Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli

Abstract D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E. coli strain pET22b(...

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Bibliographic Details
Main Authors: Haoran Liu, Kang Xu, ShuQi Sun, Yinbiao Wan, Bojia Zhang, Yang Song, Chuanzhuang Guo, Songsen Sui, Ruiming Wang, Piwu Li, Junqing Wang, Zhenshang Xu, Ting Wang
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Subjects:
D-allulose
D-allulose 3-epimerases
Arthrobacter globiformis M30
Lactobacillus plantarum 217-8
Fermentation optimization
Online Access:https://doi.org/10.1038/s41598-024-80561-5
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