Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study

Purpose To investigate the association between the non-HDL/HDL cholesterol ratio (NHHR) and gestational diabetes mellitus (GDM) risk, and assess NHHR’s predictive utility as a novel biomarker in U.S. pregnant women.Patients and methods A cross-sectional analysis of 1590 pregnant women from NHANES (1...

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Main Authors: Fangfang Shan, Mengmeng Ye, Haochan Wu, Hui Zhou, Zhaoxia Zhong, Yibo Wu
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:The Journal of Maternal-Fetal & Neonatal Medicine
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Online Access:https://www.tandfonline.com/doi/10.1080/14767058.2025.2516261
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author Fangfang Shan
Mengmeng Ye
Haochan Wu
Hui Zhou
Zhaoxia Zhong
Yibo Wu
author_facet Fangfang Shan
Mengmeng Ye
Haochan Wu
Hui Zhou
Zhaoxia Zhong
Yibo Wu
author_sort Fangfang Shan
collection DOAJ
description Purpose To investigate the association between the non-HDL/HDL cholesterol ratio (NHHR) and gestational diabetes mellitus (GDM) risk, and assess NHHR’s predictive utility as a novel biomarker in U.S. pregnant women.Patients and methods A cross-sectional analysis of 1590 pregnant women from NHANES (1999–2018) was conducted. GDM was defined as fasting glucose ≥5.1 mmol/L. NHHR was calculated as (total cholesterol – HDL-C)/HDL-C. Multivariable logistic regression, restricted cubic spline (RCS) models, and ROC analysis were used to evaluate associations, threshold effects, and diagnostic accuracy, incorporating NHANES sampling weights.Results The highest NHHR quartile was associated with 4.48-fold higher odds of GDM (95% CI: 2.72–7.39, p < 0.001) after full adjustment. A nonlinear dose-response relationship with a threshold at NHHR = 2.267 was identified; above this threshold, GDM odds rose sharply (OR = 2.6, 95% CI: 2.0–3.3). Subgroup analyses confirmed consistency across age, ethnicity, and BMI (p for interaction > 0.05). NHHR demonstrated limited standalone diagnostic accuracy (AUC = 0.549, 95% CI: 0.510–0.587).Conclusion While NHHR demonstrates limited standalone diagnostic accuracy (AUC = 0.549), its specificity for metabolic dysregulation suggests potential as a complementary biomarker in multi-parameter models.
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spelling doaj-art-43d1ada3793945e487dc19d1b4be04fd2025-08-20T03:22:58ZengTaylor & Francis GroupThe Journal of Maternal-Fetal & Neonatal Medicine1476-70581476-49542025-12-0138110.1080/14767058.2025.2516261Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional studyFangfang Shan0Mengmeng Ye1Haochan Wu2Hui Zhou3Zhaoxia Zhong4Yibo Wu5Obstetrics, Gynecology and Reproduction Research Center, Affiliated Hospital of Jiangnan University, Wuxi, P.R. ChinaObstetrics, Gynecology and Reproduction Research Center, Affiliated Hospital of Jiangnan University, Wuxi, P.R. ChinaObstetrics, Gynecology and Reproduction Research Center, Affiliated Hospital of Jiangnan University, Wuxi, P.R. ChinaObstetrics, Gynecology and Reproduction Research Center, Affiliated Hospital of Jiangnan University, Wuxi, P.R. ChinaDepartment of Obstetrics and Gynecology, Donghai People’s Hospital, Donghai, P.R. ChinaObstetrics, Gynecology and Reproduction Research Center, Affiliated Hospital of Jiangnan University, Wuxi, P.R. ChinaPurpose To investigate the association between the non-HDL/HDL cholesterol ratio (NHHR) and gestational diabetes mellitus (GDM) risk, and assess NHHR’s predictive utility as a novel biomarker in U.S. pregnant women.Patients and methods A cross-sectional analysis of 1590 pregnant women from NHANES (1999–2018) was conducted. GDM was defined as fasting glucose ≥5.1 mmol/L. NHHR was calculated as (total cholesterol – HDL-C)/HDL-C. Multivariable logistic regression, restricted cubic spline (RCS) models, and ROC analysis were used to evaluate associations, threshold effects, and diagnostic accuracy, incorporating NHANES sampling weights.Results The highest NHHR quartile was associated with 4.48-fold higher odds of GDM (95% CI: 2.72–7.39, p < 0.001) after full adjustment. A nonlinear dose-response relationship with a threshold at NHHR = 2.267 was identified; above this threshold, GDM odds rose sharply (OR = 2.6, 95% CI: 2.0–3.3). Subgroup analyses confirmed consistency across age, ethnicity, and BMI (p for interaction > 0.05). NHHR demonstrated limited standalone diagnostic accuracy (AUC = 0.549, 95% CI: 0.510–0.587).Conclusion While NHHR demonstrates limited standalone diagnostic accuracy (AUC = 0.549), its specificity for metabolic dysregulation suggests potential as a complementary biomarker in multi-parameter models.https://www.tandfonline.com/doi/10.1080/14767058.2025.2516261Lipid metabolisminsulin resistancepregnancy complicationsNon-HDL/HDL Cholesterol Ratio (NHHR)Gestational Diabetes Mellitus (GDM)​
spellingShingle Fangfang Shan
Mengmeng Ye
Haochan Wu
Hui Zhou
Zhaoxia Zhong
Yibo Wu
Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
The Journal of Maternal-Fetal & Neonatal Medicine
Lipid metabolism
insulin resistance
pregnancy complications
Non-HDL/HDL Cholesterol Ratio (NHHR)
Gestational Diabetes Mellitus (GDM)​
title Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
title_full Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
title_fullStr Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
title_full_unstemmed Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
title_short Non-HDL/HDL cholesterol ratio (NHHR) as a novel predictor of gestational diabetes mellitus: a NHANES-based cross-sectional study
title_sort non hdl hdl cholesterol ratio nhhr as a novel predictor of gestational diabetes mellitus a nhanes based cross sectional study
topic Lipid metabolism
insulin resistance
pregnancy complications
Non-HDL/HDL Cholesterol Ratio (NHHR)
Gestational Diabetes Mellitus (GDM)​
url https://www.tandfonline.com/doi/10.1080/14767058.2025.2516261
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