The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis
Abstract Recently, interest in investigating the effects of long noncoding RNAs (lncRNAs) on endometrial receptivity (ER) has increased within the field of assisted reproductive technology. Therefore, the objective of this study is to identify and analyze the role of the lncRNA LUCAT1 and to elucida...
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07718-4 |
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| author | Junyu Shang Yumei Chen Qianwen Jiang Wenxin Li Minjun Lu Jiamin Zhou Li Lin Jie Xing Mengxue Zhang Shijie Zhao Jingjing Lu Xuyan Shi Yueqin Liu Xiaolan Zhu |
| author_facet | Junyu Shang Yumei Chen Qianwen Jiang Wenxin Li Minjun Lu Jiamin Zhou Li Lin Jie Xing Mengxue Zhang Shijie Zhao Jingjing Lu Xuyan Shi Yueqin Liu Xiaolan Zhu |
| author_sort | Junyu Shang |
| collection | DOAJ |
| description | Abstract Recently, interest in investigating the effects of long noncoding RNAs (lncRNAs) on endometrial receptivity (ER) has increased within the field of assisted reproductive technology. Therefore, the objective of this study is to identify and analyze the role of the lncRNA LUCAT1 and to elucidate its specific mechanism in regulating ER. Hub genes associated with ER are identified via Weighted gene co-expression network analysis (WGCNA) in two datasets downloaded from the GEO database. These hub genes identified via WGCNA were subsequently validated. The combination of a dual-luciferase assay, qRT‒PCR, western blotting, and other techniques are used to investigate the molecular mechanism by which LUCAT1 regulates S100P. In this study, LUCAT1 expression is shown to significantly affect ER, and the depletion of LUCAT1 leads to impaired ER function. Additionally, LUCAT1 is shown to act as a molecular sponge for miR-495-3p, thereby modulating the expression of S100P. This modulation influences the proliferation, migration, and invasion capabilities of Ishikawa cells, as well as the adhesion of JAR cells to endometrial cells. Therefore, LUCAT1 can regulate ER via the miR-495-3p/S100P axis, which provides experimental evidence for the identification of innovative strategies aimed at enhancing endometrial receptivity. |
| format | Article |
| id | doaj-art-437d12383aad4f04af71497dfbff8a86 |
| institution | DOAJ |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Communications Biology |
| spelling | doaj-art-437d12383aad4f04af71497dfbff8a862025-08-20T03:03:40ZengNature PortfolioCommunications Biology2399-36422025-02-018111310.1038/s42003-025-07718-4The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axisJunyu Shang0Yumei Chen1Qianwen Jiang2Wenxin Li3Minjun Lu4Jiamin Zhou5Li Lin6Jie Xing7Mengxue Zhang8Shijie Zhao9Jingjing Lu10Xuyan Shi11Yueqin Liu12Xiaolan Zhu13Reproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityDepartment of Central Laboratory, The Fourth Affiliated Hospital of Jiangsu UniversityCollege of Pharmacy, Guangxi UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityReproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu UniversityAbstract Recently, interest in investigating the effects of long noncoding RNAs (lncRNAs) on endometrial receptivity (ER) has increased within the field of assisted reproductive technology. Therefore, the objective of this study is to identify and analyze the role of the lncRNA LUCAT1 and to elucidate its specific mechanism in regulating ER. Hub genes associated with ER are identified via Weighted gene co-expression network analysis (WGCNA) in two datasets downloaded from the GEO database. These hub genes identified via WGCNA were subsequently validated. The combination of a dual-luciferase assay, qRT‒PCR, western blotting, and other techniques are used to investigate the molecular mechanism by which LUCAT1 regulates S100P. In this study, LUCAT1 expression is shown to significantly affect ER, and the depletion of LUCAT1 leads to impaired ER function. Additionally, LUCAT1 is shown to act as a molecular sponge for miR-495-3p, thereby modulating the expression of S100P. This modulation influences the proliferation, migration, and invasion capabilities of Ishikawa cells, as well as the adhesion of JAR cells to endometrial cells. Therefore, LUCAT1 can regulate ER via the miR-495-3p/S100P axis, which provides experimental evidence for the identification of innovative strategies aimed at enhancing endometrial receptivity.https://doi.org/10.1038/s42003-025-07718-4 |
| spellingShingle | Junyu Shang Yumei Chen Qianwen Jiang Wenxin Li Minjun Lu Jiamin Zhou Li Lin Jie Xing Mengxue Zhang Shijie Zhao Jingjing Lu Xuyan Shi Yueqin Liu Xiaolan Zhu The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis Communications Biology |
| title | The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis |
| title_full | The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis |
| title_fullStr | The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis |
| title_full_unstemmed | The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis |
| title_short | The long noncoding RNA LUCAT1 regulates endometrial receptivity via the miR-495-3p/S100P axis |
| title_sort | long noncoding rna lucat1 regulates endometrial receptivity via the mir 495 3p s100p axis |
| url | https://doi.org/10.1038/s42003-025-07718-4 |
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