Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection
The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multipar...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/2909487 |
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| author | Guadalupe Rivera-Torruco Carolina A. Martínez-Mendiola Tania Angeles-Floriano Gustavo Alberto Jaimes-Ortega José Luis Maravillas-Montero Rodolfo García-Contreras Yolanda González Esmeralda Juárez Porfirio Nava Vianney Ortiz-Navarrete Oscar Medina-Contreras Paula Licona-Limón Ricardo Valle-Rios |
| author_facet | Guadalupe Rivera-Torruco Carolina A. Martínez-Mendiola Tania Angeles-Floriano Gustavo Alberto Jaimes-Ortega José Luis Maravillas-Montero Rodolfo García-Contreras Yolanda González Esmeralda Juárez Porfirio Nava Vianney Ortiz-Navarrete Oscar Medina-Contreras Paula Licona-Limón Ricardo Valle-Rios |
| author_sort | Guadalupe Rivera-Torruco |
| collection | DOAJ |
| description | The process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1+ Lineage- Sca-1+ c-kit+ (ISM1+ LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1+ ISM1+ cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1+ cells are lung-resident cells. We also observed that ISM1+ cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1+LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1+ cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis. |
| format | Article |
| id | doaj-art-4362988b872c4b4b8b4b3675ccad282f |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4362988b872c4b4b8b4b3675ccad282f2025-02-03T06:01:09ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/2909487Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during InfectionGuadalupe Rivera-Torruco0Carolina A. Martínez-Mendiola1Tania Angeles-Floriano2Gustavo Alberto Jaimes-Ortega3José Luis Maravillas-Montero4Rodolfo García-Contreras5Yolanda González6Esmeralda Juárez7Porfirio Nava8Vianney Ortiz-Navarrete9Oscar Medina-Contreras10Paula Licona-Limón11Ricardo Valle-Rios12Unidad Universitaria de InvestigaciónUnidad Universitaria de InvestigaciónUnidad Universitaria de InvestigaciónLaboratorio de Investigación en Inmunología y ProteómicaRed de Apoyo a la InvestigaciónDepartamento de Microbiología y ParasitologíaDepartamento de Investigación en MicrobiologíaDepartamento de Investigación en MicrobiologíaDepartamento de FisiologíaDepartamento de Biomedicina MolecularUnidad de Investigación Epidemiológica en Endocrinología y NutriciónDepartamento de Biología Celular y del DesarrolloUnidad Universitaria de InvestigaciónThe process by which blood cells are generated has been widely studied in homeostasis and during pathogen-triggered inflammatory response. Recently, murine lungs have been shown to be a significant source of hematopoietic progenitors in a process known as extramedullary hematopoiesis. Using multiparametric flow cytometry, we have identified mesenchymal, endothelial, and hematopoietic progenitor cells that express the secreted small protein Isthmin 1 (ISM1). Further characterization of hematopoietic progenitor cells indicated that ISM1+ Lineage- Sca-1+ c-kit+ (ISM1+ LSK) cells are enriched in short-term hematopoietic stem cells (ST-HSCs). Moreover, most Sca-1+ ISM1+ cells express the residence marker CD49a, and this correlated with their localization in the extravascular region of the lung, indicating that ISM1+ cells are lung-resident cells. We also observed that ISM1+ cells express TLR4, TLR5, and TLR9, and, in a mouse model of sepsis induced by P. aeruginosa, we observed that all the LSK and ISM1+LSK cells were affected. We conclude that ISM1 is a novel biomarker associated with progenitor-like cells. ISM1+ cells are involved in the response to a bacterial challenge, suggesting an association between ISM1-producing cells and dangerous inflammatory responses like sepsis.http://dx.doi.org/10.1155/2022/2909487 |
| spellingShingle | Guadalupe Rivera-Torruco Carolina A. Martínez-Mendiola Tania Angeles-Floriano Gustavo Alberto Jaimes-Ortega José Luis Maravillas-Montero Rodolfo García-Contreras Yolanda González Esmeralda Juárez Porfirio Nava Vianney Ortiz-Navarrete Oscar Medina-Contreras Paula Licona-Limón Ricardo Valle-Rios Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection Journal of Immunology Research |
| title | Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection |
| title_full | Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection |
| title_fullStr | Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection |
| title_full_unstemmed | Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection |
| title_short | Isthmin 1 is Expressed by Progenitor-Like Cells in the Lung: Phenotypical Analysis of Isthmin 1+ Hematopoietic Stem-Like Cells in Homeostasis and during Infection |
| title_sort | isthmin 1 is expressed by progenitor like cells in the lung phenotypical analysis of isthmin 1 hematopoietic stem like cells in homeostasis and during infection |
| url | http://dx.doi.org/10.1155/2022/2909487 |
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