Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China
Abstract Background The bovine mastitis caused by Klebsiella pneumoniae (K. pneumoniae) infection, lead to high death rate and severe economic loss. Currently, the regional epidemiological characteristics of K. pneumoniae in China are still unclear, especially the surveillance data on prevalence of...
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2025-07-01
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| author | Jiayun Wang Jun Li Xing Ji Lili Zhang Ran Wang Heng Wang Tao He |
| author_facet | Jiayun Wang Jun Li Xing Ji Lili Zhang Ran Wang Heng Wang Tao He |
| author_sort | Jiayun Wang |
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| description | Abstract Background The bovine mastitis caused by Klebsiella pneumoniae (K. pneumoniae) infection, lead to high death rate and severe economic loss. Currently, the regional epidemiological characteristics of K. pneumoniae in China are still unclear, especially the surveillance data on prevalence of extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae in the cow are still limited. In this study, the epidemiology of K. pneumoniae from mastitis-infected cows in large-scale dairy farms in China were investigated. Methods A total of 108 isolates of K. pneumoniae were isolated from 763 mastitis-associated milk samples collected from dairy farms in Jiangsu, Anhui, Hebei, Fujian, Guangdong, Shanxi and Zhejiang provinces in China. The whole genomes of 108 strains of K. pneumoniae were sequenced to identify their phylogenetic relationships, antimicrobial resistance and virulence genes. The antimicrobial susceptibility was determined by micro-broth dilution method according to the Clinical Laboratory and Standards Institute (CLSI) guidelines M100-S31. Results The K. pneumoniae isolates were divided into 42 different sequence types (STs) and 43 K Locus (KL) types. No dominant ST and KL were identified in this study, reflecting the great genetic divergence in the K. pneumoniae population. While ST1049 and KL5 were relatively prevalent, potentially indicating a robust transmission capability. Among the 78 virulence genes identified, eight isolates carried the aerobactin-encoding gene cluster iucABCD/iutA (iuc), which might be hypervirulent strains. The K. pneumoniae isolates showed variable resistance levels to cefotaxime, ceftiofur, cephalexin, ceftazidime, meropenem, florfenicol, sulfamethoxazole, trimethoprim/sulfamethoxazole, amikacin, gentamicin, streptomycin and tetracycline, while sensitive to ciprofloxacin, polymyxin B or tigecycline. A total of 73 kinds of antimicrobial resistance (AMR) genes were identified, and 28 strains were determined to be ESBL-producing bacteria. The pKP83 plasmid 2, which carried bla TEM and bla CTX-M-3 genes, was highly homological to three ESBL plasmids from human. The horizontal transfer potential of pKP83 plasmid 2 was further verified by conjugation to Escherichia coli J53 and K. pneumoniae K12016. Conclusions It is necessary to conduct the regular monitoring of the antibiotic resistance of clinical K. pneumoniae from bovine mastitis, in order to prevent and control the transmission of K. pneumoniae along the food chain. |
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| spelling | doaj-art-44557f0bcd124e01817c8897c3250f202025-08-20T03:45:22ZengBMCBMC Microbiology1471-21802025-07-0125111310.1186/s12866-025-04147-5Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in ChinaJiayun Wang0Jun Li1Xing Ji2Lili Zhang3Ran Wang4Heng Wang5Tao He6Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Key Laboratory for Food Quality and Satety - State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesJiangsu Key Laboratory for Food Quality and Satety - State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesJiangsu Key Laboratory for Food Quality and Satety - State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesJiangsu Key Laboratory for Food Quality and Satety - State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Key Laboratory for Food Quality and Satety - State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural SciencesAbstract Background The bovine mastitis caused by Klebsiella pneumoniae (K. pneumoniae) infection, lead to high death rate and severe economic loss. Currently, the regional epidemiological characteristics of K. pneumoniae in China are still unclear, especially the surveillance data on prevalence of extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae in the cow are still limited. In this study, the epidemiology of K. pneumoniae from mastitis-infected cows in large-scale dairy farms in China were investigated. Methods A total of 108 isolates of K. pneumoniae were isolated from 763 mastitis-associated milk samples collected from dairy farms in Jiangsu, Anhui, Hebei, Fujian, Guangdong, Shanxi and Zhejiang provinces in China. The whole genomes of 108 strains of K. pneumoniae were sequenced to identify their phylogenetic relationships, antimicrobial resistance and virulence genes. The antimicrobial susceptibility was determined by micro-broth dilution method according to the Clinical Laboratory and Standards Institute (CLSI) guidelines M100-S31. Results The K. pneumoniae isolates were divided into 42 different sequence types (STs) and 43 K Locus (KL) types. No dominant ST and KL were identified in this study, reflecting the great genetic divergence in the K. pneumoniae population. While ST1049 and KL5 were relatively prevalent, potentially indicating a robust transmission capability. Among the 78 virulence genes identified, eight isolates carried the aerobactin-encoding gene cluster iucABCD/iutA (iuc), which might be hypervirulent strains. The K. pneumoniae isolates showed variable resistance levels to cefotaxime, ceftiofur, cephalexin, ceftazidime, meropenem, florfenicol, sulfamethoxazole, trimethoprim/sulfamethoxazole, amikacin, gentamicin, streptomycin and tetracycline, while sensitive to ciprofloxacin, polymyxin B or tigecycline. A total of 73 kinds of antimicrobial resistance (AMR) genes were identified, and 28 strains were determined to be ESBL-producing bacteria. The pKP83 plasmid 2, which carried bla TEM and bla CTX-M-3 genes, was highly homological to three ESBL plasmids from human. The horizontal transfer potential of pKP83 plasmid 2 was further verified by conjugation to Escherichia coli J53 and K. pneumoniae K12016. Conclusions It is necessary to conduct the regular monitoring of the antibiotic resistance of clinical K. pneumoniae from bovine mastitis, in order to prevent and control the transmission of K. pneumoniae along the food chain.https://doi.org/10.1186/s12866-025-04147-5Klebsiella pneumoniaeBovineMastitisGenotypingAntimicrobial resistanceVirulence |
| spellingShingle | Jiayun Wang Jun Li Xing Ji Lili Zhang Ran Wang Heng Wang Tao He Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China BMC Microbiology Klebsiella pneumoniae Bovine Mastitis Genotyping Antimicrobial resistance Virulence |
| title | Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China |
| title_full | Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China |
| title_fullStr | Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China |
| title_full_unstemmed | Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China |
| title_short | Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China |
| title_sort | antimicrobial resistance and molecular epidemiology of klebsiella pneumoniae isolated from bovine mastitis in seven provinces in china |
| topic | Klebsiella pneumoniae Bovine Mastitis Genotyping Antimicrobial resistance Virulence |
| url | https://doi.org/10.1186/s12866-025-04147-5 |
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