Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia

Familial hypercholesterolemia (FH) is a lipoprotein disorder characterized by elevated plasma levels of low-density lipoprotein cholesterol (LDL-C) and an increased risk of premature atherosclerotic cardiovascular disease. Recent evidences have shown that several glycerophospholipid species were mar...

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Main Authors: Zhiyong Du, Yu Wang, Fan Li, Xuechun Sun, Yunhui Du, Linyi Li, Huahui Yu, Chaowei Hu, Haili Sun, Xiaoqian Gao, Lijie Han, Zihan Zhang, Jingci Xing, Luya Wang, Jianping Li, Yanwen Qin
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS) 2025-01-01
Series:Research
Online Access:https://spj.science.org/doi/10.34133/research.0629
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_version_ 1849718530182742016
author Zhiyong Du
Yu Wang
Fan Li
Xuechun Sun
Yunhui Du
Linyi Li
Huahui Yu
Chaowei Hu
Haili Sun
Xiaoqian Gao
Lijie Han
Zihan Zhang
Jingci Xing
Luya Wang
Jianping Li
Yanwen Qin
author_facet Zhiyong Du
Yu Wang
Fan Li
Xuechun Sun
Yunhui Du
Linyi Li
Huahui Yu
Chaowei Hu
Haili Sun
Xiaoqian Gao
Lijie Han
Zihan Zhang
Jingci Xing
Luya Wang
Jianping Li
Yanwen Qin
author_sort Zhiyong Du
collection DOAJ
description Familial hypercholesterolemia (FH) is a lipoprotein disorder characterized by elevated plasma levels of low-density lipoprotein cholesterol (LDL-C) and an increased risk of premature atherosclerotic cardiovascular disease. Recent evidences have shown that several glycerophospholipid species were markedly altered in experimental FH animals and exhibited diverse bioactivities. Nevertheless, the glycerophospholipid profiles and their associated biological implications in human FH remain largely unknown. In this study, we sought to comprehensively delineate the glycerophospholipid phenotypes in human FH and to investigate the functional roles of key FH-altered glycerophospholipid molecules on cholesterol metabolism. Targeted analysis of 328 glycerophospholipid metabolites was used to profile the differentiated alterations in patients with homozygous FH (HoFH; n = 181), heterozygous FH (HeFH; n = 452), and non-FH hypercholesterolemia (n = 382). Our findings revealed that the glycerophospholipid phenotypes of FH and non-FH hypercholesterolemia were dominated by a spectrum of metabolites involved in the lysophosphatidic acid (LPA) metabolism. Among the LPA features, palmitoyl-LPA (16:0) showed significant association with the clinical levels of LDL-C and total cholesterol in HoFH and HeFH populations. Using functional metabolomic strategy and murine FH model, we demonstrated that supplementation with LPA 16:0 elevated the plasma levels of LDL and free/esterified cholesterol and exacerbated the atherosclerotic lesions. Conversely, inhibition of autotaxin-mediated LPA 16:0 production significantly ameliorated dyslipidemia. Mechanistically, we uncovered that LPA 16:0 could disrupt hepatic cholesterol homeostasis by impairing cholesterol excretion and inhibiting primary bile acid synthesis. In summary, our study offers novel insights into lipid metabolism in human FH and posits that targeting LPA metabolism may represent a promising therapeutic strategy for reducing cholesterol levels in the FH population.
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spelling doaj-art-4399cbb7a346485d9e470210c32b367e2025-08-20T03:12:20ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742025-01-01810.34133/research.0629Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial HypercholesterolemiaZhiyong Du0Yu Wang1Fan Li2Xuechun Sun3Yunhui Du4Linyi Li5Huahui Yu6Chaowei Hu7Haili Sun8Xiaoqian Gao9Lijie Han10Zihan Zhang11Jingci Xing12Luya Wang13Jianping Li14Yanwen Qin15Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Department of Cardiology, Peking University First Hospital, Beijing 100034, China.Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing 100029, China.Familial hypercholesterolemia (FH) is a lipoprotein disorder characterized by elevated plasma levels of low-density lipoprotein cholesterol (LDL-C) and an increased risk of premature atherosclerotic cardiovascular disease. Recent evidences have shown that several glycerophospholipid species were markedly altered in experimental FH animals and exhibited diverse bioactivities. Nevertheless, the glycerophospholipid profiles and their associated biological implications in human FH remain largely unknown. In this study, we sought to comprehensively delineate the glycerophospholipid phenotypes in human FH and to investigate the functional roles of key FH-altered glycerophospholipid molecules on cholesterol metabolism. Targeted analysis of 328 glycerophospholipid metabolites was used to profile the differentiated alterations in patients with homozygous FH (HoFH; n = 181), heterozygous FH (HeFH; n = 452), and non-FH hypercholesterolemia (n = 382). Our findings revealed that the glycerophospholipid phenotypes of FH and non-FH hypercholesterolemia were dominated by a spectrum of metabolites involved in the lysophosphatidic acid (LPA) metabolism. Among the LPA features, palmitoyl-LPA (16:0) showed significant association with the clinical levels of LDL-C and total cholesterol in HoFH and HeFH populations. Using functional metabolomic strategy and murine FH model, we demonstrated that supplementation with LPA 16:0 elevated the plasma levels of LDL and free/esterified cholesterol and exacerbated the atherosclerotic lesions. Conversely, inhibition of autotaxin-mediated LPA 16:0 production significantly ameliorated dyslipidemia. Mechanistically, we uncovered that LPA 16:0 could disrupt hepatic cholesterol homeostasis by impairing cholesterol excretion and inhibiting primary bile acid synthesis. In summary, our study offers novel insights into lipid metabolism in human FH and posits that targeting LPA metabolism may represent a promising therapeutic strategy for reducing cholesterol levels in the FH population.https://spj.science.org/doi/10.34133/research.0629
spellingShingle Zhiyong Du
Yu Wang
Fan Li
Xuechun Sun
Yunhui Du
Linyi Li
Huahui Yu
Chaowei Hu
Haili Sun
Xiaoqian Gao
Lijie Han
Zihan Zhang
Jingci Xing
Luya Wang
Jianping Li
Yanwen Qin
Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
Research
title Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
title_full Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
title_fullStr Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
title_full_unstemmed Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
title_short Targeting Lysophosphatidic Acid Ameliorates Dyslipidemia in Familial Hypercholesterolemia
title_sort targeting lysophosphatidic acid ameliorates dyslipidemia in familial hypercholesterolemia
url https://spj.science.org/doi/10.34133/research.0629
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