Geographical distribution of Brucella melitensis inferred from rpoB gene variation
Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for its use in genotyping Brucella melitensis....
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The Journal of Infection in Developing Countries
2017-06-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7598 |
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| author | Kim-Kee Tan Yung-Chie Tan Li-Yen Chang Kok Wei Lee Siti Sarah Nor'e Wai-Yan Yee Chee-Choong Hoh Sazaly AbuBakar |
| author_facet | Kim-Kee Tan Yung-Chie Tan Li-Yen Chang Kok Wei Lee Siti Sarah Nor'e Wai-Yan Yee Chee-Choong Hoh Sazaly AbuBakar |
| author_sort | Kim-Kee Tan |
| collection | DOAJ |
| description |
Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for its use in genotyping Brucella melitensis.
Methodology: Complete rpoB gene sequences of globally distributed Brucella melitensis strains were analyzed. Single nucleotides polymorphisms (SNPs) of the rpoB gene sequences were identified and used to type Brucella melitensis strains.
Results: Six DNA polymorphisms were identified, of which two (nucleotides 3201 and 558) were novel. Analysis of the geographical distribution of the strains revealed a spatial clustering pattern with rpoB type 1 representing European and American strains, rpoB type 2 representing European, African, and Asian strains, rpoB type 3 representing Mediterranean strains, and rpoB type 4 representing African (C3201T) and European (C3201T/T558A) strains.
Conclusions: We report the discovery of two novel SNPs of rpoB gene that can serve as useful markers for epidemiology and geographical tracking of B. melitensis.
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| format | Article |
| id | doaj-art-442f6327359c4beb93cf47856bfab00a |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-442f6327359c4beb93cf47856bfab00a2025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-06-01110510.3855/jidc.7598Geographical distribution of Brucella melitensis inferred from rpoB gene variationKim-Kee Tan0Yung-Chie Tan1Li-Yen Chang2Kok Wei Lee3Siti Sarah Nor'e4Wai-Yan Yee5Chee-Choong Hoh6Sazaly AbuBakar7University of Malaya, Kuala Lumpur, MalaysiaCodon Genomics S/B, Seri Kembangan, MalaysiaUniversity of Malaya, Kuala Lumpur, MalaysiaCodon Genomics S/B, Seri Kembangan, MalaysiaUniversity of Malaya, Kuala Lumpur, MalaysiaCodon Genomics S/B, Seri Kembangan, MalaysiaCodon Genomics S/B, Seri Kembangan, MalaysiaUniversity of Malaya, Kuala Lumpur, Malaysia Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for its use in genotyping Brucella melitensis. Methodology: Complete rpoB gene sequences of globally distributed Brucella melitensis strains were analyzed. Single nucleotides polymorphisms (SNPs) of the rpoB gene sequences were identified and used to type Brucella melitensis strains. Results: Six DNA polymorphisms were identified, of which two (nucleotides 3201 and 558) were novel. Analysis of the geographical distribution of the strains revealed a spatial clustering pattern with rpoB type 1 representing European and American strains, rpoB type 2 representing European, African, and Asian strains, rpoB type 3 representing Mediterranean strains, and rpoB type 4 representing African (C3201T) and European (C3201T/T558A) strains. Conclusions: We report the discovery of two novel SNPs of rpoB gene that can serve as useful markers for epidemiology and geographical tracking of B. melitensis. https://jidc.org/index.php/journal/article/view/7598BrucellarpoBSNPs |
| spellingShingle | Kim-Kee Tan Yung-Chie Tan Li-Yen Chang Kok Wei Lee Siti Sarah Nor'e Wai-Yan Yee Chee-Choong Hoh Sazaly AbuBakar Geographical distribution of Brucella melitensis inferred from rpoB gene variation Journal of Infection in Developing Countries Brucella rpoB SNPs |
| title | Geographical distribution of Brucella melitensis inferred from rpoB gene variation |
| title_full | Geographical distribution of Brucella melitensis inferred from rpoB gene variation |
| title_fullStr | Geographical distribution of Brucella melitensis inferred from rpoB gene variation |
| title_full_unstemmed | Geographical distribution of Brucella melitensis inferred from rpoB gene variation |
| title_short | Geographical distribution of Brucella melitensis inferred from rpoB gene variation |
| title_sort | geographical distribution of brucella melitensis inferred from rpob gene variation |
| topic | Brucella rpoB SNPs |
| url | https://jidc.org/index.php/journal/article/view/7598 |
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