Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles

Amoxicillin polybutylcyanoacrylate nanoparticles (AMX-PBCA-NP) were prepared by the emulsion polymerization method with butylcyanoacrylate (BCA) as a carrier material. The preparation procedure was optimized according to the results of single factor experiments and uniform design tests, and then the...

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Main Authors: YANG Xue-feng, OUYANG Wu-qing, LIU Yu-mei, ZHANG Wen-juan, SUN Jiang-cai
Format: Article
Language:English
Published: Zhejiang University Press 2009-03-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.012
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author YANG Xue-feng
OUYANG Wu-qing
LIU Yu-mei
ZHANG Wen-juan
SUN Jiang-cai
author_facet YANG Xue-feng
OUYANG Wu-qing
LIU Yu-mei
ZHANG Wen-juan
SUN Jiang-cai
author_sort YANG Xue-feng
collection DOAJ
description Amoxicillin polybutylcyanoacrylate nanoparticles (AMX-PBCA-NP) were prepared by the emulsion polymerization method with butylcyanoacrylate (BCA) as a carrier material. The preparation procedure was optimized according to the results of single factor experiments and uniform design tests, and then the quality of AMX-PBCA-NP was evaluated. The results show that the screened optimum conditions for the preparation of AMX-PBCA-NP were 4 g·L<sup>-1</sup> amoxicillin, 10 mL·L<sup>-1</sup> BCA, 10 g·L<sup>-1</sup> pluronic F68, 10 g·L<sup>-1</sup> dextran-70 and pH 3.0. The prepared nanoparticles were globular, unconglutinate, well-distributed. The mean diameter of the particles was (222.33±1.53) nm, polydispersity index was 0.03±0.02, the mean encapsulation ratio and drug-load was (73.47±0.96)% and (29.39±0.38)%, respectively. The release characteristics in vitro were consistent with two phases kinetics rule. The minimal inhibitory concentration (MIC) against E. coli ATCC<sub>25922</sub> and S. aureus ATCC<sub>25923</sub> was 1/2 times of the MIC of active pharmaceutical ingredients, respectively. The results above suggested that the optimal preparation procedure of AMX-PBCA-NP is practicable, AMX-PBCA-NP has the slow-release character and the antibacterial activity of amoxicillin is enhanced in vitro.
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spelling doaj-art-4341b149798540cca442fef89e0cc3ef2025-08-20T02:47:40ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-03-013519520010.3785/j.issn.1008-9209.2009.02.01210089209Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticlesYANG Xue-fengOUYANG Wu-qingLIU Yu-meiZHANG Wen-juanSUN Jiang-caiAmoxicillin polybutylcyanoacrylate nanoparticles (AMX-PBCA-NP) were prepared by the emulsion polymerization method with butylcyanoacrylate (BCA) as a carrier material. The preparation procedure was optimized according to the results of single factor experiments and uniform design tests, and then the quality of AMX-PBCA-NP was evaluated. The results show that the screened optimum conditions for the preparation of AMX-PBCA-NP were 4 g·L<sup>-1</sup> amoxicillin, 10 mL·L<sup>-1</sup> BCA, 10 g·L<sup>-1</sup> pluronic F68, 10 g·L<sup>-1</sup> dextran-70 and pH 3.0. The prepared nanoparticles were globular, unconglutinate, well-distributed. The mean diameter of the particles was (222.33±1.53) nm, polydispersity index was 0.03±0.02, the mean encapsulation ratio and drug-load was (73.47±0.96)% and (29.39±0.38)%, respectively. The release characteristics in vitro were consistent with two phases kinetics rule. The minimal inhibitory concentration (MIC) against E. coli ATCC<sub>25922</sub> and S. aureus ATCC<sub>25923</sub> was 1/2 times of the MIC of active pharmaceutical ingredients, respectively. The results above suggested that the optimal preparation procedure of AMX-PBCA-NP is practicable, AMX-PBCA-NP has the slow-release character and the antibacterial activity of amoxicillin is enhanced in vitro.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.012amoxicillinnanoparticlespolybutylcyanoacrylateuniform designquality evaluation
spellingShingle YANG Xue-feng
OUYANG Wu-qing
LIU Yu-mei
ZHANG Wen-juan
SUN Jiang-cai
Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
浙江大学学报. 农业与生命科学版
amoxicillin
nanoparticles
polybutylcyanoacrylate
uniform design
quality evaluation
title Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
title_full Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
title_fullStr Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
title_full_unstemmed Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
title_short Preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
title_sort preparation and quality evaluation of amoxicillin polybutylcyanoacrylate nanoparticles
topic amoxicillin
nanoparticles
polybutylcyanoacrylate
uniform design
quality evaluation
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.012
work_keys_str_mv AT yangxuefeng preparationandqualityevaluationofamoxicillinpolybutylcyanoacrylatenanoparticles
AT ouyangwuqing preparationandqualityevaluationofamoxicillinpolybutylcyanoacrylatenanoparticles
AT liuyumei preparationandqualityevaluationofamoxicillinpolybutylcyanoacrylatenanoparticles
AT zhangwenjuan preparationandqualityevaluationofamoxicillinpolybutylcyanoacrylatenanoparticles
AT sunjiangcai preparationandqualityevaluationofamoxicillinpolybutylcyanoacrylatenanoparticles