A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i>
With inadequate water purification and sewage disposal systems, cholera poses a significant health threat in developing nations. <i>Vibrio cholerae</i> (<i>V. cholerae</i>) is one of the main microscopic organisms associated with cholera illness. If cholera is not treated, re...
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2023-05-01
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| author | Md. Yousuf Abu Saim Mohammad Saikat Md. Ekhlas Uddin |
| author_facet | Md. Yousuf Abu Saim Mohammad Saikat Md. Ekhlas Uddin |
| author_sort | Md. Yousuf |
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| description | With inadequate water purification and sewage disposal systems, cholera poses a significant health threat in developing nations. <i>Vibrio cholerae</i> (<i>V. cholerae</i>) is one of the main microscopic organisms associated with cholera illness. If cholera is not treated, renal failure, shock, hypokalemia, and pulmonary edema can occur, resulting in death in a matter of hours. The machinery of bacterial virulence factors is what causes this disease. Among the various <i>V. cholerae</i> strains, <i>V. cholerae</i> O1 is the most prevalent and pathogenic strain. The total genome succession of <i>V. cholerae</i> unravels the presence of different genes and uncharacterized proteins whose capabilities are not yet perceived. Therefore, it is essential to comprehend <i>V. cholerae</i> by analyzing the structure and annotating the function of uncharacterized proteins. The NCBI sequence of uncharacterized <i>V. cholerae</i> O1 EET91795.1 proteins was annotated for this study. The domain family, protein solubility, ligand binding sites, and other parameters were all determined using a variety of databases and computational tools. The protein’s ligand-binding sites were found, and its three-dimensional structure was modeled. According to the analysis, the hotdog family protein may play metabolic roles like thioester hydrolysis in the metabolism of fatty acids and the breakdown of two products such as phenylacetic acid and the pollutant 4-chlorobenzoate. The structural prediction of this protein and detection of binding sites suggested a potential target to uncover promising inhibitors against the protein to treat infection caused by the target strain. |
| format | Article |
| id | doaj-art-43da7229d6c548bfa3f1685674206c36 |
| institution | OA Journals |
| issn | 2673-4591 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Engineering Proceedings |
| spelling | doaj-art-43da7229d6c548bfa3f1685674206c362025-08-20T01:55:27ZengMDPI AGEngineering Proceedings2673-45912023-05-013716210.3390/ECP2023-14644A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i>Md. Yousuf0Abu Saim Mohammad Saikat1Md. Ekhlas Uddin2Department of Biochemistry and Molecular Biology, Tejgaon College, Dhaka 1215, BangladeshDepartment of Computational Biology and Bioinformatics, Advanced Bioscience Center for Collaborative Research (ABSCCR), Rajshahi 6250, BangladeshDepartment of Computational Biology and Bioinformatics, Advanced Bioscience Center for Collaborative Research (ABSCCR), Rajshahi 6250, BangladeshWith inadequate water purification and sewage disposal systems, cholera poses a significant health threat in developing nations. <i>Vibrio cholerae</i> (<i>V. cholerae</i>) is one of the main microscopic organisms associated with cholera illness. If cholera is not treated, renal failure, shock, hypokalemia, and pulmonary edema can occur, resulting in death in a matter of hours. The machinery of bacterial virulence factors is what causes this disease. Among the various <i>V. cholerae</i> strains, <i>V. cholerae</i> O1 is the most prevalent and pathogenic strain. The total genome succession of <i>V. cholerae</i> unravels the presence of different genes and uncharacterized proteins whose capabilities are not yet perceived. Therefore, it is essential to comprehend <i>V. cholerae</i> by analyzing the structure and annotating the function of uncharacterized proteins. The NCBI sequence of uncharacterized <i>V. cholerae</i> O1 EET91795.1 proteins was annotated for this study. The domain family, protein solubility, ligand binding sites, and other parameters were all determined using a variety of databases and computational tools. The protein’s ligand-binding sites were found, and its three-dimensional structure was modeled. According to the analysis, the hotdog family protein may play metabolic roles like thioester hydrolysis in the metabolism of fatty acids and the breakdown of two products such as phenylacetic acid and the pollutant 4-chlorobenzoate. The structural prediction of this protein and detection of binding sites suggested a potential target to uncover promising inhibitors against the protein to treat infection caused by the target strain.https://www.mdpi.com/2673-4591/37/1/62<i>Vibrio cholerae</i>uncharacterized proteinscharacterizationmicrobes |
| spellingShingle | Md. Yousuf Abu Saim Mohammad Saikat Md. Ekhlas Uddin A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> Engineering Proceedings <i>Vibrio cholerae</i> uncharacterized proteins characterization microbes |
| title | A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> |
| title_full | A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> |
| title_fullStr | A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> |
| title_full_unstemmed | A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> |
| title_short | A Bioinformatic Approach for Molecular Characterization and Functional Annotation of an Uncharacterized Protein from <i>Vibrio cholerae</i> |
| title_sort | bioinformatic approach for molecular characterization and functional annotation of an uncharacterized protein from i vibrio cholerae i |
| topic | <i>Vibrio cholerae</i> uncharacterized proteins characterization microbes |
| url | https://www.mdpi.com/2673-4591/37/1/62 |
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