Comparative evaluation of DNA extraction protocols for neonatal gut microbiota profiling in a resource-limited setting

Comparative evaluation of DNA extraction protocols for neonatal gut microbiota profiling in a resource-limited setting

Accurate and reproducible gut microbiome profiling depends heavily on the DNA extraction method, particularly in low-biomass samples such as neonatal stool. In this study, we evaluated the performance of three commercially available DNA extraction kits; QIAamp Fast DNA Stool Mini, DNeasy PowerSoil P...

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Bibliographic Details
Main Authors: Chinenye Akpulu, Aditya Kumar Lankapalli, Rida Toufiq, Kate Cook, Edward AR Portal, Rashida Yakubu Khalid, Aisha Mukkadas, Safiya Gambo, Aliyu Aminu, Kenneth Iregbu, Kathryn Thomson, Timothy Walsh, Kirsty Sands
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:The Microbe
Subjects:
Neonatal gut microbiota
DNA extraction
Metagenomics
Low-resource settings
Long-read sequencing
Sample preservation
Online Access:http://www.sciencedirect.com/science/article/pii/S2950194625001669
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