Plant stem cell maintenance involves direct transcriptional repression of differentiation program
Abstract In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of st...
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| Format: | Article |
| Language: | English |
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Springer Nature
2013-04-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2013.8 |
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| _version_ | 1849331379188269056 |
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| author | Ram Kishor Yadav Mariano Perales Jérémy Gruel Carolyn Ohno Marcus Heisler Thomas Girke Henrik Jönsson G Venugopala Reddy |
| author_facet | Ram Kishor Yadav Mariano Perales Jérémy Gruel Carolyn Ohno Marcus Heisler Thomas Girke Henrik Jönsson G Venugopala Reddy |
| author_sort | Ram Kishor Yadav |
| collection | DOAJ |
| description | Abstract In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high‐resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of differentiation promoting TFs; KANADI1, KANADI2, ASYMMETRICLEAVES2 and YABBY3 to repress their expression. Predictions from a computational model, supported by live imaging, reveal that WUS‐mediated repression prevents premature differentiation of stem cell progenitors, being part of a minimal regulatory network for meristem maintenance. Our work shows that direct transcriptional repression of differentiation promoting TFs is an evolutionarily conserved logic for stem cell regulation. |
| format | Article |
| id | doaj-art-43707dc6e24e466ca88d5cda388af39a |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2013-04-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-43707dc6e24e466ca88d5cda388af39a2025-08-20T03:46:37ZengSpringer NatureMolecular Systems Biology1744-42922013-04-019111310.1038/msb.2013.8Plant stem cell maintenance involves direct transcriptional repression of differentiation programRam Kishor Yadav0Mariano Perales1Jérémy Gruel2Carolyn Ohno3Marcus Heisler4Thomas Girke5Henrik Jönsson6G Venugopala Reddy7Department of Botany and Plant Sciences, Center for Plant Cell Biology (CEPCEB), Institute of Integrative Genome Biology (IIGB), University of CaliforniaDepartment of Botany and Plant Sciences, Center for Plant Cell Biology (CEPCEB), Institute of Integrative Genome Biology (IIGB), University of CaliforniaComputational Biology and Biological Physics Group, Department of Astronomy and Theoretical Physics, Lund UniversityEuropean Molecular Biology LaboratoryEuropean Molecular Biology LaboratoryDepartment of Botany and Plant Sciences, Center for Plant Cell Biology (CEPCEB), Institute of Integrative Genome Biology (IIGB), University of CaliforniaComputational Biology and Biological Physics Group, Department of Astronomy and Theoretical Physics, Lund UniversityDepartment of Botany and Plant Sciences, Center for Plant Cell Biology (CEPCEB), Institute of Integrative Genome Biology (IIGB), University of CaliforniaAbstract In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high‐resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of differentiation promoting TFs; KANADI1, KANADI2, ASYMMETRICLEAVES2 and YABBY3 to repress their expression. Predictions from a computational model, supported by live imaging, reveal that WUS‐mediated repression prevents premature differentiation of stem cell progenitors, being part of a minimal regulatory network for meristem maintenance. Our work shows that direct transcriptional repression of differentiation promoting TFs is an evolutionarily conserved logic for stem cell regulation.https://doi.org/10.1038/msb.2013.8central zoneCLAVATA3shoot apical meristemstem cell nicheWUSCHEL |
| spellingShingle | Ram Kishor Yadav Mariano Perales Jérémy Gruel Carolyn Ohno Marcus Heisler Thomas Girke Henrik Jönsson G Venugopala Reddy Plant stem cell maintenance involves direct transcriptional repression of differentiation program Molecular Systems Biology central zone CLAVATA3 shoot apical meristem stem cell niche WUSCHEL |
| title | Plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| title_full | Plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| title_fullStr | Plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| title_full_unstemmed | Plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| title_short | Plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| title_sort | plant stem cell maintenance involves direct transcriptional repression of differentiation program |
| topic | central zone CLAVATA3 shoot apical meristem stem cell niche WUSCHEL |
| url | https://doi.org/10.1038/msb.2013.8 |
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