The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut
The mammalian intestine is a major site of colonization and a starting point of severe infections by Klebsiella pneumoniae. Inflammatory bowel disease (IBD) is an inflammatory disorder of the gut, and host-derived nitrate in IBD confers a luminal growth advantage upon Escherichia coli and Salmonella...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2474185 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850109285252464640 |
|---|---|
| author | Jichen Xie Hui Wang Renhui Ma Jinming Fan Qiuhang Quan Zhiqiang Zhang Moran Li Bei Li |
| author_facet | Jichen Xie Hui Wang Renhui Ma Jinming Fan Qiuhang Quan Zhiqiang Zhang Moran Li Bei Li |
| author_sort | Jichen Xie |
| collection | DOAJ |
| description | The mammalian intestine is a major site of colonization and a starting point of severe infections by Klebsiella pneumoniae. Inflammatory bowel disease (IBD) is an inflammatory disorder of the gut, and host-derived nitrate in IBD confers a luminal growth advantage upon Escherichia coli and Salmonella typhimurium through nitrate respiration in the inflamed gut. However, the impact of nitrate on the growth and pathogenicity of K. pneumoniae in this microenvironment is poorly understood. In this study, we used oral administration of dextran sodium sulphate to induce IBD in mouse models. We then analysed the colonization levels of K. pneumoniae wild-type (WT), the nitrate reductase gene mutant strains (ΔnarG, ΔnarZ and ΔnarGΔnarZ), and the molybdate uptake gene mutant strain (ΔmodA) in the inflamed intestinal tract. Results showed that the growth, intestinal colonization, and extraintestinal dissemination of K. pneumoniae were increased in the intestines of dextran sulphate sodium (DSS)-treated mice. Nitrate in the inflamed bowel conferred a growth advantage to K. pneumoniae through nitrate respiration. The molybdate transport protein ModA regulated nitrate reductase activity to increase the growth, intestinal colonization, and extraintestinal dissemination of K. pneumoniae. Tungstate will be a promising antibacterial agent to tackle K. pneumoniae infections in IBD patients. |
| format | Article |
| id | doaj-art-439fb1db2ea24d76b8f455e071ce9b13 |
| institution | OA Journals |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-439fb1db2ea24d76b8f455e071ce9b132025-08-20T02:38:06ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2474185The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gutJichen Xie0Hui Wang1Renhui Ma2Jinming Fan3Qiuhang Quan4Zhiqiang Zhang5Moran Li6Bei Li7School of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaSchool of Basic Medical Science, Hubei University of Medicine, Shiyan, ChinaThe mammalian intestine is a major site of colonization and a starting point of severe infections by Klebsiella pneumoniae. Inflammatory bowel disease (IBD) is an inflammatory disorder of the gut, and host-derived nitrate in IBD confers a luminal growth advantage upon Escherichia coli and Salmonella typhimurium through nitrate respiration in the inflamed gut. However, the impact of nitrate on the growth and pathogenicity of K. pneumoniae in this microenvironment is poorly understood. In this study, we used oral administration of dextran sodium sulphate to induce IBD in mouse models. We then analysed the colonization levels of K. pneumoniae wild-type (WT), the nitrate reductase gene mutant strains (ΔnarG, ΔnarZ and ΔnarGΔnarZ), and the molybdate uptake gene mutant strain (ΔmodA) in the inflamed intestinal tract. Results showed that the growth, intestinal colonization, and extraintestinal dissemination of K. pneumoniae were increased in the intestines of dextran sulphate sodium (DSS)-treated mice. Nitrate in the inflamed bowel conferred a growth advantage to K. pneumoniae through nitrate respiration. The molybdate transport protein ModA regulated nitrate reductase activity to increase the growth, intestinal colonization, and extraintestinal dissemination of K. pneumoniae. Tungstate will be a promising antibacterial agent to tackle K. pneumoniae infections in IBD patients.https://www.tandfonline.com/doi/10.1080/21505594.2025.2474185IBDKlebsiella pneumoniaeModAnitrate reductaseintestinal colonization |
| spellingShingle | Jichen Xie Hui Wang Renhui Ma Jinming Fan Qiuhang Quan Zhiqiang Zhang Moran Li Bei Li The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut Virulence IBD Klebsiella pneumoniae ModA nitrate reductase intestinal colonization |
| title | The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut |
| title_full | The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut |
| title_fullStr | The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut |
| title_full_unstemmed | The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut |
| title_short | The molybdate transport protein ModA regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of Klebsiella pneumoniae in the inflamed gut |
| title_sort | molybdate transport protein moda regulates nitrate reductase activity to increase the intestinal colonization and extraintestinal dissemination of klebsiella pneumoniae in the inflamed gut |
| topic | IBD Klebsiella pneumoniae ModA nitrate reductase intestinal colonization |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2474185 |
| work_keys_str_mv | AT jichenxie themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT huiwang themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT renhuima themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT jinmingfan themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT qiuhangquan themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT zhiqiangzhang themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT moranli themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT beili themolybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT jichenxie molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT huiwang molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT renhuima molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT jinmingfan molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT qiuhangquan molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT zhiqiangzhang molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT moranli molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut AT beili molybdatetransportproteinmodaregulatesnitratereductaseactivitytoincreasetheintestinalcolonizationandextraintestinaldisseminationofklebsiellapneumoniaeintheinflamedgut |