Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring

Background: Oral Squamous Cell Carcinoma (OSCC) is a prevalent malignancy characterized by high morbidity and mortality. Chronic inflammation within the tumor microenvironment plays a pivotal role in OSCC progression, with CXCL8 or interleukin-8 (IL-8) emerging as a significant cytokine implicated i...

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Main Authors: Hema Shree K, Gayathri R, Vishnu Priya Veeraraghavan, Selvaraj J, Pratibha Ramani
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Oral Oncology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772906025000111
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author Hema Shree K
Gayathri R
Vishnu Priya Veeraraghavan
Selvaraj J
Pratibha Ramani
author_facet Hema Shree K
Gayathri R
Vishnu Priya Veeraraghavan
Selvaraj J
Pratibha Ramani
author_sort Hema Shree K
collection DOAJ
description Background: Oral Squamous Cell Carcinoma (OSCC) is a prevalent malignancy characterized by high morbidity and mortality. Chronic inflammation within the tumor microenvironment plays a pivotal role in OSCC progression, with CXCL8 or interleukin-8 (IL-8) emerging as a significant cytokine implicated in various pro-tumorigenic processes. IL-8 promotes angiogenesis, immune evasion, and cancer cell stemness, contributing to tumor aggression and resistance to therapy. Understanding longitudinal changes in IL-8 expression provides insights into OSCC progression and treatment responses, potentially guiding personalized therapeutic strategies. Objectives: To investigate longitudinal trends in IL-8 expression among OSCC patients. To assess correlations between IL-8 levels and disease progression, treatment outcomes, and patient survival. To evaluate IL-8's prognostic value as a biomarker and its potential as a therapeutic target. Methods: A prospective longitudinal study enrolled 75 OSCC patients divided into pre-treatment, post-treatment, and follow-up groups. Saliva samples were collected, and IL-8 expression levels were quantified using RT-PCR and ELISA. Temporal trends in IL-8 expression and associations with clinical outcomes were analyzed using mixed-effects models and Kaplan-Meier survival analysis. Results: Significant elevation of IL-8 levels was observed in OSCC patients compared to controls. IL-8 levels decreased post-treatment but remained elevated compared to controls, with higher levels correlating with advanced tumor stages and poorer differentiation. Kaplan-Meier analysis demonstrated reduced survival probabilities in patients with persistently high IL-8 expression. Conclusions: Longitudinal monitoring of IL-8 expression offers valuable insights into OSCC pathogenesis and treatment response. IL-8's role as a prognostic biomarker and therapeutic target warrants further exploration to enhance disease management and patient outcomes.
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spelling doaj-art-43a054acef8744d9917b985edc3ca6322025-08-20T03:42:44ZengElsevierOral Oncology Reports2772-90602025-03-011310072310.1016/j.oor.2025.100723Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoringHema Shree K0Gayathri R1Vishnu Priya Veeraraghavan2Selvaraj J3Pratibha Ramani4Department of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute Medical and Technical Science, Saveetha University, Chennai, IndiaDepartment of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute Medical and Technical Science, Saveetha University, Chennai, India; Corresponding author.Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute Medical and Technical Science, Saveetha University, Chennai, IndiaDepartment of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute Medical and Technical Science, Saveetha University, Chennai, IndiaDepartment of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute Medical and Technical Science, Saveetha University, Chennai, IndiaBackground: Oral Squamous Cell Carcinoma (OSCC) is a prevalent malignancy characterized by high morbidity and mortality. Chronic inflammation within the tumor microenvironment plays a pivotal role in OSCC progression, with CXCL8 or interleukin-8 (IL-8) emerging as a significant cytokine implicated in various pro-tumorigenic processes. IL-8 promotes angiogenesis, immune evasion, and cancer cell stemness, contributing to tumor aggression and resistance to therapy. Understanding longitudinal changes in IL-8 expression provides insights into OSCC progression and treatment responses, potentially guiding personalized therapeutic strategies. Objectives: To investigate longitudinal trends in IL-8 expression among OSCC patients. To assess correlations between IL-8 levels and disease progression, treatment outcomes, and patient survival. To evaluate IL-8's prognostic value as a biomarker and its potential as a therapeutic target. Methods: A prospective longitudinal study enrolled 75 OSCC patients divided into pre-treatment, post-treatment, and follow-up groups. Saliva samples were collected, and IL-8 expression levels were quantified using RT-PCR and ELISA. Temporal trends in IL-8 expression and associations with clinical outcomes were analyzed using mixed-effects models and Kaplan-Meier survival analysis. Results: Significant elevation of IL-8 levels was observed in OSCC patients compared to controls. IL-8 levels decreased post-treatment but remained elevated compared to controls, with higher levels correlating with advanced tumor stages and poorer differentiation. Kaplan-Meier analysis demonstrated reduced survival probabilities in patients with persistently high IL-8 expression. Conclusions: Longitudinal monitoring of IL-8 expression offers valuable insights into OSCC pathogenesis and treatment response. IL-8's role as a prognostic biomarker and therapeutic target warrants further exploration to enhance disease management and patient outcomes.http://www.sciencedirect.com/science/article/pii/S2772906025000111IL-8OSCCPrognostic biomarkerKaplan Meier analysisELISA
spellingShingle Hema Shree K
Gayathri R
Vishnu Priya Veeraraghavan
Selvaraj J
Pratibha Ramani
Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
Oral Oncology Reports
IL-8
OSCC
Prognostic biomarker
Kaplan Meier analysis
ELISA
title Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
title_full Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
title_fullStr Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
title_full_unstemmed Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
title_short Pro-inflammatory mediator and cancer-associated chemokine CXCL8 as a prognostic indicator of tumor aggressiveness, angiogenesis, and disease progression with focus on therapeutic implications and recurrence monitoring
title_sort pro inflammatory mediator and cancer associated chemokine cxcl8 as a prognostic indicator of tumor aggressiveness angiogenesis and disease progression with focus on therapeutic implications and recurrence monitoring
topic IL-8
OSCC
Prognostic biomarker
Kaplan Meier analysis
ELISA
url http://www.sciencedirect.com/science/article/pii/S2772906025000111
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