Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus
Abstract Pleurotus is an edible mushroom that is consumed and cultivated worldwide. Pleurotus djamor var. fuscopruinosus is newly reported in Thailand, confirmed through morphological and phylogenetic analyses. Cultivation trials showed optimal mycelial growth on PDA, with sorghum supporting the hig...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-02900-4 |
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| author | Monthien Phonemany Phongeun Sysouphanthong Narawadee Rujanapun Satyajit D. Sarker Lutfun Nahar Panupong Puttarak Poonsit Hiransai Naritsada Thongklang Rawiwan Charoensup |
| author_facet | Monthien Phonemany Phongeun Sysouphanthong Narawadee Rujanapun Satyajit D. Sarker Lutfun Nahar Panupong Puttarak Poonsit Hiransai Naritsada Thongklang Rawiwan Charoensup |
| author_sort | Monthien Phonemany |
| collection | DOAJ |
| description | Abstract Pleurotus is an edible mushroom that is consumed and cultivated worldwide. Pleurotus djamor var. fuscopruinosus is newly reported in Thailand, confirmed through morphological and phylogenetic analyses. Cultivation trials showed optimal mycelial growth on PDA, with sorghum supporting the highest spawn production. Sawdust was a suitable substrate, with fruiting bodies forming at 25–28 °C and 70–80% humidity, maturing within 4–5 days. Nutritional analysis (per 100 g dry weight) revealed 25.00 ± 1.5% protein, 15.99 ± 0.2% carbohydrates, 19.10 ± 0.9% fiber, 7.59 ± 1.0% ash, 2.07 ± 0.5% fat, and 30.25 ± 0.5% moisture. The 50% ethyl acetate extract exhibited the strongest antioxidant activity (IC50: 694.47 ± 3.92 µg/mL for DPPH; 652.92 ± 2.53 µg/mL for ABTS). The extract showed selective cytotoxicity against lung (A549, IC50 = 245.73 ± 7.60 µg/mL) and colorectal (SW480, IC50 = 382.03 ± 4.55 µg/mL) cancer cells but was less potent than doxorubicin (IC50 = 14.96 ± 1.58 µg/mL). Moderate cytotoxicity was observed in RAW 264.7 macrophages (IC50 = 213.08 ± 4.08 µg/mL). Antidiabetic potential was demonstrated through α-glucosidase inhibition, with the hot water extract (IC50 = 582.91 ± 3.0 µg/mL) showing the highest activity, followed by ethyl acetate (IC50 = 473.87 ± 1.4 µg/mL) and methanol (IC50 = 357.63 ± 3.3 µg/mL), comparable to acarbose (IC50 = 635.70 ± 4.9 µg/mL). Glucose consumption in 3T3-L1 adipocytes was enhanced (IC50 = 582.91 ± 3.0 µg/mL) but lower than metformin (IC50 = 99.58 ± 0.59 µg/mL). These results highlight P. djamor var. fuscopruinosus as a promising functional food with strong antioxidant, anticancer effects, and antidiabetic properties. Further studies should optimize cultivation, isolate bioactive compounds, assess safety, and validate therapeutic properties through in vivo studies. |
| format | Article |
| id | doaj-art-43a2ce3818fb452bbbe23bf8b2bcd88b |
| institution | OA Journals |
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| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-43a2ce3818fb452bbbe23bf8b2bcd88b2025-08-20T02:03:35ZengNature PortfolioScientific Reports2045-23222025-05-0115112010.1038/s41598-025-02900-4Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosusMonthien Phonemany0Phongeun Sysouphanthong1Narawadee Rujanapun2Satyajit D. Sarker3Lutfun Nahar4Panupong Puttarak5Poonsit Hiransai6Naritsada Thongklang7Rawiwan Charoensup8School of Science, Mae Fah Luang UniversitySchool of Science, Mae Fah Luang UniversityMedicinal Plant Innovation Center of Mae Fah, Luang UniversityCentre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores UniversityLaboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of SciencesPhytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla UniversityHerbology Research Center, Walailak UniversitySchool of Science, Mae Fah Luang UniversityMedicinal Plant Innovation Center of Mae Fah, Luang UniversityAbstract Pleurotus is an edible mushroom that is consumed and cultivated worldwide. Pleurotus djamor var. fuscopruinosus is newly reported in Thailand, confirmed through morphological and phylogenetic analyses. Cultivation trials showed optimal mycelial growth on PDA, with sorghum supporting the highest spawn production. Sawdust was a suitable substrate, with fruiting bodies forming at 25–28 °C and 70–80% humidity, maturing within 4–5 days. Nutritional analysis (per 100 g dry weight) revealed 25.00 ± 1.5% protein, 15.99 ± 0.2% carbohydrates, 19.10 ± 0.9% fiber, 7.59 ± 1.0% ash, 2.07 ± 0.5% fat, and 30.25 ± 0.5% moisture. The 50% ethyl acetate extract exhibited the strongest antioxidant activity (IC50: 694.47 ± 3.92 µg/mL for DPPH; 652.92 ± 2.53 µg/mL for ABTS). The extract showed selective cytotoxicity against lung (A549, IC50 = 245.73 ± 7.60 µg/mL) and colorectal (SW480, IC50 = 382.03 ± 4.55 µg/mL) cancer cells but was less potent than doxorubicin (IC50 = 14.96 ± 1.58 µg/mL). Moderate cytotoxicity was observed in RAW 264.7 macrophages (IC50 = 213.08 ± 4.08 µg/mL). Antidiabetic potential was demonstrated through α-glucosidase inhibition, with the hot water extract (IC50 = 582.91 ± 3.0 µg/mL) showing the highest activity, followed by ethyl acetate (IC50 = 473.87 ± 1.4 µg/mL) and methanol (IC50 = 357.63 ± 3.3 µg/mL), comparable to acarbose (IC50 = 635.70 ± 4.9 µg/mL). Glucose consumption in 3T3-L1 adipocytes was enhanced (IC50 = 582.91 ± 3.0 µg/mL) but lower than metformin (IC50 = 99.58 ± 0.59 µg/mL). These results highlight P. djamor var. fuscopruinosus as a promising functional food with strong antioxidant, anticancer effects, and antidiabetic properties. Further studies should optimize cultivation, isolate bioactive compounds, assess safety, and validate therapeutic properties through in vivo studies.https://doi.org/10.1038/s41598-025-02900-4BioactivitiesEdible mushroomNutritionalTaxonomyPleurotoid |
| spellingShingle | Monthien Phonemany Phongeun Sysouphanthong Narawadee Rujanapun Satyajit D. Sarker Lutfun Nahar Panupong Puttarak Poonsit Hiransai Naritsada Thongklang Rawiwan Charoensup Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus Scientific Reports Bioactivities Edible mushroom Nutritional Taxonomy Pleurotoid |
| title | Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus |
| title_full | Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus |
| title_fullStr | Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus |
| title_full_unstemmed | Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus |
| title_short | Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus |
| title_sort | identification and therapeutic efficacy of pleurotus djamor var fuscopruinosus |
| topic | Bioactivities Edible mushroom Nutritional Taxonomy Pleurotoid |
| url | https://doi.org/10.1038/s41598-025-02900-4 |
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