Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient
A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2002-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.286 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849407940345200640 |
|---|---|
| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and the centrifugation conditions (100,000-200,000 g for 2-16 h) vary with the type of cell and the requirements of the separation. The strategy is widely used to study the processing of proteins within cells. |
| format | Article |
| id | doaj-art-4321feb7cc824608b41fc36dd4e55201 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4321feb7cc824608b41fc36dd4e552012025-08-20T03:35:54ZengWileyThe Scientific World Journal1537-744X2002-01-0121435143910.1100/tsw.2002.286Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol GradientJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, USAA continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and the centrifugation conditions (100,000-200,000 g for 2-16 h) vary with the type of cell and the requirements of the separation. The strategy is widely used to study the processing of proteins within cells.http://dx.doi.org/10.1100/tsw.2002.286 |
| spellingShingle | John Graham Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient The Scientific World Journal |
| title | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
| title_full | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
| title_fullStr | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
| title_full_unstemmed | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
| title_short | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
| title_sort | fractionation of golgi endoplasmic reticulum and plasma membrane from cultured cells in a preformed continuous iodixanol gradient |
| url | http://dx.doi.org/10.1100/tsw.2002.286 |
| work_keys_str_mv | AT johngraham fractionationofgolgiendoplasmicreticulumandplasmamembranefromculturedcellsinapreformedcontinuousiodixanolgradient |