Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory
Introduction: Microbiology laboratories must provide accurate blood culture reports with rapid turnaround time (TAT) to effectively manage patients with sepsis. In this study three methods are compared for reporting blood culture results: a manual method that included use of a serum separator tube...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2010-09-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/978 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148780005916672 |
|---|---|
| author | Purabi Barman Sharmila Sengupta Shefali Singh |
| author_facet | Purabi Barman Sharmila Sengupta Shefali Singh |
| author_sort | Purabi Barman |
| collection | DOAJ |
| description |
Introduction: Microbiology laboratories must provide accurate blood culture reports with rapid turnaround time (TAT) to effectively manage patients with sepsis. In this study three methods are compared for reporting blood culture results: a manual method that included use of a serum separator tube (SST), the conventional manual, and an automated method for identification and susceptibility (ID/AST).
Methodology: Broth from positive blood culture bottles was added to an SST and then centrifuged. The pellet obtained was used to directly inoculate biochemical tests for identification and agar plates for AST on the first day of positivity. Biochemicals and AST plates were read the next day and final results reported on the second day at 24 hours. For conventional disk diffusion testing, the newly positive blood culture broth was also inoculated on solid media on the first day and incubated overnight. The next day AST by was performed as well as biochemical tests from pure colonies. These colonies were also used to inoculate panels for ID/AST using the automated MicroScan 40SI System. These results were recorded on the third day and results reported at 48 hours.
Results: The study included 851 samples Out of 106 (12.4%) positive blood cultures, 102 were included in the study; Comparison of the 3 methods showed good correlation. Identification was correctly reported in 95 (93.1%) isolates. The overall AST error rate was 3.8%,
Conclusions: The use of SST and direct from pellet inoculation reduced TAT for identification and AST results between 18 and 24 hours.
|
| format | Article |
| id | doaj-art-442e648b170f4da9900e9b6591e039ad |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-442e648b170f4da9900e9b6591e039ad2025-08-20T02:27:09ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-09-0141210.3855/jidc.978Study of a novel method to assist in early reporting of sepsis from the microbiology laboratoryPurabi Barman0Sharmila Sengupta1Shefali Singh2Super Religare Laboratories Ltd., Fortis Flt Lt Rajan Dhall Hospital, New Delhi, IndiaSuper Religare Laboratories Ltd., Fortis Flt Lt Rajan Dhall Hospital, New Delhi, IndiaSuper Religare Laboratories Ltd., Fortis Flt Lt Rajan Dhall Hospital, New Delhi, India Introduction: Microbiology laboratories must provide accurate blood culture reports with rapid turnaround time (TAT) to effectively manage patients with sepsis. In this study three methods are compared for reporting blood culture results: a manual method that included use of a serum separator tube (SST), the conventional manual, and an automated method for identification and susceptibility (ID/AST). Methodology: Broth from positive blood culture bottles was added to an SST and then centrifuged. The pellet obtained was used to directly inoculate biochemical tests for identification and agar plates for AST on the first day of positivity. Biochemicals and AST plates were read the next day and final results reported on the second day at 24 hours. For conventional disk diffusion testing, the newly positive blood culture broth was also inoculated on solid media on the first day and incubated overnight. The next day AST by was performed as well as biochemical tests from pure colonies. These colonies were also used to inoculate panels for ID/AST using the automated MicroScan 40SI System. These results were recorded on the third day and results reported at 48 hours. Results: The study included 851 samples Out of 106 (12.4%) positive blood cultures, 102 were included in the study; Comparison of the 3 methods showed good correlation. Identification was correctly reported in 95 (93.1%) isolates. The overall AST error rate was 3.8%, Conclusions: The use of SST and direct from pellet inoculation reduced TAT for identification and AST results between 18 and 24 hours. https://jidc.org/index.php/journal/article/view/978Turn around timeBlood cultureSSTautomated identification systemManual identification system. |
| spellingShingle | Purabi Barman Sharmila Sengupta Shefali Singh Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory Journal of Infection in Developing Countries Turn around time Blood culture SST automated identification system Manual identification system. |
| title | Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| title_full | Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| title_fullStr | Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| title_full_unstemmed | Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| title_short | Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| title_sort | study of a novel method to assist in early reporting of sepsis from the microbiology laboratory |
| topic | Turn around time Blood culture SST automated identification system Manual identification system. |
| url | https://jidc.org/index.php/journal/article/view/978 |
| work_keys_str_mv | AT purabibarman studyofanovelmethodtoassistinearlyreportingofsepsisfromthemicrobiologylaboratory AT sharmilasengupta studyofanovelmethodtoassistinearlyreportingofsepsisfromthemicrobiologylaboratory AT shefalisingh studyofanovelmethodtoassistinearlyreportingofsepsisfromthemicrobiologylaboratory |