Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
Sixty male Wistar rats were fed a control or an ethanol-containing diet in groups C or E. The fat compositions were adjusted with 25% or 57% fish oil substituted for olive oil in groups CF25, CF57, EF25, and EF57. Hepatic thiobarbituric acid-reactive substance (TBARS) levels, cytochrome P450 2E1 pro...
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/5801768 |
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| author | Yi-Wen Chien Hsiang-Chi Peng Ya-Ling Chen Man-Hui Pai Hsiao-Yun Wang Hsiao-Li Chuang Suh-Ching Yang |
| author_facet | Yi-Wen Chien Hsiang-Chi Peng Ya-Ling Chen Man-Hui Pai Hsiao-Yun Wang Hsiao-Li Chuang Suh-Ching Yang |
| author_sort | Yi-Wen Chien |
| collection | DOAJ |
| description | Sixty male Wistar rats were fed a control or an ethanol-containing diet in groups C or E. The fat compositions were adjusted with 25% or 57% fish oil substituted for olive oil in groups CF25, CF57, EF25, and EF57. Hepatic thiobarbituric acid-reactive substance (TBARS) levels, cytochrome P450 2E1 protein expression, and tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, and IL-10 levels, as well as intracellular adhesion molecule (ICAM)-1 levels were significantly elevated, whereas plasma adiponectin level was significantly reduced in group E (p<0.05). Hepatic histopathological scores of fatty change and inflammation, in group E were significantly higher than those of group C (p<0.05). Hepatic TBARS, plasma ICAM-1, and hepatic TNF-α, IL-1β, and IL-10 levels were significantly lower, and plasma adiponectin levels were significantly higher in groups EF25 and EF57 than those in group E (p<0.05). The immunoreactive area of the intestinal tight junction protein, ZO-1, showed no change between groups C and E. Only group CF57 displayed a significantly higher ZO-1 immunoreactive area compared to group C (p=0.0415). 25% or 57% fish oil substituted for dietary olive oil could prevent ethanol-induced liver damage in rats, but the mechanism might not be related to intestinal tight junction ZO-1 expression. |
| format | Article |
| id | doaj-art-44701c2e00fa4187ba5fe0056ddce630 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-44701c2e00fa4187ba5fe0056ddce6302025-02-03T01:21:45ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/58017685801768Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed RatsYi-Wen Chien0Hsiang-Chi Peng1Ya-Ling Chen2Man-Hui Pai3Hsiao-Yun Wang4Hsiao-Li Chuang5Suh-Ching Yang6School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, TaiwanDepartment of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Taoyuan 333, TaiwanDepartment of Anatomy, Taipei Medical University, Taipei 110, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, TaiwanNational Applied Research Laboratories, National Laboratory Animal Center, Taipei 115, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, TaiwanSixty male Wistar rats were fed a control or an ethanol-containing diet in groups C or E. The fat compositions were adjusted with 25% or 57% fish oil substituted for olive oil in groups CF25, CF57, EF25, and EF57. Hepatic thiobarbituric acid-reactive substance (TBARS) levels, cytochrome P450 2E1 protein expression, and tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, and IL-10 levels, as well as intracellular adhesion molecule (ICAM)-1 levels were significantly elevated, whereas plasma adiponectin level was significantly reduced in group E (p<0.05). Hepatic histopathological scores of fatty change and inflammation, in group E were significantly higher than those of group C (p<0.05). Hepatic TBARS, plasma ICAM-1, and hepatic TNF-α, IL-1β, and IL-10 levels were significantly lower, and plasma adiponectin levels were significantly higher in groups EF25 and EF57 than those in group E (p<0.05). The immunoreactive area of the intestinal tight junction protein, ZO-1, showed no change between groups C and E. Only group CF57 displayed a significantly higher ZO-1 immunoreactive area compared to group C (p=0.0415). 25% or 57% fish oil substituted for dietary olive oil could prevent ethanol-induced liver damage in rats, but the mechanism might not be related to intestinal tight junction ZO-1 expression.http://dx.doi.org/10.1155/2017/5801768 |
| spellingShingle | Yi-Wen Chien Hsiang-Chi Peng Ya-Ling Chen Man-Hui Pai Hsiao-Yun Wang Hsiao-Li Chuang Suh-Ching Yang Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats Mediators of Inflammation |
| title | Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats |
| title_full | Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats |
| title_fullStr | Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats |
| title_full_unstemmed | Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats |
| title_short | Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats |
| title_sort | different dietary proportions of fish oil regulate inflammatory factors but do not change intestinal tight junction zo 1 expression in ethanol fed rats |
| url | http://dx.doi.org/10.1155/2017/5801768 |
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