Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria
In this paper, we have analyzed changes in the proteomic spectrum of pea Pisum sativum L. roots during inoculation with rhizobial bacteria with the aim of revealing new regulators of symbiosis development. To study the changes in the proteome spectrum of pea roots, a differential twodimensional (2-D...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-04-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/1441 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575148784353280 |
|---|---|
| author | I. V. Leppyanen A. N. Kirienko A. A. Lobov E. A. Dolgikh |
| author_facet | I. V. Leppyanen A. N. Kirienko A. A. Lobov E. A. Dolgikh |
| author_sort | I. V. Leppyanen |
| collection | DOAJ |
| description | In this paper, we have analyzed changes in the proteomic spectrum of pea Pisum sativum L. roots during inoculation with rhizobial bacteria with the aim of revealing new regulators of symbiosis development. To study the changes in the proteome spectrum of pea roots, a differential twodimensional (2-D) electrophoresis was performed using fluorescent labels Cy2 and Cy5. The images obtained made it possible to identify differences between the control variant (uninoculated roots) and the root variant after inoculation with Rhizobium leguminosarum bv. viciae RCAM 1026 (24 hours after treatment). 20 proteins were revealed and identified, the synthesis of which was enhanced during the inoculation of pea roots by nodule bacteria. To identify the proteins, a mass spectrometric analysis of tryptic peptides was performed on a quadrupole-time-of-flight mass spectrometer combined with a high-performance liquid chromatograph. Among such proteins, the beta-subunit of the G protein and the disulfide isomerase/phospholipase C were first found, whose function can be related to the signal regulation of symbiosis. This indicates that G-proteins and phospholipases can play a key role in the development of early stages of symbiosis in peas. Further experiments are expected to show whether the beta-subunit of the G protein interacts with the receptors to Nod factors, and how this affects the further signaling. Other proteins that might be interesting were annexin D8 and D1, protein kinase interacting with calcinerin B, actin-binding protein profilin, GTP-binding protein Ran1. They may be involved in the regulation of reactions with calcium, the reorganization of the actin cytoskeleton and other important processes in plants. The study of the role of such regulatory proteins will later become the basis for understanding the complex system of signal regulation, which is activated in pea plants by interaction with nodule bacteria. |
| format | Article |
| id | doaj-art-433fcc429ccd4a32b9e4afc1275b10a5 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-433fcc429ccd4a32b9e4afc1275b10a52025-02-01T09:58:05ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-04-0122219620410.18699/VJ18.347740Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteriaI. V. Leppyanen0A. N. Kirienko1A. A. Lobov2E. A. Dolgikh3All-Russian Scientific Research Institute of Agricultural MicrobiologyAll-Russian Scientific Research Institute of Agricultural MicrobiologyResource Center “Development of Molecular and Cellular Technologies”, Science Park, St. Petersburg State UniversityAll-Russian Scientific Research Institute of Agricultural MicrobiologyIn this paper, we have analyzed changes in the proteomic spectrum of pea Pisum sativum L. roots during inoculation with rhizobial bacteria with the aim of revealing new regulators of symbiosis development. To study the changes in the proteome spectrum of pea roots, a differential twodimensional (2-D) electrophoresis was performed using fluorescent labels Cy2 and Cy5. The images obtained made it possible to identify differences between the control variant (uninoculated roots) and the root variant after inoculation with Rhizobium leguminosarum bv. viciae RCAM 1026 (24 hours after treatment). 20 proteins were revealed and identified, the synthesis of which was enhanced during the inoculation of pea roots by nodule bacteria. To identify the proteins, a mass spectrometric analysis of tryptic peptides was performed on a quadrupole-time-of-flight mass spectrometer combined with a high-performance liquid chromatograph. Among such proteins, the beta-subunit of the G protein and the disulfide isomerase/phospholipase C were first found, whose function can be related to the signal regulation of symbiosis. This indicates that G-proteins and phospholipases can play a key role in the development of early stages of symbiosis in peas. Further experiments are expected to show whether the beta-subunit of the G protein interacts with the receptors to Nod factors, and how this affects the further signaling. Other proteins that might be interesting were annexin D8 and D1, protein kinase interacting with calcinerin B, actin-binding protein profilin, GTP-binding protein Ran1. They may be involved in the regulation of reactions with calcium, the reorganization of the actin cytoskeleton and other important processes in plants. The study of the role of such regulatory proteins will later become the basis for understanding the complex system of signal regulation, which is activated in pea plants by interaction with nodule bacteria.https://vavilov.elpub.ru/jour/article/view/1441pea pisum sativum l.legume-rhizobial symbiosisproteomic analysisdifferential 2-d electrophoresis |
| spellingShingle | I. V. Leppyanen A. N. Kirienko A. A. Lobov E. A. Dolgikh Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria Вавиловский журнал генетики и селекции pea pisum sativum l. legume-rhizobial symbiosis proteomic analysis differential 2-d electrophoresis |
| title | Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| title_full | Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| title_fullStr | Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| title_full_unstemmed | Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| title_short | Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| title_sort | differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria |
| topic | pea pisum sativum l. legume-rhizobial symbiosis proteomic analysis differential 2-d electrophoresis |
| url | https://vavilov.elpub.ru/jour/article/view/1441 |
| work_keys_str_mv | AT ivleppyanen differentialproteomeanalysisofpearootsattheearlystagesofsymbiosiswithnodulebacteria AT ankirienko differentialproteomeanalysisofpearootsattheearlystagesofsymbiosiswithnodulebacteria AT aalobov differentialproteomeanalysisofpearootsattheearlystagesofsymbiosiswithnodulebacteria AT eadolgikh differentialproteomeanalysisofpearootsattheearlystagesofsymbiosiswithnodulebacteria |