Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L.
Introduction. Phytosomes containing a complex of plant components with phospholipids are highly soluble in lipid and aqueous media, overcome skin barriers well, and enhance the bioavailability of substances with low solubility. For phytoecdysteroids, substances with a wide range of pharmacological a...
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2023-09-01
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| Series: | Разработка и регистрация лекарственных средств |
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| Online Access: | https://www.pharmjournal.ru/jour/article/view/2161 |
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| author | E. I. Molokhova D. E. Lipin Yu. V. Sorokina V. G. Luzhanin |
| author_facet | E. I. Molokhova D. E. Lipin Yu. V. Sorokina V. G. Luzhanin |
| author_sort | E. I. Molokhova |
| collection | DOAJ |
| description | Introduction. Phytosomes containing a complex of plant components with phospholipids are highly soluble in lipid and aqueous media, overcome skin barriers well, and enhance the bioavailability of substances with low solubility. For phytoecdysteroids, substances with a wide range of pharmacological actions in the course of biopharmaceutical research, the prospects of creating nanoscale liposomal forms have been shown.Aim. Development of conditions for obtaining phytosomes with ecdysteroids of Serratulae coronatae L. and assessment of their quality.Materials and methods. The amount of ecdysteroids isolated from Serratulae coronatae L., the main of which is 20-hydroxyecdysone (at least 75 %). Large multilayer vesicles were obtained by forming a lipid film with further homogenization in an ultrasonic bath. The particle size, polydispersity index, zeta potential (ζ-potential) were determined on the device Zetasizer Nano ZS (Malvern Instruments Ltd., United Kingdom). The degree of inclusion of the active component was estimated by HPLC.Results and discussion. The composition has been developed and technological stages have been proposed to obtain phytosomes (small single-layer vesicles) with a diameter of 130–170 nm and an IP ≤ 0.3. The formation of hydrogen bonds in the structure of phytosomes with the main ecdysteroid of Cimicifuga racemosa, 20-hydroxyecdysone, has been confirmed using NMR spectrometry. The degree of encapsulation of Echinops ritro ecdysteroids in phytosomes was 88 and 84 % after one year of storage.Conclusion. The technology of phytosomes with ecdysteroids of the Serratulae coronatae L. has been developed. The stability of the resulting phytosomal complex indicates the possibility of creating soft dosage forms based on it. |
| format | Article |
| id | doaj-art-43d4ba384de843049924ffa8244080dc |
| institution | Kabale University |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| publishDate | 2023-09-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj-art-43d4ba384de843049924ffa8244080dc2025-08-20T03:59:04ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492023-09-010010.33380/2305-2066-2025-14-3-20721380Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L.E. I. Molokhova0D. E. Lipin1Yu. V. Sorokina2V. G. Luzhanin3Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian FederationFederal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation; LLC "Izvarino Pharma"Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian FederationFederal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian FederationIntroduction. Phytosomes containing a complex of plant components with phospholipids are highly soluble in lipid and aqueous media, overcome skin barriers well, and enhance the bioavailability of substances with low solubility. For phytoecdysteroids, substances with a wide range of pharmacological actions in the course of biopharmaceutical research, the prospects of creating nanoscale liposomal forms have been shown.Aim. Development of conditions for obtaining phytosomes with ecdysteroids of Serratulae coronatae L. and assessment of their quality.Materials and methods. The amount of ecdysteroids isolated from Serratulae coronatae L., the main of which is 20-hydroxyecdysone (at least 75 %). Large multilayer vesicles were obtained by forming a lipid film with further homogenization in an ultrasonic bath. The particle size, polydispersity index, zeta potential (ζ-potential) were determined on the device Zetasizer Nano ZS (Malvern Instruments Ltd., United Kingdom). The degree of inclusion of the active component was estimated by HPLC.Results and discussion. The composition has been developed and technological stages have been proposed to obtain phytosomes (small single-layer vesicles) with a diameter of 130–170 nm and an IP ≤ 0.3. The formation of hydrogen bonds in the structure of phytosomes with the main ecdysteroid of Cimicifuga racemosa, 20-hydroxyecdysone, has been confirmed using NMR spectrometry. The degree of encapsulation of Echinops ritro ecdysteroids in phytosomes was 88 and 84 % after one year of storage.Conclusion. The technology of phytosomes with ecdysteroids of the Serratulae coronatae L. has been developed. The stability of the resulting phytosomal complex indicates the possibility of creating soft dosage forms based on it.https://www.pharmjournal.ru/jour/article/view/2161phytosomesphytoecdysteroidsparticle sizepolydispersity indexζ-potential |
| spellingShingle | E. I. Molokhova D. E. Lipin Yu. V. Sorokina V. G. Luzhanin Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. Разработка и регистрация лекарственных средств phytosomes phytoecdysteroids particle size polydispersity index ζ-potential |
| title | Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. |
| title_full | Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. |
| title_fullStr | Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. |
| title_full_unstemmed | Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. |
| title_short | Development and evaluation of the quality of phytosomes with ecdysteroids <i>Seratula coronata</i> L. |
| title_sort | development and evaluation of the quality of phytosomes with ecdysteroids i seratula coronata i l |
| topic | phytosomes phytoecdysteroids particle size polydispersity index ζ-potential |
| url | https://www.pharmjournal.ru/jour/article/view/2161 |
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