The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.
Plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. However, few components involved in plant cell wall integrity sensing have been described to date. Using a reverse-genetic approach, we identified the Arabidopsis thalian...
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Public Library of Science (PLoS)
2017-06-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006832&type=printable |
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| author | Dieuwertje Van der Does Freddy Boutrot Timo Engelsdorf Jack Rhodes Joseph F McKenna Samantha Vernhettes Iko Koevoets Nico Tintor Manikandan Veerabagu Eva Miedes Cécile Segonzac Milena Roux Alice S Breda Christian S Hardtke Antonio Molina Martijn Rep Christa Testerink Grégory Mouille Herman Höfte Thorsten Hamann Cyril Zipfel |
| author_facet | Dieuwertje Van der Does Freddy Boutrot Timo Engelsdorf Jack Rhodes Joseph F McKenna Samantha Vernhettes Iko Koevoets Nico Tintor Manikandan Veerabagu Eva Miedes Cécile Segonzac Milena Roux Alice S Breda Christian S Hardtke Antonio Molina Martijn Rep Christa Testerink Grégory Mouille Herman Höfte Thorsten Hamann Cyril Zipfel |
| author_sort | Dieuwertje Van der Does |
| collection | DOAJ |
| description | Plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. However, few components involved in plant cell wall integrity sensing have been described to date. Using a reverse-genetic approach, we identified the Arabidopsis thaliana leucine-rich repeat receptor kinase MIK2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. Indeed, loss-of-function mik2 alleles are strongly affected in immune marker gene expression, jasmonic acid production and lignin deposition. MIK2 has both overlapping and distinct functions with THE1, a malectin-like receptor kinase previously proposed as cell wall integrity sensor. In addition, mik2 mutant plants exhibit enhanced leftward root skewing when grown on vertical plates. Notably, natural variation in MIK2 (also named LRR-KISS) has been correlated recently to mild salt stress tolerance, which we could confirm using our insertional alleles. Strikingly, both the increased root skewing and salt stress sensitivity phenotypes observed in the mik2 mutant are dependent on THE1. Finally, we found that MIK2 is required for resistance to the fungal root pathogen Fusarium oxysporum. Together, our data identify MIK2 as a novel component in cell wall integrity sensing and suggest that MIK2 is a nexus linking cell wall integrity sensing to growth and environmental cues. |
| format | Article |
| id | doaj-art-446b270c9913474bb3989ea18accff14 |
| institution | DOAJ |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-446b270c9913474bb3989ea18accff142025-08-20T03:07:20ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-06-01136e100683210.1371/journal.pgen.1006832The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.Dieuwertje Van der DoesFreddy BoutrotTimo EngelsdorfJack RhodesJoseph F McKennaSamantha VernhettesIko KoevoetsNico TintorManikandan VeerabaguEva MiedesCécile SegonzacMilena RouxAlice S BredaChristian S HardtkeAntonio MolinaMartijn RepChrista TesterinkGrégory MouilleHerman HöfteThorsten HamannCyril ZipfelPlants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. However, few components involved in plant cell wall integrity sensing have been described to date. Using a reverse-genetic approach, we identified the Arabidopsis thaliana leucine-rich repeat receptor kinase MIK2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. Indeed, loss-of-function mik2 alleles are strongly affected in immune marker gene expression, jasmonic acid production and lignin deposition. MIK2 has both overlapping and distinct functions with THE1, a malectin-like receptor kinase previously proposed as cell wall integrity sensor. In addition, mik2 mutant plants exhibit enhanced leftward root skewing when grown on vertical plates. Notably, natural variation in MIK2 (also named LRR-KISS) has been correlated recently to mild salt stress tolerance, which we could confirm using our insertional alleles. Strikingly, both the increased root skewing and salt stress sensitivity phenotypes observed in the mik2 mutant are dependent on THE1. Finally, we found that MIK2 is required for resistance to the fungal root pathogen Fusarium oxysporum. Together, our data identify MIK2 as a novel component in cell wall integrity sensing and suggest that MIK2 is a nexus linking cell wall integrity sensing to growth and environmental cues.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006832&type=printable |
| spellingShingle | Dieuwertje Van der Does Freddy Boutrot Timo Engelsdorf Jack Rhodes Joseph F McKenna Samantha Vernhettes Iko Koevoets Nico Tintor Manikandan Veerabagu Eva Miedes Cécile Segonzac Milena Roux Alice S Breda Christian S Hardtke Antonio Molina Martijn Rep Christa Testerink Grégory Mouille Herman Höfte Thorsten Hamann Cyril Zipfel The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. PLoS Genetics |
| title | The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. |
| title_full | The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. |
| title_fullStr | The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. |
| title_full_unstemmed | The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. |
| title_short | The Arabidopsis leucine-rich repeat receptor kinase MIK2/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses. |
| title_sort | arabidopsis leucine rich repeat receptor kinase mik2 lrr kiss connects cell wall integrity sensing root growth and response to abiotic and biotic stresses |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006832&type=printable |
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