Rickettsia sibirica mongolitimonae infection, Sri Lanka

Introduction. Rickettsia sibirica mongolitimonae was recently reported as a common rickettsiosis in France. Current serological evidence suggests the presence of scrub typhus and spotted fever group rickettsiosis in Sri Lanka. We detected a human case of R. sibirica mongolitimonae in Sri Lanka....

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Main Authors: Charlotte Cordier, Pierre Tattevin, Caroline Leyer, Marine Cailleaux, Didier Raoult, Emmanouil Angelakis
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2017-09-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/8743
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author Charlotte Cordier
Pierre Tattevin
Caroline Leyer
Marine Cailleaux
Didier Raoult
Emmanouil Angelakis
author_facet Charlotte Cordier
Pierre Tattevin
Caroline Leyer
Marine Cailleaux
Didier Raoult
Emmanouil Angelakis
author_sort Charlotte Cordier
collection DOAJ
description Introduction. Rickettsia sibirica mongolitimonae was recently reported as a common rickettsiosis in France. Current serological evidence suggests the presence of scrub typhus and spotted fever group rickettsiosis in Sri Lanka. We detected a human case of R. sibirica mongolitimonae in Sri Lanka. Methodology. A skin biopsy of the eschar was tested for the presence of Rickettsia spp. using qPCR assay targeting a 109-bp fragment of a hypothetical protein and by PCR amplification and sequencing targeting the ompA gene. Results. A 30-year-old woman who had just returned from travel to a jungle in Sri Lanka was evaluated as an outpatient for fever. Examination revealed an enlarged axillary lymph node, a maculopapular rash and an eschar at her left flank and a skin biopsy of the eschar was performed. The skin biopsy was positive for the presence of Rickettsia spp. by qPCR and PCR amplification and sequencing targeting the ompA gene revealed R. sibirica mongolitimonae. Immunofluorescence assay on an acute serum sample for spotted fever group rickettsial antigens (Rickettsia conorii conorii, R. sibirica mongolitimonae, Rickettsia felis) and typhus group rickettsiae (Rickettsia typhi) was negative. The patient was treated by oral doxycycline (200 mg/day) for one week. Conclusions. R. sibirica mongolitimonae should be considered in the differential diagnosis of patients with suspected rickettsiosis in, or returning from, Sri Lanka.
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spelling doaj-art-447334fc4aa04f2cb6a752957923ddc52025-08-20T02:14:17ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-09-01110810.3855/jidc.8743Rickettsia sibirica mongolitimonae infection, Sri LankaCharlotte Cordier0Pierre Tattevin1Caroline Leyer2Marine Cailleaux3Didier Raoult4Emmanouil Angelakis5URMITE CNRS-IRD 198 UMR 6236, Aix Marseille University, Marseille, FranceHospital Pontchaillou, Rennes, FranceHospital Pontchaillou, Rennes, FranceHospital Pontchaillou, Rennes, FranceURMITE CNRS-IRD 198 UMR 6236, Aix Marseille University, Marseille, FranceURMITE CNRS-IRD 198 UMR 6236, Aix Marseille University, Marseille, France Introduction. Rickettsia sibirica mongolitimonae was recently reported as a common rickettsiosis in France. Current serological evidence suggests the presence of scrub typhus and spotted fever group rickettsiosis in Sri Lanka. We detected a human case of R. sibirica mongolitimonae in Sri Lanka. Methodology. A skin biopsy of the eschar was tested for the presence of Rickettsia spp. using qPCR assay targeting a 109-bp fragment of a hypothetical protein and by PCR amplification and sequencing targeting the ompA gene. Results. A 30-year-old woman who had just returned from travel to a jungle in Sri Lanka was evaluated as an outpatient for fever. Examination revealed an enlarged axillary lymph node, a maculopapular rash and an eschar at her left flank and a skin biopsy of the eschar was performed. The skin biopsy was positive for the presence of Rickettsia spp. by qPCR and PCR amplification and sequencing targeting the ompA gene revealed R. sibirica mongolitimonae. Immunofluorescence assay on an acute serum sample for spotted fever group rickettsial antigens (Rickettsia conorii conorii, R. sibirica mongolitimonae, Rickettsia felis) and typhus group rickettsiae (Rickettsia typhi) was negative. The patient was treated by oral doxycycline (200 mg/day) for one week. Conclusions. R. sibirica mongolitimonae should be considered in the differential diagnosis of patients with suspected rickettsiosis in, or returning from, Sri Lanka. https://jidc.org/index.php/journal/article/view/8743Rickettsia sibirica mongolitimonaeskin biopsyrope-like lymphangitis-associated rickettsiosis
spellingShingle Charlotte Cordier
Pierre Tattevin
Caroline Leyer
Marine Cailleaux
Didier Raoult
Emmanouil Angelakis
Rickettsia sibirica mongolitimonae infection, Sri Lanka
Journal of Infection in Developing Countries
Rickettsia sibirica mongolitimonae
skin biopsy
rope-like lymphangitis-associated rickettsiosis
title Rickettsia sibirica mongolitimonae infection, Sri Lanka
title_full Rickettsia sibirica mongolitimonae infection, Sri Lanka
title_fullStr Rickettsia sibirica mongolitimonae infection, Sri Lanka
title_full_unstemmed Rickettsia sibirica mongolitimonae infection, Sri Lanka
title_short Rickettsia sibirica mongolitimonae infection, Sri Lanka
title_sort rickettsia sibirica mongolitimonae infection sri lanka
topic Rickettsia sibirica mongolitimonae
skin biopsy
rope-like lymphangitis-associated rickettsiosis
url https://jidc.org/index.php/journal/article/view/8743
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