Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands
<i>K. pneumoniae</i> is a major cause of bovine mastitis worldwide, making it difficult to control due to its resistance to multiple drugs. <i>L. paraplantarum</i> has been explored as a promising new approach to fighting bovine mastitis. In this study, the probiotic potentia...
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2025-04-01
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| author | Jia Cheng Jingdi Tong Can Li Ziyan Wang Hao Li Meiyi Ren Jinshang Song Deyuan Song Qinna Xie Mingchao Liu |
| author_facet | Jia Cheng Jingdi Tong Can Li Ziyan Wang Hao Li Meiyi Ren Jinshang Song Deyuan Song Qinna Xie Mingchao Liu |
| author_sort | Jia Cheng |
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| description | <i>K. pneumoniae</i> is a major cause of bovine mastitis worldwide, making it difficult to control due to its resistance to multiple drugs. <i>L. paraplantarum</i> has been explored as a promising new approach to fighting bovine mastitis. In this study, the probiotic potential and safety of <i>L. paraplantarum</i> SDN1.2, as well as its ex vivo and in vivo anti-inflammatory effects against <i>K. pneumoniae</i>-induced mastitis, were comprehensively investigated using bioinformatics analyses and experimental validation methods. The results revealed that <i>L. paraplantarum</i> SDN1.2 exhibits non-hemolytic activity, is not cytotoxic, lacks virulence genes (e.g., adhesion factors, toxins, and invasion factors) and antibiotic resistance genes (e.g., beta-lactamases and tetracycline resistance genes), as supported by whole-genome sequencing, and significantly inhibits the growth of <i>K. pneumoniae</i>, as evaluated by antimicrobial tests. Following further validation in vitro, <i>L. paraplantarum</i> SDN1.2 demonstrated the capability to inhibit the adhesion and invasion of <i>K. pneumoniae</i> to bMECs. In a mouse model of <i>K. pneumoniae</i>-induced mastitis, <i>L. paraplantarum</i> SDN1.2 reduced the extent of neutrophil infiltration and inflammatory lesions. Furthermore, <i>L. paraplantarum</i> SDN1.2 pretreatment significantly reduced myeloperoxidase (MPO) activity and the expression of inflammatory cytokines (<i>IL-6</i>, <i>IL-1β</i>, and <i>TNF-a</i>) in mouse mammary gland tissue. In <i>K. pneumoniae</i>-infected bMECs, <i>L. paraplantarum</i> SDN1.2 significantly lowered lactate dehydrogenase (LDH) levels and expression of inflammatory cytokines such as <i>IL-6</i>, <i>IL-1β</i>, and <i>TNF-α</i>. The results demonstrated that the newly isolated <i>L. paraplantarum</i> SDN1.2 from bovine sources exhibits promising characteristics as a safe probiotic for the alleviation of bovine mastitis due to its safety profile and anti-inflammatory and antibacterial properties. |
| format | Article |
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| publishDate | 2025-04-01 |
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| spelling | doaj-art-43ba045a40d44c9f83a548cbd4a66aa42025-08-20T02:25:08ZengMDPI AGVeterinary Sciences2306-73812025-04-0112432310.3390/vetsci12040323Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary GlandsJia Cheng0Jingdi Tong1Can Li2Ziyan Wang3Hao Li4Meiyi Ren5Jinshang Song6Deyuan Song7Qinna Xie8Mingchao Liu9Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China<i>K. pneumoniae</i> is a major cause of bovine mastitis worldwide, making it difficult to control due to its resistance to multiple drugs. <i>L. paraplantarum</i> has been explored as a promising new approach to fighting bovine mastitis. In this study, the probiotic potential and safety of <i>L. paraplantarum</i> SDN1.2, as well as its ex vivo and in vivo anti-inflammatory effects against <i>K. pneumoniae</i>-induced mastitis, were comprehensively investigated using bioinformatics analyses and experimental validation methods. The results revealed that <i>L. paraplantarum</i> SDN1.2 exhibits non-hemolytic activity, is not cytotoxic, lacks virulence genes (e.g., adhesion factors, toxins, and invasion factors) and antibiotic resistance genes (e.g., beta-lactamases and tetracycline resistance genes), as supported by whole-genome sequencing, and significantly inhibits the growth of <i>K. pneumoniae</i>, as evaluated by antimicrobial tests. Following further validation in vitro, <i>L. paraplantarum</i> SDN1.2 demonstrated the capability to inhibit the adhesion and invasion of <i>K. pneumoniae</i> to bMECs. In a mouse model of <i>K. pneumoniae</i>-induced mastitis, <i>L. paraplantarum</i> SDN1.2 reduced the extent of neutrophil infiltration and inflammatory lesions. Furthermore, <i>L. paraplantarum</i> SDN1.2 pretreatment significantly reduced myeloperoxidase (MPO) activity and the expression of inflammatory cytokines (<i>IL-6</i>, <i>IL-1β</i>, and <i>TNF-a</i>) in mouse mammary gland tissue. In <i>K. pneumoniae</i>-infected bMECs, <i>L. paraplantarum</i> SDN1.2 significantly lowered lactate dehydrogenase (LDH) levels and expression of inflammatory cytokines such as <i>IL-6</i>, <i>IL-1β</i>, and <i>TNF-α</i>. The results demonstrated that the newly isolated <i>L. paraplantarum</i> SDN1.2 from bovine sources exhibits promising characteristics as a safe probiotic for the alleviation of bovine mastitis due to its safety profile and anti-inflammatory and antibacterial properties.https://www.mdpi.com/2306-7381/12/4/323bovine mastitis<i>Klebsiella pneumoniae</i><i>Lactiplantibacillus paraplantarum</i>anti-inflammatory |
| spellingShingle | Jia Cheng Jingdi Tong Can Li Ziyan Wang Hao Li Meiyi Ren Jinshang Song Deyuan Song Qinna Xie Mingchao Liu Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands Veterinary Sciences bovine mastitis <i>Klebsiella pneumoniae</i> <i>Lactiplantibacillus paraplantarum</i> anti-inflammatory |
| title | Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands |
| title_full | Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands |
| title_fullStr | Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands |
| title_full_unstemmed | Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands |
| title_short | Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands |
| title_sort | probiotic characterization of i lactiplantibacillus paraplantarum i sdn1 2 and its anti inflammatory effect on i klebsiella pneumoniae i infected mammary glands |
| topic | bovine mastitis <i>Klebsiella pneumoniae</i> <i>Lactiplantibacillus paraplantarum</i> anti-inflammatory |
| url | https://www.mdpi.com/2306-7381/12/4/323 |
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