Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model
Abstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose function because they produce little energy outside cells. Therefore, this study invest...
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| Format: | Article |
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Wiley
2025-03-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202415303 |
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| author | Hui Bang Cho Hye‐Ryoung Kim Sujeong Lee Chae Won Cho Ji‐In Park Seulki Youn Gyuwon So Sumin Kang Hye Jin Kim Keun‐Hong Park |
| author_facet | Hui Bang Cho Hye‐Ryoung Kim Sujeong Lee Chae Won Cho Ji‐In Park Seulki Youn Gyuwon So Sumin Kang Hye Jin Kim Keun‐Hong Park |
| author_sort | Hui Bang Cho |
| collection | DOAJ |
| description | Abstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose function because they produce little energy outside cells. Therefore, this study investigates whether near infrared (NIR)‐mediated nicotinamide adenine dinucleotide (NADH) pre‐treatment enhances mitochondrial function and stability in mitochondria‐donor cells prior to transplantation. Clinical applications of NADH, an essential electron donor in the oxidative phosphorylation process, are restricted due to the limited cellular uptake of NADH. To address this, a photo‐mediated method optimizes direct NADH delivery into cells and increases NADH absorption. L6 cells treated with NADH and irradiated with NIR enhanced NADH uptake, significantly improving mitochondrial energy production and function. Importantly, the improved functional characteristics of the mitochondria are maintained even after isolation from cells. Primed mitochondria, i.e., those enhanced by NIR‐mediated NADH uptake (P‐MT), are encapsulated in fusogenic liposomes and delivered into muscle cells with mitochondrial dysfunction. Compared to conventional mitochondria, P‐MT mitochondria promote greater mitochondrial recovery and muscle regeneration. These findings suggest that NIR‐mediated NADH delivery is an effective strategy for improving mitochondrial function, and has the potential to lead to novel treatments for mitochondrial disorders and muscle degeneration. |
| format | Article |
| id | doaj-art-445c2e92977d46d5af7ec510274a9463 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-445c2e92977d46d5af7ec510274a94632025-08-20T03:41:59ZengWileyAdvanced Science2198-38442025-03-011212n/an/a10.1002/advs.202415303Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction ModelHui Bang Cho0Hye‐Ryoung Kim1Sujeong Lee2Chae Won Cho3Ji‐In Park4Seulki Youn5Gyuwon So6Sumin Kang7Hye Jin Kim8Keun‐Hong Park9Department of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaDepartment of Nano‐regenerative Medical Engineering College of Life Science CHA University 6F, CHA Biocomplex, Sampyeong‐Dong, Bundang‐gu Seongnam‐si 13488 Republic of KoreaAbstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose function because they produce little energy outside cells. Therefore, this study investigates whether near infrared (NIR)‐mediated nicotinamide adenine dinucleotide (NADH) pre‐treatment enhances mitochondrial function and stability in mitochondria‐donor cells prior to transplantation. Clinical applications of NADH, an essential electron donor in the oxidative phosphorylation process, are restricted due to the limited cellular uptake of NADH. To address this, a photo‐mediated method optimizes direct NADH delivery into cells and increases NADH absorption. L6 cells treated with NADH and irradiated with NIR enhanced NADH uptake, significantly improving mitochondrial energy production and function. Importantly, the improved functional characteristics of the mitochondria are maintained even after isolation from cells. Primed mitochondria, i.e., those enhanced by NIR‐mediated NADH uptake (P‐MT), are encapsulated in fusogenic liposomes and delivered into muscle cells with mitochondrial dysfunction. Compared to conventional mitochondria, P‐MT mitochondria promote greater mitochondrial recovery and muscle regeneration. These findings suggest that NIR‐mediated NADH delivery is an effective strategy for improving mitochondrial function, and has the potential to lead to novel treatments for mitochondrial disorders and muscle degeneration.https://doi.org/10.1002/advs.202415303mitotherapymitochondrial primingmuscle regenerationNADH deliverynear‐infrared (NIR) |
| spellingShingle | Hui Bang Cho Hye‐Ryoung Kim Sujeong Lee Chae Won Cho Ji‐In Park Seulki Youn Gyuwon So Sumin Kang Hye Jin Kim Keun‐Hong Park Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model Advanced Science mitotherapy mitochondrial priming muscle regeneration NADH delivery near‐infrared (NIR) |
| title | Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model |
| title_full | Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model |
| title_fullStr | Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model |
| title_full_unstemmed | Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model |
| title_short | Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model |
| title_sort | near infrared mediated intracellular nadh delivery strengthens mitochondrial function and stability in muscle dysfunction model |
| topic | mitotherapy mitochondrial priming muscle regeneration NADH delivery near‐infrared (NIR) |
| url | https://doi.org/10.1002/advs.202415303 |
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