Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin
The rapid and reliable detection of pathogenic bacteria remains a significant challenge in clinical microbiology. Consequently, the demand for simple and rapid techniques, such as antimicrobial peptide (AMP)-based sensors, has recently increased as an alternative to traditional methods. Melittin, a...
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| Language: | English |
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/1/12 |
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| author | Sun-Mi Lee Jun-Ho Song Kyo-Seok Lee Kyung-Hwa Yoo |
| author_facet | Sun-Mi Lee Jun-Ho Song Kyo-Seok Lee Kyung-Hwa Yoo |
| author_sort | Sun-Mi Lee |
| collection | DOAJ |
| description | The rapid and reliable detection of pathogenic bacteria remains a significant challenge in clinical microbiology. Consequently, the demand for simple and rapid techniques, such as antimicrobial peptide (AMP)-based sensors, has recently increased as an alternative to traditional methods. Melittin, a broad-spectrum AMP, rapidly associates with the cell membranes of various gram-positive and gram-negative bacteria. It also inhibits bacterial biofilm formation in blood culture media. In our study, bacterial growth was measured using electrical vertical-capacitance sensors with interdigitated electrodes functionalized with melittin, a widely studied AMP. The melittin-immobilized vertical-capacitance sensors demonstrated real-time detection of both standard and clinically isolated bacteria in media. Furthermore, these sensors successfully detected clinically isolated bacteria in blood culture media while inhibiting bacterial biofilm formation. Melittin-immobilized vertical-capacitance sensors provide a rapid and sensitive pathogen detection platform, with significant potential for improving patient care. |
| format | Article |
| id | doaj-art-438e6581321f48bc9a5743323bcd1998 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-438e6581321f48bc9a5743323bcd19982025-01-10T13:20:31ZengMDPI AGSensors1424-82202024-12-012511210.3390/s25010012Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide MelittinSun-Mi Lee0Jun-Ho Song1Kyo-Seok Lee2Kyung-Hwa Yoo3Department of Physics, Yonsei University, Seoul 03722, Republic of KoreaDepartment of Physics, Yonsei University, Seoul 03722, Republic of KoreaDepartment of Physics, Yonsei University, Seoul 03722, Republic of KoreaDepartment of Physics, Yonsei University, Seoul 03722, Republic of KoreaThe rapid and reliable detection of pathogenic bacteria remains a significant challenge in clinical microbiology. Consequently, the demand for simple and rapid techniques, such as antimicrobial peptide (AMP)-based sensors, has recently increased as an alternative to traditional methods. Melittin, a broad-spectrum AMP, rapidly associates with the cell membranes of various gram-positive and gram-negative bacteria. It also inhibits bacterial biofilm formation in blood culture media. In our study, bacterial growth was measured using electrical vertical-capacitance sensors with interdigitated electrodes functionalized with melittin, a widely studied AMP. The melittin-immobilized vertical-capacitance sensors demonstrated real-time detection of both standard and clinically isolated bacteria in media. Furthermore, these sensors successfully detected clinically isolated bacteria in blood culture media while inhibiting bacterial biofilm formation. Melittin-immobilized vertical-capacitance sensors provide a rapid and sensitive pathogen detection platform, with significant potential for improving patient care.https://www.mdpi.com/1424-8220/25/1/12antimicrobial peptidesmelittinclinical bacteria detectionvertical-capacitance sensoranti-biofilm formation |
| spellingShingle | Sun-Mi Lee Jun-Ho Song Kyo-Seok Lee Kyung-Hwa Yoo Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin Sensors antimicrobial peptides melittin clinical bacteria detection vertical-capacitance sensor anti-biofilm formation |
| title | Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin |
| title_full | Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin |
| title_fullStr | Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin |
| title_full_unstemmed | Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin |
| title_short | Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin |
| title_sort | pathogenic bacterial detection using vertical capacitance sensor array immobilized with the antimicrobial peptide melittin |
| topic | antimicrobial peptides melittin clinical bacteria detection vertical-capacitance sensor anti-biofilm formation |
| url | https://www.mdpi.com/1424-8220/25/1/12 |
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