Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia
The methylation status of the tumor suppressor genes RASSF1A, RARβ2, and SEMA3B was studied in the samples of cancer and its histologically normal tissue of the breast and ovaries. The high rate of abnormal methylation of the CpG islet in the RASSF1A, RARβ2, and SEMA3B genes was found in the tumors...
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ABV-press
2014-07-01
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| Series: | Опухоли женской репродуктивной системы |
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| Online Access: | https://ojrs.abvpress.ru/ojrs/article/view/81 |
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| author | T. P. Kazubskaya V. I. Loginov D. S. Khodyrev V. D. Ermilova Yu. G. Payanidi N. V. Chkhikvadze V. Yu. Selchuk E. A. Braga |
| author_facet | T. P. Kazubskaya V. I. Loginov D. S. Khodyrev V. D. Ermilova Yu. G. Payanidi N. V. Chkhikvadze V. Yu. Selchuk E. A. Braga |
| author_sort | T. P. Kazubskaya |
| collection | DOAJ |
| description | The methylation status of the tumor suppressor genes RASSF1A, RARβ2, and SEMA3B was studied in the samples of cancer and its histologically normal tissue of the breast and ovaries. The high rate of abnormal methylation of the CpG islet in the RASSF1A, RARβ2, and SEMA3B genes was found in the tumors of the breast (78% (32/41), 46% (26/56), and 35% (22/65), respectively) and ovaries 73% (33/45), 30% (15/50), and 50% (25/51). Hypermethylation in the CpG islets belonging to the RASSF1A and RARβ2 genes was first ascertained in 90% of the patients with polyneoplasms involving the breast and ovaries. Abnormal methylation of the promotor region of the RASSF1A gene was shown to be detectable in preclinical-stage and anaplasia-degree breast and ovarian cancer. There was a correlation of the rate of methylation in the promoter regions of the RARβ2 and SEMA3B genes with clinical-stage and anaplasia-degree breast and ovarian cancer. Analysis of gene methylation in biological fluids provides considerable opportunity to use methylation of DNA as a marker in clinical practice. |
| format | Article |
| id | doaj-art-43eb52fcc502467487c6a012e9234ce8 |
| institution | Kabale University |
| issn | 1994-4098 1999-8627 |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли женской репродуктивной системы |
| spelling | doaj-art-43eb52fcc502467487c6a012e9234ce82025-08-20T03:38:25ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272014-07-0101616810.17650/1994-4098-2012-0-1-61-6896Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasiaT. P. Kazubskaya0V. I. Loginov1D. S. Khodyrev2V. D. Ermilova3Yu. G. Payanidi4N. V. Chkhikvadze5V. Yu. Selchuk6E. A. Braga7N.N. Blokhin Russian Cancer Research Cancer, Russian Academy of Medical SciencesState Research Center, State Research Institute of Genetics, MoscowState Research Center, State Research Institute of Genetics, MoscowN.N. Blokhin Russian Cancer Research Cancer, Russian Academy of Medical SciencesN.N. Blokhin Russian Cancer Research Cancer, Russian Academy of Medical SciencesN.N. Blokhin Russian Cancer Research Cancer, Russian Academy of Medical SciencesN.N. Blokhin Russian Cancer Research Cancer, Russian Academy of Medical SciencesState Research Center, State Research Institute of Genetics, MoscowThe methylation status of the tumor suppressor genes RASSF1A, RARβ2, and SEMA3B was studied in the samples of cancer and its histologically normal tissue of the breast and ovaries. The high rate of abnormal methylation of the CpG islet in the RASSF1A, RARβ2, and SEMA3B genes was found in the tumors of the breast (78% (32/41), 46% (26/56), and 35% (22/65), respectively) and ovaries 73% (33/45), 30% (15/50), and 50% (25/51). Hypermethylation in the CpG islets belonging to the RASSF1A and RARβ2 genes was first ascertained in 90% of the patients with polyneoplasms involving the breast and ovaries. Abnormal methylation of the promotor region of the RASSF1A gene was shown to be detectable in preclinical-stage and anaplasia-degree breast and ovarian cancer. There was a correlation of the rate of methylation in the promoter regions of the RARβ2 and SEMA3B genes with clinical-stage and anaplasia-degree breast and ovarian cancer. Analysis of gene methylation in biological fluids provides considerable opportunity to use methylation of DNA as a marker in clinical practice.https://ojrs.abvpress.ru/ojrs/article/view/81breast cancerovarian cancerpolyneoplasiamethylationrassf1ararβ2and sema3b genes |
| spellingShingle | T. P. Kazubskaya V. I. Loginov D. S. Khodyrev V. D. Ermilova Yu. G. Payanidi N. V. Chkhikvadze V. Yu. Selchuk E. A. Braga Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia Опухоли женской репродуктивной системы breast cancer ovarian cancer polyneoplasia methylation rassf1a rarβ2 and sema3b genes |
| title | Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia |
| title_full | Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia |
| title_fullStr | Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia |
| title_full_unstemmed | Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia |
| title_short | Methylation of the RASSF1A, RARβ2, and SEMA3B genes in epithelial breast and ovarian tumors, and in patients with polyneoplasia |
| title_sort | methylation of the rassf1a rarβ2 and sema3b genes in epithelial breast and ovarian tumors and in patients with polyneoplasia |
| topic | breast cancer ovarian cancer polyneoplasia methylation rassf1a rarβ2 and sema3b genes |
| url | https://ojrs.abvpress.ru/ojrs/article/view/81 |
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