Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose
Objective To investigate the effects and mechanisms of telmisartan on expression of adiponectin receptors 1/2 in rat renal proximal tubular epithelial cells induced by high glucose.Methods Cultured rat renal proximal tubular epithelial cells( NRK-52 E) which were synchronized in vitro were divided i...
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Editorial Department of Journal of Clinical Nephrology
2019-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| author | BU Xi LIANG Shan-shan ZHA Dong-qing CHEN Pei-na WU Xiao-yan |
| author_facet | BU Xi LIANG Shan-shan ZHA Dong-qing CHEN Pei-na WU Xiao-yan |
| author_sort | BU Xi |
| collection | DOAJ |
| description | Objective To investigate the effects and mechanisms of telmisartan on expression of adiponectin receptors 1/2 in rat renal proximal tubular epithelial cells induced by high glucose.Methods Cultured rat renal proximal tubular epithelial cells( NRK-52 E) which were synchronized in vitro were divided into 6 groups: normal control group( 5. 5 mmol/L glucose medium culture,24 h),hypertonic control group( 5. 5 mmol/L glucose medium with 19. 5 mmol/L mannitol treatment,24 h),high glucose group( 25 mmol/L glucose medium for 12 h,24 h,48 h,72 h respectively),high glucose +telmisartan group [25 mmol/L glucose medium with different concentrations of telmisartan( 1,10,100 nmol/L) for 24 h],high glucose + telmisartan + GW9662 group( pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min,then 25 mmol/L glucose and 100 nmol/L telmisartan for 24 h),high glucose + GW9662 group( pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min,then 25 mmol/L glucose for 24 h). The reverse transcription time-polymerase chain reaction( RT-PCR)and Western blotting were used to detect the expression of adiponectin-receptors 1/2,PPAR-γ mRNA and protein levels in each group. Results In rat renal proximal tubular epithelial cells stimulated by high glucose,telmisartan could promote adiponectin receptors 1/2 expression through the pathway of PPAR-γ. Conclusions In rat renal proximal tubular epithelial cells stimulated by high glucose,telmisartan could promote adiponectin receptor 1/2 expression through the pathway of PPAR-γ. |
| format | Article |
| id | doaj-art-447c85c9a2f248609b286fa1c7d57f10 |
| institution | DOAJ |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2019-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
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| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-447c85c9a2f248609b286fa1c7d57f102025-08-20T03:09:25ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902019-01-01646957914161Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucoseBU XiLIANG Shan-shanZHA Dong-qingCHEN Pei-naWU Xiao-yanObjective To investigate the effects and mechanisms of telmisartan on expression of adiponectin receptors 1/2 in rat renal proximal tubular epithelial cells induced by high glucose.Methods Cultured rat renal proximal tubular epithelial cells( NRK-52 E) which were synchronized in vitro were divided into 6 groups: normal control group( 5. 5 mmol/L glucose medium culture,24 h),hypertonic control group( 5. 5 mmol/L glucose medium with 19. 5 mmol/L mannitol treatment,24 h),high glucose group( 25 mmol/L glucose medium for 12 h,24 h,48 h,72 h respectively),high glucose +telmisartan group [25 mmol/L glucose medium with different concentrations of telmisartan( 1,10,100 nmol/L) for 24 h],high glucose + telmisartan + GW9662 group( pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min,then 25 mmol/L glucose and 100 nmol/L telmisartan for 24 h),high glucose + GW9662 group( pretreatment with 5 000 nmol/L PPARγ blocker GW9662 for 30 min,then 25 mmol/L glucose for 24 h). The reverse transcription time-polymerase chain reaction( RT-PCR)and Western blotting were used to detect the expression of adiponectin-receptors 1/2,PPAR-γ mRNA and protein levels in each group. Results In rat renal proximal tubular epithelial cells stimulated by high glucose,telmisartan could promote adiponectin receptors 1/2 expression through the pathway of PPAR-γ. Conclusions In rat renal proximal tubular epithelial cells stimulated by high glucose,telmisartan could promote adiponectin receptor 1/2 expression through the pathway of PPAR-γ.http://www.lcszb.com/thesisDetails?columnId=57914161&Fpath=home&index=0Adiponectin receptorTelmisartanRat renal tubular epithelial cellsPeroxisome proliferator-activated receptor-γ |
| spellingShingle | BU Xi LIANG Shan-shan ZHA Dong-qing CHEN Pei-na WU Xiao-yan Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose Linchuang shenzangbing zazhi Adiponectin receptor Telmisartan Rat renal tubular epithelial cells Peroxisome proliferator-activated receptor-γ |
| title | Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| title_full | Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| title_fullStr | Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| title_full_unstemmed | Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| title_short | Effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| title_sort | effects and mechanisms of telmisartan on adiponectin receptors in rat renal proximal tubular epithelial cells induced by high glucose |
| topic | Adiponectin receptor Telmisartan Rat renal tubular epithelial cells Peroxisome proliferator-activated receptor-γ |
| url | http://www.lcszb.com/thesisDetails?columnId=57914161&Fpath=home&index=0 |
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