ESSENTIAL OILS PHARMACOPEIALIDENTIFICATION TESTS AND USES
Essential oils are recognized for their medicinal importance since ancient time and they continue to be of paramount significance, proved by the number of studies conducted in biological sciences in recent years. This review deals with the chromatographic techniques for the identification of essent...
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| Format: | Article |
| Language: | English |
| Published: |
Baqai University Press
2024-10-01
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| Series: | Baqai Journal of Health Sciences |
| Online Access: | https://journals.baqai.edu.pk/index.php/CS/article/view/99 |
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| Summary: | Essential oils are recognized for their medicinal importance since ancient time and they continue to be of
paramount significance, proved by the number of studies conducted in biological sciences in recent years.
This review deals with the chromatographic techniques for the identification of essential oils, their
components, and medicinal uses. The various identification tests for essential oils described in the British
Pharmacopoeia (BP) involve physical, chemical, and chromatographic tests. The focus of this study is
chromatographic techniques which are based on thin layer chromatography (TLC) and gas-liquid
chromatography (GLC). The techniques are presented in a tabular form which mentions its chromatographic
conditions like stationary phase, mobile phase, methods of detection and the compounds detected in different
essential oils by these techniques. Silica gel is the most commonly used stationary phase in TLC whereas
macrogol 20000, bonded macrogol 20000 or poly (dimethyl) (diethyl) siloxane supported on fused silica
as a stationary phase is commonly used in GLC. The mobile phase in TLC varies from toluene to its
combination with ethyl acetate or menthol in different ratios while the mobile phase in GLC is helium.
The detection methods used for TLC are UV light and chemical reactions whereas the detector used in
GLC is flame ionization. The components of essential oils identified by these techniques are up to 10 or
more. Almost all essential oils possess antioxidant, anticancer, antimicrobial, anti-inflammatory and insect
repellent properties along with other specific properties.
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| ISSN: | 2312-4423 2312-6884 |