Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells
Astaxanthin (AST) is a carotenoid that has positive effects on various organs and tissues. It also exhibits a cardioprotective action. In this study, the influence of AST on the survival of H9c2 cardiomyocytes under hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and doxorubi...
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2024-11-01
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| author | Roman Krestinin Margarita Kobyakova Yulia Baburina Linda Sotnikova Olga Krestinina |
| author_facet | Roman Krestinin Margarita Kobyakova Yulia Baburina Linda Sotnikova Olga Krestinina |
| author_sort | Roman Krestinin |
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| description | Astaxanthin (AST) is a carotenoid that has positive effects on various organs and tissues. It also exhibits a cardioprotective action. In this study, the influence of AST on the survival of H9c2 cardiomyocytes under hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and doxorubicin (DOX)-induced cardiotoxicity was investigated. Under these conditions, the content of cytosolic Ca<sup>2+</sup> was measured, and changes in the area of the mitochondrial mass, as well as in the content of the voltage-dependent anion channel 1 (VDAC1), the autophagy marker LC3A/B, and the pro-apoptotic transcription factor homologous protein (CHOP), were determined. It was found that AST removed the cytotoxic effect of H<sub>2</sub>O<sub>2</sub> and DOX, while cell survival increased, and the mitochondrial mass did not differ from the control. At the same time, a decrease in the content of cytosolic Ca<sup>2+</sup> and the restoration of the VDAC1 level to values close to the control were observed. The restoration of the CHOP level suggests a reduction in endoplasmic reticulum (ER) stress in cells. The results allow us to consider AST as a potential agent in the prevention and/or treatment of cardiac diseases associated with oxidative stress. |
| format | Article |
| id | doaj-art-43da940cf9224fc6b2c4f236e53965b5 |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-43da940cf9224fc6b2c4f236e53965b52025-08-20T02:48:05ZengMDPI AGLife2075-17292024-11-011411140910.3390/life14111409Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial CellsRoman Krestinin0Margarita Kobyakova1Yulia Baburina2Linda Sotnikova3Olga Krestinina4Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, RussiaAstaxanthin (AST) is a carotenoid that has positive effects on various organs and tissues. It also exhibits a cardioprotective action. In this study, the influence of AST on the survival of H9c2 cardiomyocytes under hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and doxorubicin (DOX)-induced cardiotoxicity was investigated. Under these conditions, the content of cytosolic Ca<sup>2+</sup> was measured, and changes in the area of the mitochondrial mass, as well as in the content of the voltage-dependent anion channel 1 (VDAC1), the autophagy marker LC3A/B, and the pro-apoptotic transcription factor homologous protein (CHOP), were determined. It was found that AST removed the cytotoxic effect of H<sub>2</sub>O<sub>2</sub> and DOX, while cell survival increased, and the mitochondrial mass did not differ from the control. At the same time, a decrease in the content of cytosolic Ca<sup>2+</sup> and the restoration of the VDAC1 level to values close to the control were observed. The restoration of the CHOP level suggests a reduction in endoplasmic reticulum (ER) stress in cells. The results allow us to consider AST as a potential agent in the prevention and/or treatment of cardiac diseases associated with oxidative stress.https://www.mdpi.com/2075-1729/14/11/1409astaxanthinH9c2 cellscytotoxicitydoxorubicinmitochondriaautophagy |
| spellingShingle | Roman Krestinin Margarita Kobyakova Yulia Baburina Linda Sotnikova Olga Krestinina Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells Life astaxanthin H9c2 cells cytotoxicity doxorubicin mitochondria autophagy |
| title | Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells |
| title_full | Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells |
| title_fullStr | Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells |
| title_full_unstemmed | Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells |
| title_short | Astaxanthin Protects Against H<sub>2</sub>O<sub>2</sub>- and Doxorubicin-Induced Cardiotoxicity in H9c2 Rat Myocardial Cells |
| title_sort | astaxanthin protects against h sub 2 sub o sub 2 sub and doxorubicin induced cardiotoxicity in h9c2 rat myocardial cells |
| topic | astaxanthin H9c2 cells cytotoxicity doxorubicin mitochondria autophagy |
| url | https://www.mdpi.com/2075-1729/14/11/1409 |
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