Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy
This study characterized the level of oxidative metabolism in skeletal muscle during whole-body activity as a percentage of the muscle’s maximum oxidative rate (mVO<sub>2</sub>max) using near-infrared spectroscopy (NIRS). Ten healthy participants completed a progressive work test and who...
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MDPI AG
2024-10-01
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| author | Kevin K. McCully Sarah N. Stoddard Mary Ann Reynolds Terence E. Ryan |
| author_facet | Kevin K. McCully Sarah N. Stoddard Mary Ann Reynolds Terence E. Ryan |
| author_sort | Kevin K. McCully |
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| description | This study characterized the level of oxidative metabolism in skeletal muscle during whole-body activity as a percentage of the muscle’s maximum oxidative rate (mVO<sub>2</sub>max) using near-infrared spectroscopy (NIRS). Ten healthy participants completed a progressive work test and whole-body walking and lunge exercises, while oxygen saturation was collected from the vastus lateralis muscle using near-infrared spectroscopy (NIRS). Muscle oxygen consumption (mVO<sub>2</sub>) was determined using arterial occlusions following each exercise. mVO<sub>2</sub>max was extrapolated from the mVO<sub>2</sub> values determined from the progressive exercise test. mVO<sub>2</sub>max was 11.3 ± 3.3%/s on day one and 12.0 ± 2.9%/s on day two (<i>p</i> = 0.07). mVO<sub>2</sub>max had similar variation (ICC = 0.95, CV = 6.4%) to NIRS measures of oxidative metabolism. There was a progressive increase in mVO<sub>2</sub> with walking at 3.2 Km/h, 4.8 km/h, 6.4 Km/h, and with lunges (15.8 ± 6.6%, 20.5 ± 7.2%, 26.0 ± 6.6%, and 57.4 ± 15.4% of mVO<sub>2</sub>max, respectively). Lunges showed a high reliability (ICC = 0.81, CV = 10.2%). Muscle oxidative metabolism in response to whole-body exercise can be reproducibly measured with arterial occlusions and NIRS. This method may be used to further research on mitochondrial activation within a single muscle during whole-body exercise. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-434f3f9260c343d3ae093b1927eb32562025-01-24T13:44:20ZengMDPI AGNDT2813-477X2024-10-012441742910.3390/ndt2040025Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared SpectroscopyKevin K. McCully0Sarah N. Stoddard1Mary Ann Reynolds2Terence E. Ryan3Noninvasive Exercise Physiology Laboratory, Department of Kinesiology, University of Georgia, Athens, GA 30602, USANoninvasive Exercise Physiology Laboratory, Department of Kinesiology, University of Georgia, Athens, GA 30602, USANoninvasive Exercise Physiology Laboratory, Department of Kinesiology, University of Georgia, Athens, GA 30602, USAMolecular Metabolism Laboratory, Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USAThis study characterized the level of oxidative metabolism in skeletal muscle during whole-body activity as a percentage of the muscle’s maximum oxidative rate (mVO<sub>2</sub>max) using near-infrared spectroscopy (NIRS). Ten healthy participants completed a progressive work test and whole-body walking and lunge exercises, while oxygen saturation was collected from the vastus lateralis muscle using near-infrared spectroscopy (NIRS). Muscle oxygen consumption (mVO<sub>2</sub>) was determined using arterial occlusions following each exercise. mVO<sub>2</sub>max was extrapolated from the mVO<sub>2</sub> values determined from the progressive exercise test. mVO<sub>2</sub>max was 11.3 ± 3.3%/s on day one and 12.0 ± 2.9%/s on day two (<i>p</i> = 0.07). mVO<sub>2</sub>max had similar variation (ICC = 0.95, CV = 6.4%) to NIRS measures of oxidative metabolism. There was a progressive increase in mVO<sub>2</sub> with walking at 3.2 Km/h, 4.8 km/h, 6.4 Km/h, and with lunges (15.8 ± 6.6%, 20.5 ± 7.2%, 26.0 ± 6.6%, and 57.4 ± 15.4% of mVO<sub>2</sub>max, respectively). Lunges showed a high reliability (ICC = 0.81, CV = 10.2%). Muscle oxidative metabolism in response to whole-body exercise can be reproducibly measured with arterial occlusions and NIRS. This method may be used to further research on mitochondrial activation within a single muscle during whole-body exercise.https://www.mdpi.com/2813-477X/2/4/25near-infrared spectroscopymetabolic ratewalkingmaximal oxidative capacity |
| spellingShingle | Kevin K. McCully Sarah N. Stoddard Mary Ann Reynolds Terence E. Ryan Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy NDT near-infrared spectroscopy metabolic rate walking maximal oxidative capacity |
| title | Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy |
| title_full | Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy |
| title_fullStr | Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy |
| title_full_unstemmed | Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy |
| title_short | Skeletal Muscle Oxidative Metabolism during Exercise Measured with Near Infrared Spectroscopy |
| title_sort | skeletal muscle oxidative metabolism during exercise measured with near infrared spectroscopy |
| topic | near-infrared spectroscopy metabolic rate walking maximal oxidative capacity |
| url | https://www.mdpi.com/2813-477X/2/4/25 |
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