I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury.
Acute mesenteric ischemia (AMI) is a life-threatening condition that can result in multiple organ injury and death. A timely diagnosis and treatment would have a significant impact on the morbidity and mortality in high-risk patient population. The purpose of this study was to investigate if intesti...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0115242 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849726532982931456 |
|---|---|
| author | Rachel G Khadaroo Spyridon Fortis Saad Y Salim Catherine Streutker Thomas A Churchill Haibo Zhang |
| author_facet | Rachel G Khadaroo Spyridon Fortis Saad Y Salim Catherine Streutker Thomas A Churchill Haibo Zhang |
| author_sort | Rachel G Khadaroo |
| collection | DOAJ |
| description | Acute mesenteric ischemia (AMI) is a life-threatening condition that can result in multiple organ injury and death. A timely diagnosis and treatment would have a significant impact on the morbidity and mortality in high-risk patient population. The purpose of this study was to investigate if intestinal fatty acid binding protein (I-FABP) and α-defensins can be used as biomarkers for early AMI and resultant lung injury. C57BL/6 mice were subjected to intestinal ischemia by occlusion of the superior mesenteric artery. A time course of intestinal ischemia from 0.5 to 3 h was performed and followed by reperfusion for 2 h. Additional mice were treated with N-acetyl-cysteine (NAC) at 300 mg/kg given intraperitoneally prior to reperfusion. AMI resulted in severe intestinal injury characterized by neutrophil infiltrate, myeloperoxidase (MPO) levels, cytokine/chemokine levels, and tissue histopathology. Pathologic signs of ischemia were evident at 1 h, and by 3 h of ischemia, the full thickness of the intestine mucosa had areas of coagulative necrosis. It was noted that the levels of α-defensins in intestinal tissue peaked at 1 h and I-FABP in plasma peaked at 3 h after AMI. Intestinal ischemia also resulted in lung injury in a time-dependent manner. Pretreatment with NAC decreased the levels of intestinal α-defensins and plasma I-FABP, as well as lung MPO and cytokines. In summary, the concentrations of intestinal α-defensins and plasma I-FABP predicted intestinal ischemia prior to pathological evidence of ischemia and I-FABP directly correlated with resultant lung injury. The antioxidant NAC reduced intestinal and lung injury induced by AMI, suggesting a role for oxidants in the mechanism for distant organ injury. I-FABP and α-defensins are promising biomarkers, and may guide the treatment with antioxidant in early intestinal and distal organ injury. |
| format | Article |
| id | doaj-art-43cd5162a13c4e089118e0e6bd16c5b5 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-43cd5162a13c4e089118e0e6bd16c5b52025-08-20T03:10:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11524210.1371/journal.pone.0115242I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury.Rachel G KhadarooSpyridon FortisSaad Y SalimCatherine StreutkerThomas A ChurchillHaibo ZhangAcute mesenteric ischemia (AMI) is a life-threatening condition that can result in multiple organ injury and death. A timely diagnosis and treatment would have a significant impact on the morbidity and mortality in high-risk patient population. The purpose of this study was to investigate if intestinal fatty acid binding protein (I-FABP) and α-defensins can be used as biomarkers for early AMI and resultant lung injury. C57BL/6 mice were subjected to intestinal ischemia by occlusion of the superior mesenteric artery. A time course of intestinal ischemia from 0.5 to 3 h was performed and followed by reperfusion for 2 h. Additional mice were treated with N-acetyl-cysteine (NAC) at 300 mg/kg given intraperitoneally prior to reperfusion. AMI resulted in severe intestinal injury characterized by neutrophil infiltrate, myeloperoxidase (MPO) levels, cytokine/chemokine levels, and tissue histopathology. Pathologic signs of ischemia were evident at 1 h, and by 3 h of ischemia, the full thickness of the intestine mucosa had areas of coagulative necrosis. It was noted that the levels of α-defensins in intestinal tissue peaked at 1 h and I-FABP in plasma peaked at 3 h after AMI. Intestinal ischemia also resulted in lung injury in a time-dependent manner. Pretreatment with NAC decreased the levels of intestinal α-defensins and plasma I-FABP, as well as lung MPO and cytokines. In summary, the concentrations of intestinal α-defensins and plasma I-FABP predicted intestinal ischemia prior to pathological evidence of ischemia and I-FABP directly correlated with resultant lung injury. The antioxidant NAC reduced intestinal and lung injury induced by AMI, suggesting a role for oxidants in the mechanism for distant organ injury. I-FABP and α-defensins are promising biomarkers, and may guide the treatment with antioxidant in early intestinal and distal organ injury.https://doi.org/10.1371/journal.pone.0115242 |
| spellingShingle | Rachel G Khadaroo Spyridon Fortis Saad Y Salim Catherine Streutker Thomas A Churchill Haibo Zhang I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. PLoS ONE |
| title | I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. |
| title_full | I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. |
| title_fullStr | I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. |
| title_full_unstemmed | I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. |
| title_short | I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. |
| title_sort | i fabp as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury |
| url | https://doi.org/10.1371/journal.pone.0115242 |
| work_keys_str_mv | AT rachelgkhadaroo ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury AT spyridonfortis ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury AT saadysalim ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury AT catherinestreutker ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury AT thomasachurchill ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury AT haibozhang ifabpasbiomarkerfortheearlydiagnosisofacutemesentericischemiaandresultantlunginjury |