Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing
Patients affected by the rare disease pseudoxanthoma elasticum (PXE) exhibit the calcification of elastic fibers in ocular, dermal, and vascular tissues. These symptoms are triggered by mutations in the ATP-binding cassette transporter subfamily C member 6 (ABCC6), whose substrate remains unknown. I...
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2025-04-01
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| author | Ricarda Plümers Svenja Jelinek Christopher Lindenkamp Michel R. Osterhage Cornelius Knabbe Doris Hendig |
| author_facet | Ricarda Plümers Svenja Jelinek Christopher Lindenkamp Michel R. Osterhage Cornelius Knabbe Doris Hendig |
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| description | Patients affected by the rare disease pseudoxanthoma elasticum (PXE) exhibit the calcification of elastic fibers in ocular, dermal, and vascular tissues. These symptoms are triggered by mutations in the ATP-binding cassette transporter subfamily C member 6 (ABCC6), whose substrate remains unknown. Interestingly, ABCC6 is predominantly expressed in the liver tissue, leading to the hypothesis that PXE is a metabolic disorder. We developed a genome-editing system targeting ABCC6 in human immortalized hepatocytes (HepIms) for further investigations. The HepIms were transfected with an <i>ABCC6</i>-specific clustered regulatory interspaced short palindromic repeat (CRISPR-Cas9) genome-editing plasmid, resulting in the identification of a heterozygous (<i>ht<sup>ABCC6</sup></i>HepIm) and a compound heterozygous (<i>cht<sup>ABCC6</sup></i>HepIm) clone. These clones were analyzed for key markers associated with the PXE pathobiochemistry. Hints of impaired lipid trafficking, defects in the extracellular matrix remodeling, the induction of calcification inhibitor expression, and the down regulation of senescence and inflammatory markers in <i>ABCC6</i>-deficienct HepIms were found. Our <i>ABCC6</i> knock-out model of HepIms provides a valuable tool for studying the metabolic characteristics of PXE in vitro. The initial analysis of the clones mirrors various features of the PXE pathobiochemistry and provides an outlook on future research approaches. |
| format | Article |
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| language | English |
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| spelling | doaj-art-441d5c726dfe4bb9a3064a2296e6156c2025-08-20T02:28:19ZengMDPI AGCells2073-44092025-04-0114857610.3390/cells14080576Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome EditingRicarda Plümers0Svenja Jelinek1Christopher Lindenkamp2Michel R. Osterhage3Cornelius Knabbe4Doris Hendig5Herz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyHerz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyHerz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyHerz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyHerz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyHerz- und Diabeteszentrum Nordrhein-Westfalen, Institut für Laboratoriums- und Transfusionsmedizin, Universitätsklinik der Ruhr-Universität Bochum, Medizinische Fakultät OWL (Universität Bielefeld), Georgstraße 11, 32545 Bad Oeynhausen, GermanyPatients affected by the rare disease pseudoxanthoma elasticum (PXE) exhibit the calcification of elastic fibers in ocular, dermal, and vascular tissues. These symptoms are triggered by mutations in the ATP-binding cassette transporter subfamily C member 6 (ABCC6), whose substrate remains unknown. Interestingly, ABCC6 is predominantly expressed in the liver tissue, leading to the hypothesis that PXE is a metabolic disorder. We developed a genome-editing system targeting ABCC6 in human immortalized hepatocytes (HepIms) for further investigations. The HepIms were transfected with an <i>ABCC6</i>-specific clustered regulatory interspaced short palindromic repeat (CRISPR-Cas9) genome-editing plasmid, resulting in the identification of a heterozygous (<i>ht<sup>ABCC6</sup></i>HepIm) and a compound heterozygous (<i>cht<sup>ABCC6</sup></i>HepIm) clone. These clones were analyzed for key markers associated with the PXE pathobiochemistry. Hints of impaired lipid trafficking, defects in the extracellular matrix remodeling, the induction of calcification inhibitor expression, and the down regulation of senescence and inflammatory markers in <i>ABCC6</i>-deficienct HepIms were found. Our <i>ABCC6</i> knock-out model of HepIms provides a valuable tool for studying the metabolic characteristics of PXE in vitro. The initial analysis of the clones mirrors various features of the PXE pathobiochemistry and provides an outlook on future research approaches.https://www.mdpi.com/2073-4409/14/8/576ABCC6pseudoxanthoma elasticumhepatocytesCRISPR Cas |
| spellingShingle | Ricarda Plümers Svenja Jelinek Christopher Lindenkamp Michel R. Osterhage Cornelius Knabbe Doris Hendig Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing Cells ABCC6 pseudoxanthoma elasticum hepatocytes CRISPR Cas |
| title | Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing |
| title_full | Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing |
| title_fullStr | Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing |
| title_full_unstemmed | Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing |
| title_short | Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing |
| title_sort | investigation on abcc6 deficient human hepatocytes generated by crispr cas9 genome editing |
| topic | ABCC6 pseudoxanthoma elasticum hepatocytes CRISPR Cas |
| url | https://www.mdpi.com/2073-4409/14/8/576 |
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