granulosa layer than the control group. This is the cause of ovarian anovulation or oligo-ovulation and induction of polycystic ovarian syndrome. In the polyterephthalate (PT) group, which was administered nanofibers containing chasteberry nanomedicine subcutaneously, the number of cystic follicles decreased and the number of various follicles indicating ovulation increased in these groups. Conclusion: Subcutaneous administration of Vitex nanofibers at a dose of 4 wt% can improve polycystic ovarian syndrome by decreasing cystic follicles and increasing the number of various follicles and corpora lutea. |
| format | Article |
| id | doaj-art-448342ca03b3452a92de71707a4b9011 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-448342ca03b3452a92de71707a4b90112025-01-22T05:43:55ZengElsevierBiosensors and Bioelectronics: X2590-13702025-03-0122100570Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal modelZaid H. Mahmoud0Uday Abdul-Reda Hussein1Najwa Aljbory2Mohammed Jawad Alnajar3Laleh Maleknia4Abolfazl Mirani5Ehsan kianfar6college of Sciences, University of Diyala, chemistry Department, IraqCollege of Pharmacy, University of Al-Ameed, IraqAlSafwa University College, IraqDepartment of Medical Laboratories Technology, AL-Nisour University College, Baghdad, IraqDepartment of Biomedical Engineering, Islamic Azad University, South Tehran Branch, Tehran, Iran; Corresponding author.Biomedical Engineering Research Center, Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; Corresponding author.Young Researchers and Elite Club, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran; Corresponding author.Background and objectives: Nanofibers have a high specific surface area and small pores, which increases the possibility of drug uptake. In this study, chasteberry-containing nanofibers were prepared by electrospinning to investigate their potential for polycystic follicles after induction of polycystic ovarian syndrome (PCOS). Materials and methods: Chasteberry-containing nanofibers were identified using Fourier-transform infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), and thermal gravimetric analysis (TGA) tests, as well as drug release and antibacterial tests. In this study, 42 Sprague-Dawley rats with regular menstrual cycles were divided into five groups: control group (healthy rats), polycarbonate (PC) group (treated with 1 mg/kg letrozole for 28 days to induce PCOS syndrome), and Poly terephthalate (PT) group (treated with PCOS for 30 days). Chasteberry was included at 1, 2, 4 wt%. To induce PCOS, the polyterephthalate (PT) and polycarbonate (PC) groups were administered 1 mg/kg letrozole by oral gavage for 28 days. The polyterephthalate (PT) group was treated with different doses of chasteberry-containing nanofibers (1, 2, 4 wt%). Results: The results showed that the drug was well incorporated into the fibers and released slowly and sustainedly from the nanofibers for 30 days. Ex vivo results showed that by taking letrozole, the polycarbonate (PC) group had larger cystic follicles with a very thin granulosa layer than the control group. This is the cause of ovarian anovulation or oligo-ovulation and induction of polycystic ovarian syndrome. In the polyterephthalate (PT) group, which was administered nanofibers containing chasteberry nanomedicine subcutaneously, the number of cystic follicles decreased and the number of various follicles indicating ovulation increased in these groups. Conclusion: Subcutaneous administration of Vitex nanofibers at a dose of 4 wt% can improve polycystic ovarian syndrome by decreasing cystic follicles and increasing the number of various follicles and corpora lutea.http://www.sciencedirect.com/science/article/pii/S2590137024001341NanofiberPoly-lactic acidVitex plantPolycystic ovary syndromeRat |
| spellingShingle | Zaid H. Mahmoud Uday Abdul-Reda Hussein Najwa Aljbory Mohammed Jawad Alnajar Laleh Maleknia Abolfazl Mirani Ehsan kianfar Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model Biosensors and Bioelectronics: X Nanofiber Poly-lactic acid Vitex plant Polycystic ovary syndrome Rat |
| title | Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| title_full | Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| title_fullStr | Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| title_full_unstemmed | Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| title_short | Development of vitex-derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| title_sort | development of vitex derived polymer nanofibers using electrochemical sensors for the treatment of polycystic ovarian syndrome in rats as an animal model |
| topic | Nanofiber Poly-lactic acid Vitex plant Polycystic ovary syndrome Rat |
| url | http://www.sciencedirect.com/science/article/pii/S2590137024001341 |
| work_keys_str_mv | AT zaidhmahmoud developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT udayabdulredahussein developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT najwaaljbory developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT mohammedjawadalnajar developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT lalehmaleknia developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT abolfazlmirani developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel AT ehsankianfar developmentofvitexderivedpolymernanofibersusingelectrochemicalsensorsforthetreatmentofpolycysticovariansyndromeinratsasananimalmodel |