SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS
Differentiation between adulteration and accidental meat raw material contamination in meat industry enterprises that carry out the combined processing of slaughtering products from farm animals and poultry is necessary to establish a threshold of technically non-removable impurities. Justification of...
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| Format: | Article |
| Language: | English |
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The V.M. Gorbatov All-Russian Meat Research Institute
2017-04-01
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| Series: | Теория и практика переработки мяса |
| Subjects: | |
| Online Access: | https://www.meatjournal.ru/jour/article/view/45 |
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| author | M. Yu. Minaev G. I. Solodovnikova K. A. Kurbakov |
| author_facet | M. Yu. Minaev G. I. Solodovnikova K. A. Kurbakov |
| author_sort | M. Yu. Minaev |
| collection | DOAJ |
| description | Differentiation between adulteration and accidental meat raw material contamination in meat industry enterprises that carry out the combined processing of slaughtering products from farm animals and poultry is necessary to establish a threshold of technically non-removable impurities. Justification of the thresholds, e.g. for chicken meat, requires determination of the target analytical matrix, which content in meat raw material is stable. In the Russian certified methods, the species-specific DNA matrix for chickens is a multi-copy gene of cytochrome B in mitochondrial DNA. Taking into consideration that mitochondrial DNA copy number can depend on a muscle fiber type, animal age, and other factors, the effectiveness of using multi-copy mitochondrial genes for quantifying the poultry content in meat products was justified in this study. Analysis of the samples from the pectoral and hip muscles of three chicken carcasses and one duck carcass obtained from different manufacturers showed that the poultry pectoral and hip muscles contained approximately equal amounts of mitochondrial DNA, which allows its use as a matrix to justify the level of technically non-removable chicken impurities in finished meat products. |
| format | Article |
| id | doaj-art-432c8f250dc44b08ab8e67c02ed0bf36 |
| institution | DOAJ |
| issn | 2414-438X 2414-441X |
| language | English |
| publishDate | 2017-04-01 |
| publisher | The V.M. Gorbatov All-Russian Meat Research Institute |
| record_format | Article |
| series | Теория и практика переработки мяса |
| spelling | doaj-art-432c8f250dc44b08ab8e67c02ed0bf362025-08-20T03:21:23ZengThe V.M. Gorbatov All-Russian Meat Research InstituteТеория и практика переработки мяса2414-438X2414-441X2017-04-0121273610.21323/2414-438X-2017-2-1-27-3634SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTSM. Yu. Minaev0G. I. Solodovnikova1K. A. Kurbakov2The V.M. Gorbatov All-Russian Meat Research InstituteThe V.M. Gorbatov All-Russian Meat Research InstituteThe V.M. Gorbatov All-Russian Meat Research InstituteDifferentiation between adulteration and accidental meat raw material contamination in meat industry enterprises that carry out the combined processing of slaughtering products from farm animals and poultry is necessary to establish a threshold of technically non-removable impurities. Justification of the thresholds, e.g. for chicken meat, requires determination of the target analytical matrix, which content in meat raw material is stable. In the Russian certified methods, the species-specific DNA matrix for chickens is a multi-copy gene of cytochrome B in mitochondrial DNA. Taking into consideration that mitochondrial DNA copy number can depend on a muscle fiber type, animal age, and other factors, the effectiveness of using multi-copy mitochondrial genes for quantifying the poultry content in meat products was justified in this study. Analysis of the samples from the pectoral and hip muscles of three chicken carcasses and one duck carcass obtained from different manufacturers showed that the poultry pectoral and hip muscles contained approximately equal amounts of mitochondrial DNA, which allows its use as a matrix to justify the level of technically non-removable chicken impurities in finished meat products.https://www.meatjournal.ru/jour/article/view/45real-time pcrquantitative pcrpoultry meatmitochondrial dnacytochrome bnon-removable impuritiesadulteration |
| spellingShingle | M. Yu. Minaev G. I. Solodovnikova K. A. Kurbakov SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS Теория и практика переработки мяса real-time pcr quantitative pcr poultry meat mitochondrial dna cytochrome b non-removable impurities adulteration |
| title | SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS |
| title_full | SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS |
| title_fullStr | SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS |
| title_full_unstemmed | SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS |
| title_short | SELECTION OF DNA MATRIX FOR JUSTIFICATION OF THRESHOLD FOR CONTAMINATION OF PROCESSED MEAT PRODUCTS WITH UNDECLARED POULTRY COMPONENTS |
| title_sort | selection of dna matrix for justification of threshold for contamination of processed meat products with undeclared poultry components |
| topic | real-time pcr quantitative pcr poultry meat mitochondrial dna cytochrome b non-removable impurities adulteration |
| url | https://www.meatjournal.ru/jour/article/view/45 |
| work_keys_str_mv | AT myuminaev selectionofdnamatrixforjustificationofthresholdforcontaminationofprocessedmeatproductswithundeclaredpoultrycomponents AT gisolodovnikova selectionofdnamatrixforjustificationofthresholdforcontaminationofprocessedmeatproductswithundeclaredpoultrycomponents AT kakurbakov selectionofdnamatrixforjustificationofthresholdforcontaminationofprocessedmeatproductswithundeclaredpoultrycomponents |