Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner

The experiment was conducted during May 2022 at College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Pantnagar, Uttarakhand, India. The Steinernema abbasi PN-1 is a local isolate of entomopathogenic nematode isolated from the soil collected from Uttarakhand, I...

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Main Authors: P. Vireesha, R. Pandey, M. Shashikala, N. Mehra, S. Kumar
Format: Article
Language:English
Published: Puspa Publishing House 2024-11-01
Series:International Journal of Economic Plants
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Online Access:https://ojs.pphouse.org/index.php/IJEP/article/view/5451
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author P. Vireesha
R. Pandey
M. Shashikala
N. Mehra
S. Kumar
author_facet P. Vireesha
R. Pandey
M. Shashikala
N. Mehra
S. Kumar
author_sort P. Vireesha
collection DOAJ
description The experiment was conducted during May 2022 at College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Pantnagar, Uttarakhand, India. The Steinernema abbasi PN-1 is a local isolate of entomopathogenic nematode isolated from the soil collected from Uttarakhand, India. Under the present study, virulenceof Steinernema abbasi PN-1 against different stages of Helicoverpa armigera Hubner were tested. Virulence studies of S. abbasi PN-1 against H. armigera proved that all larval stages and pupae of H. armigera were found susceptible to the IJs of S. abbasi PN-1. There was a positive correlation between insect mortality and the nematode concentration. The S. abbasi PN-1 caused 100% larval mortality at 48–60 h of post treatment in all tested doses in laboratory. Among the larval instars, 4th instar larvae of H. armigera were more susceptible with a median lethal concentration (LC50) value of 24.37 IJs larva-1 and the median lethal time (LT50) values of 25.63 hours. The 2nd instar larvae was least susceptible with an LC50 value of 78.96 IJs larva-1 and LT50 values of 41.33 hours. The pupal stage was less susceptible than the larval stage with the LC50 value of 98.3 IJs larva-1. Our results showed that S. abbasi PN-1 can be used as efficient biological control agents against H. armigera with further field studies.
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spelling doaj-art-443d931e2c8b4cf1a0edbf7ed4a357f42025-08-20T02:39:21ZengPuspa Publishing HouseInternational Journal of Economic Plants2349-47352024-11-0111Nov, 410.23910/2/2024.5725bEfficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera HubnerP. Vireesha0R. Pandey1M. Shashikala2N. Mehra3S. Kumar4Dept. of Entomology, College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Uttarakhand (263 145), IndiaDept. of Entomology, College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Uttarakhand (263 145), IndiaDept. of Entomology, College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Uttarakhand (263 145), IndiaDept. of Entomology, College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Uttarakhand (263 145), IndiaDept. of Entomology, College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Uttarakhand (263 145), India The experiment was conducted during May 2022 at College of Agriculture, G. B. Pant University of Agriculture & Technology, U. S. Nagar, Pantnagar, Uttarakhand, India. The Steinernema abbasi PN-1 is a local isolate of entomopathogenic nematode isolated from the soil collected from Uttarakhand, India. Under the present study, virulenceof Steinernema abbasi PN-1 against different stages of Helicoverpa armigera Hubner were tested. Virulence studies of S. abbasi PN-1 against H. armigera proved that all larval stages and pupae of H. armigera were found susceptible to the IJs of S. abbasi PN-1. There was a positive correlation between insect mortality and the nematode concentration. The S. abbasi PN-1 caused 100% larval mortality at 48–60 h of post treatment in all tested doses in laboratory. Among the larval instars, 4th instar larvae of H. armigera were more susceptible with a median lethal concentration (LC50) value of 24.37 IJs larva-1 and the median lethal time (LT50) values of 25.63 hours. The 2nd instar larvae was least susceptible with an LC50 value of 78.96 IJs larva-1 and LT50 values of 41.33 hours. The pupal stage was less susceptible than the larval stage with the LC50 value of 98.3 IJs larva-1. Our results showed that S. abbasi PN-1 can be used as efficient biological control agents against H. armigera with further field studies. https://ojs.pphouse.org/index.php/IJEP/article/view/5451Helicoverpa armigera, Infective Juveniles, Steinernema abbasi PN-1
spellingShingle P. Vireesha
R. Pandey
M. Shashikala
N. Mehra
S. Kumar
Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
International Journal of Economic Plants
Helicoverpa armigera, Infective Juveniles, Steinernema abbasi PN-1
title Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
title_full Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
title_fullStr Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
title_full_unstemmed Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
title_short Efficacy of Entomopathogenic Nematode, Steinernema abbasi PN-1 against Helicoverpa armigera Hubner
title_sort efficacy of entomopathogenic nematode steinernema abbasi pn 1 against helicoverpa armigera hubner
topic Helicoverpa armigera, Infective Juveniles, Steinernema abbasi PN-1
url https://ojs.pphouse.org/index.php/IJEP/article/view/5451
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