Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.

Background Urinary tract infections (UTIs) are among the most common bacterial infections affecting people of all ages. The increasing prevalence of antimicrobial resistance among uropathogens presents a major clinical challenge, particularly in empirical treatment. This study aimed to identify the...

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Main Authors: Dr. Nazia Begum Mohammed, Richa Choudary Cherukuri, Dr. Jyothi pendyala, Dr. K. Parameswari
Format: Article
Language:English
Published: Student's Journal of Health Research 2025-06-01
Series:Student's Journal of Health Research Africa
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Online Access:https://sjhresearchafrica.org/index.php/public-html/article/view/1859/1492
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author Dr. Nazia Begum Mohammed
Richa Choudary Cherukuri
Dr. Jyothi pendyala
Dr. K. Parameswari
author_facet Dr. Nazia Begum Mohammed
Richa Choudary Cherukuri
Dr. Jyothi pendyala
Dr. K. Parameswari
author_sort Dr. Nazia Begum Mohammed
collection DOAJ
description Background Urinary tract infections (UTIs) are among the most common bacterial infections affecting people of all ages. The increasing prevalence of antimicrobial resistance among uropathogens presents a major clinical challenge, particularly in empirical treatment. This study aimed to identify the prevalence of uropathogens and evaluate their antibiotic susceptibility patterns in a tertiary care setting. Methods A cross-sectional study was conducted at a government tertiary care hospital in Guntur district, Andhra Pradesh, India. A total of 100 urine samples from patients suspected of UTIs were collected and cultured using standard microbiological techniques. Isolated bacterial pathogens were identified, and their antibiotic susceptibility profiles were determined using the Kirby-Bauer disk diffusion method. Data were recorded and analyzed using Microsoft Excel, and results were expressed in percentages and graphically represented. Results Out of 100 urine samples analyzed, 34% showed significant bacteriuria. Among the 34 positive isolates, 91.2% (31/34) were Gram-negative bacteria. Escherichia coli was the most prevalent uropathogen, accounting for 48.4% (15/31) of Gram-negative isolates, followed by Klebsiella species at 22.6% (7/31). Antibiotic susceptibility testing revealed the highest sensitivity to Nitrofurantoin (79.4%, 27/34), followed by Carbapenems (64.7%, 22/34). Resistance was notably high against commonly used antibiotics such as Ampicillin and third-generation Cephalosporins. Conclusion The findings underscore the dominance of Gram-negative bacteria, particularly E. coli, in UTIs and the growing resistance to frequently prescribed antibiotics. Nitrofurantoin and Carbapenems demonstrated relatively high effectiveness against the isolated strains, suggesting their continued role in empirical therapy. Regular surveillance and antibiogram development are essential to guide appropriate antibiotic use and combat rising antimicrobial resistance. Recommendations Promote local antibiogram usage, restrict empirical antibiotic misuse, encourage stewardship programs, update treatment guidelines periodically, and educate healthcare professionals continuously.
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spelling doaj-art-446b3255f8364897bc40c16e9d912e0a2025-08-20T02:58:23ZengStudent's Journal of Health ResearchStudent's Journal of Health Research Africa2709-99972025-06-016619https://doi.org/10.51168/sjhrafrica.v6i6.1859Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study. Dr. Nazia Begum Mohammed 0Richa Choudary Cherukuri 1Dr. Jyothi pendyala 2Dr. K. Parameswari 3Assistant Professor, Department of Microbiology, Guntur Medical College, Guntur, Andhra Pradesh, India. Phase III MBBS Student, Guntur Medical College, Guntur, Andhra Pradesh, India. Associate Professor, Department of Microbiology, Andhra Medical College, Visakhapatnam, Andhra Pradesh, India. Professor, Department of Microbiology, Nimra Institute of Medical Sciences, Ibrahimpatnam, Andhra Pradesh, India. Background Urinary tract infections (UTIs) are among the most common bacterial infections affecting people of all ages. The increasing prevalence of antimicrobial resistance among uropathogens presents a major clinical challenge, particularly in empirical treatment. This study aimed to identify the prevalence of uropathogens and evaluate their antibiotic susceptibility patterns in a tertiary care setting. Methods A cross-sectional study was conducted at a government tertiary care hospital in Guntur district, Andhra Pradesh, India. A total of 100 urine samples from patients suspected of UTIs were collected and cultured using standard microbiological techniques. Isolated bacterial pathogens were identified, and their antibiotic susceptibility profiles were determined using the Kirby-Bauer disk diffusion method. Data were recorded and analyzed using Microsoft Excel, and results were expressed in percentages and graphically represented. Results Out of 100 urine samples analyzed, 34% showed significant bacteriuria. Among the 34 positive isolates, 91.2% (31/34) were Gram-negative bacteria. Escherichia coli was the most prevalent uropathogen, accounting for 48.4% (15/31) of Gram-negative isolates, followed by Klebsiella species at 22.6% (7/31). Antibiotic susceptibility testing revealed the highest sensitivity to Nitrofurantoin (79.4%, 27/34), followed by Carbapenems (64.7%, 22/34). Resistance was notably high against commonly used antibiotics such as Ampicillin and third-generation Cephalosporins. Conclusion The findings underscore the dominance of Gram-negative bacteria, particularly E. coli, in UTIs and the growing resistance to frequently prescribed antibiotics. Nitrofurantoin and Carbapenems demonstrated relatively high effectiveness against the isolated strains, suggesting their continued role in empirical therapy. Regular surveillance and antibiogram development are essential to guide appropriate antibiotic use and combat rising antimicrobial resistance. Recommendations Promote local antibiogram usage, restrict empirical antibiotic misuse, encourage stewardship programs, update treatment guidelines periodically, and educate healthcare professionals continuously.https://sjhresearchafrica.org/index.php/public-html/article/view/1859/1492urinary tract infectionssignificant bacteriuriaescherichia colinitrofurantoinklebsiella sps
spellingShingle Dr. Nazia Begum Mohammed
Richa Choudary Cherukuri
Dr. Jyothi pendyala
Dr. K. Parameswari
Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
Student's Journal of Health Research Africa
urinary tract infections
significant bacteriuria
escherichia coli
nitrofurantoin
klebsiella sps
title Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
title_full Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
title_fullStr Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
title_full_unstemmed Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
title_short Antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital: A cross-sectional study.
title_sort antibiogram of uropathogens from cases of urinary tract infections in a tertiary care hospital a cross sectional study
topic urinary tract infections
significant bacteriuria
escherichia coli
nitrofurantoin
klebsiella sps
url https://sjhresearchafrica.org/index.php/public-html/article/view/1859/1492
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AT drjyothipendyala antibiogramofuropathogensfromcasesofurinarytractinfectionsinatertiarycarehospitalacrosssectionalstudy
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