Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform
Food and agricultural commodities endure consistent contamination by mycotoxins, low molecular weight fungal metabolites, which pose severe health implications to humans together with staggering economic losses. Herein, a ratiometric aptasensor was constructed using silver-coated porous silicon (Ag-...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524010393 |
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| author | Narsingh R. Nirala Sudharsan Sadhasivam Rohit Kumar Singh Edward Sionov Giorgi Shtenberg |
| author_facet | Narsingh R. Nirala Sudharsan Sadhasivam Rohit Kumar Singh Edward Sionov Giorgi Shtenberg |
| author_sort | Narsingh R. Nirala |
| collection | DOAJ |
| description | Food and agricultural commodities endure consistent contamination by mycotoxins, low molecular weight fungal metabolites, which pose severe health implications to humans together with staggering economic losses. Herein, a ratiometric aptasensor was constructed using silver-coated porous silicon (Ag-pSi) used as an efficient surface-enhanced Raman scattering (SERS) substrate. The bioassay included direct detection of fumonisin B1 (FB1), an abundant and widespread contaminant, by a specific aptamer sequence immobilized on the porous transducer. The inherent surface void and pore morphology were physically optimized to achieve a sufficient SERS effect (enhancement factor > 5 × 107). Under optimal conditions, the aptasensor exhibits high sensitivity, wide dynamic range, signal stability, selectivity and regeneration for consecutive FB1 detection (0.05 ppb, 0.1–1000 ppb, RSD of 5.2 %, no interference with competing mycotoxins and eight regeneration cycles, respectively). The efficacy of the designed aptasensor was elucidated in various spiked matrices (maize, onion, wheat and milk) with averaged recovery values of 93.3–113.6 % and satisfactory consistency with HPLC data for representative foodstuffs. Overall, the resulting validation emphasizes the transducer's reliability and suitability for practical use, including on-site analysis. |
| format | Article |
| id | doaj-art-43a30876febd48bd810a5a19c1fd8891 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-43a30876febd48bd810a5a19c1fd88912025-02-12T05:32:14ZengElsevierFood Chemistry: X2590-15752025-01-0125102151Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platformNarsingh R. Nirala0Sudharsan Sadhasivam1Rohit Kumar Singh2Edward Sionov3Giorgi Shtenberg4Institute of Agricultural Engineering, ARO, Volcani Institute, Rishon LeZion, IsraelInstitute of Postharvest and Food Sciences, ARO, Volcani Institute, Rishon LeZion, Israel; The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelInstitute of Agricultural Engineering, ARO, Volcani Institute, Rishon LeZion, IsraelInstitute of Postharvest and Food Sciences, ARO, Volcani Institute, Rishon LeZion, Israel; Corresponding authors.Institute of Agricultural Engineering, ARO, Volcani Institute, Rishon LeZion, Israel; Corresponding authors.Food and agricultural commodities endure consistent contamination by mycotoxins, low molecular weight fungal metabolites, which pose severe health implications to humans together with staggering economic losses. Herein, a ratiometric aptasensor was constructed using silver-coated porous silicon (Ag-pSi) used as an efficient surface-enhanced Raman scattering (SERS) substrate. The bioassay included direct detection of fumonisin B1 (FB1), an abundant and widespread contaminant, by a specific aptamer sequence immobilized on the porous transducer. The inherent surface void and pore morphology were physically optimized to achieve a sufficient SERS effect (enhancement factor > 5 × 107). Under optimal conditions, the aptasensor exhibits high sensitivity, wide dynamic range, signal stability, selectivity and regeneration for consecutive FB1 detection (0.05 ppb, 0.1–1000 ppb, RSD of 5.2 %, no interference with competing mycotoxins and eight regeneration cycles, respectively). The efficacy of the designed aptasensor was elucidated in various spiked matrices (maize, onion, wheat and milk) with averaged recovery values of 93.3–113.6 % and satisfactory consistency with HPLC data for representative foodstuffs. Overall, the resulting validation emphasizes the transducer's reliability and suitability for practical use, including on-site analysis.http://www.sciencedirect.com/science/article/pii/S2590157524010393AptamerFood safetyFumonisin B1Porous siliconSERS |
| spellingShingle | Narsingh R. Nirala Sudharsan Sadhasivam Rohit Kumar Singh Edward Sionov Giorgi Shtenberg Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform Food Chemistry: X Aptamer Food safety Fumonisin B1 Porous silicon SERS |
| title | Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform |
| title_full | Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform |
| title_fullStr | Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform |
| title_full_unstemmed | Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform |
| title_short | Sensitive ratiometric detection of Fumonisin B1 using a reusable Ag-pSi SERS platform |
| title_sort | sensitive ratiometric detection of fumonisin b1 using a reusable ag psi sers platform |
| topic | Aptamer Food safety Fumonisin B1 Porous silicon SERS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524010393 |
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