Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury

Abstract Background Magnetic resonance imaging (MRI) may be a non-invasive tool for managing warm hepatic ischemia-reperfusion injury (WHIRI). Purpose We aimed to evaluate the diagnostic utility of the R2* values derived from 3.0T blood oxygen level-dependent (BOLD) MRI in assessing the severity of...

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Main Authors: Shichao Xu, Qian Ji, Jing Xu
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Gastroenterology
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Online Access:https://doi.org/10.1186/s12876-025-03947-3
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author Shichao Xu
Qian Ji
Jing Xu
author_facet Shichao Xu
Qian Ji
Jing Xu
author_sort Shichao Xu
collection DOAJ
description Abstract Background Magnetic resonance imaging (MRI) may be a non-invasive tool for managing warm hepatic ischemia-reperfusion injury (WHIRI). Purpose We aimed to evaluate the diagnostic utility of the R2* values derived from 3.0T blood oxygen level-dependent (BOLD) MRI in assessing the severity of WHIRI. Methods Fifty healthy adult New Zealand white rabbits were randomly divided into 5 groups with 10 rabbits in each. The experimental groups (40 rabbits) underwent clamping of the hepatic artery and the portal vein for 10, 20, 30, or 40 min, followed by 6 h of reperfusion to induce WHIRI. The other 10 rabbits comprised the normal control group. All animals were underwent conventional 3.0 T and BOLD MRI. Animals were euthanized and the serum levels of biochemical indicators were determined. Correlations between R2* values, biochemical indicators, and WHIRI staging were assessed using Spearman’s rank correlation coefficient. The diagnostic efficacy of R2* values was evaluated using ROC curves. Results R2* values increased gradually with prolonged warm ischemia with significant differences across groups (F = 133.25, P < 0.05). A strong positive correlation was detected between R2* values and WHIRI staging (r = 0.878, P = 0.000). Biochemical indicators (ALT, AST, LDH, MDA, and MPO) increased significantly, while SOD levels decreased with prolonged warm ischemia (P < 0.05). R2* values exhibited a strong positive correlation with biochemical indicators (r > 0.495) and a negative correlation with SOD levels (r=-0.658). The diagnostic efficacy of the R2* values was highest for predicting WHIRI above stage S4 (AUC = 1.000). Conclusion R2* values on BOLD MRI provide a sensitive and accurate method for assessing WHIRI. The diagnostic efficacy of the R2* values was the best for predicting WHIRI above stage S4.
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spelling doaj-art-43f6b6d4c8f64c38b1c90017e3b29f412025-08-20T03:10:13ZengBMCBMC Gastroenterology1471-230X2025-05-012511810.1186/s12876-025-03947-3Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injuryShichao Xu0Qian Ji1Jing Xu2Department of General Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityDepartment of Radiology, Tianjin First Central HospitalDepartment of General Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai UniversityAbstract Background Magnetic resonance imaging (MRI) may be a non-invasive tool for managing warm hepatic ischemia-reperfusion injury (WHIRI). Purpose We aimed to evaluate the diagnostic utility of the R2* values derived from 3.0T blood oxygen level-dependent (BOLD) MRI in assessing the severity of WHIRI. Methods Fifty healthy adult New Zealand white rabbits were randomly divided into 5 groups with 10 rabbits in each. The experimental groups (40 rabbits) underwent clamping of the hepatic artery and the portal vein for 10, 20, 30, or 40 min, followed by 6 h of reperfusion to induce WHIRI. The other 10 rabbits comprised the normal control group. All animals were underwent conventional 3.0 T and BOLD MRI. Animals were euthanized and the serum levels of biochemical indicators were determined. Correlations between R2* values, biochemical indicators, and WHIRI staging were assessed using Spearman’s rank correlation coefficient. The diagnostic efficacy of R2* values was evaluated using ROC curves. Results R2* values increased gradually with prolonged warm ischemia with significant differences across groups (F = 133.25, P < 0.05). A strong positive correlation was detected between R2* values and WHIRI staging (r = 0.878, P = 0.000). Biochemical indicators (ALT, AST, LDH, MDA, and MPO) increased significantly, while SOD levels decreased with prolonged warm ischemia (P < 0.05). R2* values exhibited a strong positive correlation with biochemical indicators (r > 0.495) and a negative correlation with SOD levels (r=-0.658). The diagnostic efficacy of the R2* values was highest for predicting WHIRI above stage S4 (AUC = 1.000). Conclusion R2* values on BOLD MRI provide a sensitive and accurate method for assessing WHIRI. The diagnostic efficacy of the R2* values was the best for predicting WHIRI above stage S4.https://doi.org/10.1186/s12876-025-03947-3Hepatic warm ischemia-reperfusion injuryBOLD MRIR2* valueBiochemical indicatorsDiagnostic efficacy
spellingShingle Shichao Xu
Qian Ji
Jing Xu
Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
BMC Gastroenterology
Hepatic warm ischemia-reperfusion injury
BOLD MRI
R2* value
Biochemical indicators
Diagnostic efficacy
title Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
title_full Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
title_fullStr Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
title_full_unstemmed Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
title_short Evaluating the diagnostic significance of the R2* value on 3.0T MRI for assessing the severity of warm hepatic ischemia-reperfusion injury
title_sort evaluating the diagnostic significance of the r2 value on 3 0t mri for assessing the severity of warm hepatic ischemia reperfusion injury
topic Hepatic warm ischemia-reperfusion injury
BOLD MRI
R2* value
Biochemical indicators
Diagnostic efficacy
url https://doi.org/10.1186/s12876-025-03947-3
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