Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce
Lettuce is a commonly consumed vegetable worldwide, due to its crisp edible organs and rich nutrition. Heat stress is known to seriously threaten its growth and development. However, research on lettuce response to heat stress focusing on molecular aspects is limited. Therefore, we aimed to understa...
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American Society for Horticultural Science (ASHS)
2024-11-01
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| Series: | Journal of the American Society for Horticultural Science |
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| Online Access: | https://journals.ashs.org/jashs/view/journals/jashs/149/6/article-p327.xml |
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| author | Shuping He Guotao Huo Guojun Ge Li Du Du Long Lijun Luo Ping Long Shiwei Wei |
| author_facet | Shuping He Guotao Huo Guojun Ge Li Du Du Long Lijun Luo Ping Long Shiwei Wei |
| author_sort | Shuping He |
| collection | DOAJ |
| description | Lettuce is a commonly consumed vegetable worldwide, due to its crisp edible organs and rich nutrition. Heat stress is known to seriously threaten its growth and development. However, research on lettuce response to heat stress focusing on molecular aspects is limited. Therefore, we aimed to understand the complex molecular mechanisms underlying the regulation of lettuce plant response to heat stress (37 °C), by transcriptomic analysis of a heat-resistant lettuce variety (K482) following heat stress treatment for 1.5 and 3 days. A total of 3798 and 4121 differentially expressed genes (DEGs) were identified at 1.5 and 3 days vs. the control after heat treatment, respectively; they were mostly enriched in protein processing in the systemic lupus erythematosus, alcoholism, neurotrophin signaling pathways, and the cell cycle. Most DEGs were NACs, HSPs, MYBs, bHLHs, and WRKYs. Transcriptome analysis showed that the NAC domain transcription factor LsNAC28, which is associated with heat response, is up-regulated during heat stress and LsNAC28 inhibited yeast growth under heat stress (37 °C). These findings provide a basis to comprehensively understand the complex molecular mechanism of lettuce response to heat stress. |
| format | Article |
| id | doaj-art-43dbb0e88dd94c04a2dc1135b8670822 |
| institution | OA Journals |
| issn | 2327-9788 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | American Society for Horticultural Science (ASHS) |
| record_format | Article |
| series | Journal of the American Society for Horticultural Science |
| spelling | doaj-art-43dbb0e88dd94c04a2dc1135b86708222025-08-20T02:14:57ZengAmerican Society for Horticultural Science (ASHS)Journal of the American Society for Horticultural Science2327-97882024-11-011496https://doi.org/10.21273/JASHS05396-24Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in LettuceShuping He0Guotao Huo1Guojun Ge2Li Du3Du Long4Lijun Luo5Ping Long6Shiwei Wei7Shanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaShanghai Agrobiological Gene Center, Shanghai 201106, ChinaLettuce is a commonly consumed vegetable worldwide, due to its crisp edible organs and rich nutrition. Heat stress is known to seriously threaten its growth and development. However, research on lettuce response to heat stress focusing on molecular aspects is limited. Therefore, we aimed to understand the complex molecular mechanisms underlying the regulation of lettuce plant response to heat stress (37 °C), by transcriptomic analysis of a heat-resistant lettuce variety (K482) following heat stress treatment for 1.5 and 3 days. A total of 3798 and 4121 differentially expressed genes (DEGs) were identified at 1.5 and 3 days vs. the control after heat treatment, respectively; they were mostly enriched in protein processing in the systemic lupus erythematosus, alcoholism, neurotrophin signaling pathways, and the cell cycle. Most DEGs were NACs, HSPs, MYBs, bHLHs, and WRKYs. Transcriptome analysis showed that the NAC domain transcription factor LsNAC28, which is associated with heat response, is up-regulated during heat stress and LsNAC28 inhibited yeast growth under heat stress (37 °C). These findings provide a basis to comprehensively understand the complex molecular mechanism of lettuce response to heat stress.https://journals.ashs.org/jashs/view/journals/jashs/149/6/article-p327.xmllactuca sativanac transcription factorthermal stresstranscriptomics |
| spellingShingle | Shuping He Guotao Huo Guojun Ge Li Du Du Long Lijun Luo Ping Long Shiwei Wei Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce Journal of the American Society for Horticultural Science lactuca sativa nac transcription factor thermal stress transcriptomics |
| title | Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce |
| title_full | Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce |
| title_fullStr | Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce |
| title_full_unstemmed | Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce |
| title_short | Identification and Preliminary Functional Analysis of Responsive Genes to Short-term Heat Stress in Lettuce |
| title_sort | identification and preliminary functional analysis of responsive genes to short term heat stress in lettuce |
| topic | lactuca sativa nac transcription factor thermal stress transcriptomics |
| url | https://journals.ashs.org/jashs/view/journals/jashs/149/6/article-p327.xml |
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