Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease
ABSTRACT Background Disturbances in calcium and phosphorus homeostasis resulting from chronic kidney disease (CKD) may lead to atherosclerotic changes in blood vessels, potentially altering bone marrow perfusion. Our study aimed to investigate vertebral bone marrow perfusion using dynamic contrast–e...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-03-01
|
| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.70039 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850205280891043840 |
|---|---|
| author | Guo‐Shu Huang Shih‐Wei Chiang Yi‐Jen Peng Skye Hsin‐Hsien Yeh Yu‐Juei Hsu Yu‐Ching Chou Heng‐Han Chang Herng‐Sheng Lee Ying‐Chun Liu Chao‐Ying Wang |
| author_facet | Guo‐Shu Huang Shih‐Wei Chiang Yi‐Jen Peng Skye Hsin‐Hsien Yeh Yu‐Juei Hsu Yu‐Ching Chou Heng‐Han Chang Herng‐Sheng Lee Ying‐Chun Liu Chao‐Ying Wang |
| author_sort | Guo‐Shu Huang |
| collection | DOAJ |
| description | ABSTRACT Background Disturbances in calcium and phosphorus homeostasis resulting from chronic kidney disease (CKD) may lead to atherosclerotic changes in blood vessels, potentially altering bone marrow perfusion. Our study aimed to investigate vertebral bone marrow perfusion using dynamic contrast–enhanced (DCE) MRI with a pharmacokinetic model. We also measured possible changes in water and fat content and bony trabeculae using T2* quantification, MR spectroscopy (MRS), and microcomputed tomography (μCT). Methods Twelve rats were randomly separated into a normal control group and a CKD (5/6 nephrectomy) group. Their lumbar spines were imaged, with monitoring of the L5 vertebral body conducted at 0, 8, 16, 30, and 43 weeks. After Week 43, all rats were sacrificed, and histologic changes were correlated with MRI and μCT results. Results The CKD group demonstrated significantly lower A and kel values (p < 0.05), significantly increased T2* values (p < 0.05), significantly decreased fat content and trabeculation (p < 0.05), sinusoidal dilatation, and decreased adipocytes in the vertebral bone marrow. Conclusion Using quantitative MRI and μCT to assess CKD‐related arthropathy of the vertebral body is feasible. Lumbar spine bone marrow perfusion deficiency in experimental CKD may be associated with decreased fat content, increased water content, and sparse trabeculation. |
| format | Article |
| id | doaj-art-4432fbe9c88241f78def2c90f022ea2b |
| institution | OA Journals |
| issn | 2572-1143 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj-art-4432fbe9c88241f78def2c90f022ea2b2025-08-20T02:11:08ZengWileyJOR Spine2572-11432025-03-0181n/an/a10.1002/jsp2.70039Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney DiseaseGuo‐Shu Huang0Shih‐Wei Chiang1Yi‐Jen Peng2Skye Hsin‐Hsien Yeh3Yu‐Juei Hsu4Yu‐Ching Chou5Heng‐Han Chang6Herng‐Sheng Lee7Ying‐Chun Liu8Chao‐Ying Wang9Department of Radiology, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanDepartment of Radiology, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanDepartment of Pathology, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanBrain Research Center, School of Medicine National Defense Medical Center Taipei TaiwanDivision of Nephrology, Department of Medicine, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanSchool of Public Health National Defense Medical Center Taipei TaiwanDepartment and Graduate Institute of Biology and Anatomy National Defense Medical Center Taipei TaiwanDepartment of Pathology, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanDepartment of Radiology, Tri‐Service General Hospital National Defense Medical Center Taipei TaiwanDepartment and Graduate Institute of Biology and Anatomy National Defense Medical Center Taipei TaiwanABSTRACT Background Disturbances in calcium and phosphorus homeostasis resulting from chronic kidney disease (CKD) may lead to atherosclerotic changes in blood vessels, potentially altering bone marrow perfusion. Our study aimed to investigate vertebral bone marrow perfusion using dynamic contrast–enhanced (DCE) MRI with a pharmacokinetic model. We also measured possible changes in water and fat content and bony trabeculae using T2* quantification, MR spectroscopy (MRS), and microcomputed tomography (μCT). Methods Twelve rats were randomly separated into a normal control group and a CKD (5/6 nephrectomy) group. Their lumbar spines were imaged, with monitoring of the L5 vertebral body conducted at 0, 8, 16, 30, and 43 weeks. After Week 43, all rats were sacrificed, and histologic changes were correlated with MRI and μCT results. Results The CKD group demonstrated significantly lower A and kel values (p < 0.05), significantly increased T2* values (p < 0.05), significantly decreased fat content and trabeculation (p < 0.05), sinusoidal dilatation, and decreased adipocytes in the vertebral bone marrow. Conclusion Using quantitative MRI and μCT to assess CKD‐related arthropathy of the vertebral body is feasible. Lumbar spine bone marrow perfusion deficiency in experimental CKD may be associated with decreased fat content, increased water content, and sparse trabeculation.https://doi.org/10.1002/jsp2.70039CKDDCE‐MRIMRSperfusionT2* quantificationvertebral bone marrow |
| spellingShingle | Guo‐Shu Huang Shih‐Wei Chiang Yi‐Jen Peng Skye Hsin‐Hsien Yeh Yu‐Juei Hsu Yu‐Ching Chou Heng‐Han Chang Herng‐Sheng Lee Ying‐Chun Liu Chao‐Ying Wang Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease JOR Spine CKD DCE‐MRI MRS perfusion T2* quantification vertebral bone marrow |
| title | Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease |
| title_full | Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease |
| title_fullStr | Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease |
| title_full_unstemmed | Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease |
| title_short | Assessment of Vertebral Bone Marrow Perfusion, Fat/Water Content, and Trabecular Bone Changes Using Multimodal MRI and Micro‐CT in a Rat Model of Chronic Kidney Disease |
| title_sort | assessment of vertebral bone marrow perfusion fat water content and trabecular bone changes using multimodal mri and micro ct in a rat model of chronic kidney disease |
| topic | CKD DCE‐MRI MRS perfusion T2* quantification vertebral bone marrow |
| url | https://doi.org/10.1002/jsp2.70039 |
| work_keys_str_mv | AT guoshuhuang assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT shihweichiang assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT yijenpeng assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT skyehsinhsienyeh assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT yujueihsu assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT yuchingchou assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT henghanchang assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT herngshenglee assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT yingchunliu assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease AT chaoyingwang assessmentofvertebralbonemarrowperfusionfatwatercontentandtrabecularbonechangesusingmultimodalmriandmicroctinaratmodelofchronickidneydisease |