3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly
Abstract A 3D‐printed origami‐inspired magnetic scaffold has been developed to investigate the influence of physical cues on guided cellular proliferation in a 3D microenvironment. Microscale channels are first constructed and populated with NIH/3T3 fibroblast and/or A549 cancer cell clusters that a...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-06-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400903 |
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| _version_ | 1849723178715185152 |
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| author | Brandon Daul Ryan Martin Phillip Glass Reza Moonesi Rad Richard Inho Joh Fanben Meng Daeha Joung |
| author_facet | Brandon Daul Ryan Martin Phillip Glass Reza Moonesi Rad Richard Inho Joh Fanben Meng Daeha Joung |
| author_sort | Brandon Daul |
| collection | DOAJ |
| description | Abstract A 3D‐printed origami‐inspired magnetic scaffold has been developed to investigate the influence of physical cues on guided cellular proliferation in a 3D microenvironment. Microscale channels are first constructed and populated with NIH/3T3 fibroblast and/or A549 cancer cell clusters that are initially bioprinted within the channels. Once these channels are fully populated, a permanent magnet is applied to fold the scaffolds. By varying the channel width and incorporating an intermediate extracellular matrix hydrogel (IE) layer along with origami folding, the scaffold provides geometric and gravitational cues to influence cellular proliferation. In both monoculture and coculture, i) cells tend to proliferate more in a tapered manner, ii) scaffolds with enhanced media flow lead to a higher volume of cell growth, and iii) cells form homogeneous distributions under gravity after dispersion. In coculture, the expansion of fibroblast clusters within their seeded channels increased, facilitating the proliferation of cancer cell clusters into the non‐seeded channels. This origami scaffold offers valuable insights into tissue engineering and cancer research, serving as a versatile tool for examining cellular interactions and growth dynamics. |
| format | Article |
| id | doaj-art-43bcaadf57de41638a73da7206c42c9f |
| institution | DOAJ |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-43bcaadf57de41638a73da7206c42c9f2025-08-20T03:11:06ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-06-011211n/an/a10.1002/admi.2024009033D Printed Magnetic Origami Scaffolds for Guided Tissue AssemblyBrandon Daul0Ryan Martin1Phillip Glass2Reza Moonesi Rad3Richard Inho Joh4Fanben Meng5Daeha Joung6Department of Physics Virginia Commonwealth University Richmond VA 23284 USADepartment of Physics Virginia Commonwealth University Richmond VA 23284 USADepartment of Physics Virginia Commonwealth University Richmond VA 23284 USADepartment of Physics Virginia Commonwealth University Richmond VA 23284 USADepartment of Physics Virginia Commonwealth University Richmond VA 23284 USADepartment of Mechanical and Materials Engineering University of Nebraska‐Lincoln Lincoln NE 68588 USADepartment of Physics Virginia Commonwealth University Richmond VA 23284 USAAbstract A 3D‐printed origami‐inspired magnetic scaffold has been developed to investigate the influence of physical cues on guided cellular proliferation in a 3D microenvironment. Microscale channels are first constructed and populated with NIH/3T3 fibroblast and/or A549 cancer cell clusters that are initially bioprinted within the channels. Once these channels are fully populated, a permanent magnet is applied to fold the scaffolds. By varying the channel width and incorporating an intermediate extracellular matrix hydrogel (IE) layer along with origami folding, the scaffold provides geometric and gravitational cues to influence cellular proliferation. In both monoculture and coculture, i) cells tend to proliferate more in a tapered manner, ii) scaffolds with enhanced media flow lead to a higher volume of cell growth, and iii) cells form homogeneous distributions under gravity after dispersion. In coculture, the expansion of fibroblast clusters within their seeded channels increased, facilitating the proliferation of cancer cell clusters into the non‐seeded channels. This origami scaffold offers valuable insights into tissue engineering and cancer research, serving as a versatile tool for examining cellular interactions and growth dynamics.https://doi.org/10.1002/admi.2024009033D coculture3D printingorigami‐inspired self‐foldingphysical cuetissue engineering |
| spellingShingle | Brandon Daul Ryan Martin Phillip Glass Reza Moonesi Rad Richard Inho Joh Fanben Meng Daeha Joung 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly Advanced Materials Interfaces 3D coculture 3D printing origami‐inspired self‐folding physical cue tissue engineering |
| title | 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly |
| title_full | 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly |
| title_fullStr | 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly |
| title_full_unstemmed | 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly |
| title_short | 3D Printed Magnetic Origami Scaffolds for Guided Tissue Assembly |
| title_sort | 3d printed magnetic origami scaffolds for guided tissue assembly |
| topic | 3D coculture 3D printing origami‐inspired self‐folding physical cue tissue engineering |
| url | https://doi.org/10.1002/admi.202400903 |
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