Comparative Analysis of Chemical Composition and Radical-Scavenging Activities in Two Wheat Cultivars
<i>Triticum aestivum</i> (wheat) is one of the most significant crops worldwide. This study compares the chemical composition and radical-scavenging activities of two cultivars of <i>T. aestivum</i>, Saekeumkang wheat (SW) and Baekkang wheat (BW). Sprouted wheatgrass extracts...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/14/22/10763 |
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| Summary: | <i>Triticum aestivum</i> (wheat) is one of the most significant crops worldwide. This study compares the chemical composition and radical-scavenging activities of two cultivars of <i>T. aestivum</i>, Saekeumkang wheat (SW) and Baekkang wheat (BW). Sprouted wheatgrass extracts of SW and BW were analyzed using assessments of total polyphenol and flavonoid contents, liquid chromatography–electrospray ionization/mass spectrometry (LC-ESI/MS), and high-performance liquid chromatography with a photodiode array (HPLC-PDA). Radical-scavenging activities were evaluated using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS·<sup>+</sup>) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The results indicated that SW had a higher total polyphenol content than BW, while no significant differences were observed regarding total flavonoid content. HPLC-PDA analysis, guided by LC-ESI/MS, identified four compounds—saponarin, schaftoside, isoorientin, and isovitexin—with isoorientin (3.02 mg/g extract) and schaftoside (4.23 mg/g extract) present in higher concentrations in SW compared to BW. In the ABTS·<sup>+</sup> assay, the two samples did not show noticeable differences, with SW displaying a scavenging ability with an IC<sub>50</sub> of 3.36 mg/mL, and BW with an IC<sub>50</sub> of 3.19 mg/mL. Contrarily, the DPPH assay results showed an inverse trend, suggesting that the radical-scavenging behavior may be influenced by the synergistic and antagonistic interactions of the compounds in SW and BW extracts. |
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| ISSN: | 2076-3417 |