EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO
Artemisinin is the main antimalarial compound in Artemisia annua, used in the formulation of artemisinin-based combined therapies (ACT) to treat malaria. Artemisinin is largely obtained from A. annua plant but the content is very low and its production commercially is not cost effective worldwide. I...
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Alexandru Ioan Cuza University of Iasi
2024-12-01
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| Series: | Journal of Plant Development |
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| Online Access: | https://plant-journal.uaic.ro/docs/2024/6.pdf |
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| author | Lawal Gandi ABDULKADIR Adamu Aliyu ALIERO Hassan Muhammad MAISHANU Abdullahi Yahaya ABBAS |
| author_facet | Lawal Gandi ABDULKADIR Adamu Aliyu ALIERO Hassan Muhammad MAISHANU Abdullahi Yahaya ABBAS |
| author_sort | Lawal Gandi ABDULKADIR |
| collection | DOAJ |
| description | Artemisinin is the main antimalarial compound in Artemisia annua, used in the formulation of artemisinin-based combined therapies (ACT) to treat malaria. Artemisinin is largely obtained from A. annua plant but the content is very low and its production commercially is not cost effective worldwide. In view of the importance of this phytomolecule and plants being the only source of its production, this study evaluated the effect of irrigation interval on the growth and artemisinin content of A. annua. A greenhouse experiment was conducted at Botanical Garden of Usmanu Danfodiyo University Sokoto. The experiment was laid out in completely randomized design with 4 replications. The two factors examined were: (1) irrigation interval at different growth stage and (2) artemisinin content of A. annua. Irrigation interval (W) was taken at four levels (W1, W2, W4, and W6 days interval) while growth stage at three levels: early vegetative stage (EVS), mid vegetative stage (MVS) and late vegetative stage (LVD) and artemisinin were determined and quantified using High Performance Liquid Chromatography (HPLC). The calibration curve was constructed by plotting the peak area against the concentration by the external standard method on five concentration levels of artemisinin standard (5, 10, 15, 20, and 25 µg/ml), with three injections per level. Linear regression was used to establish the calibration curve. Results were calculated using the peak areas with determination coefficient (R2) of 0.951. Results revealed that W2 days irrigation interval had significantly (p<0.05) affected fresh weight, dry weight, stem diameter and root length during the EVS. At MVS, irrigation interval had no significant (P>0.05) effect on plant height and number of branches but significantly reduces stem diameter. During the late vegetative stage (LVD), irrigation intervals have no significant effect on all parameters evaluated. Artemisinin content was not significantly (P>0.05) affected by irrigation intervals during the EVS but significantly affected at mid and late vegetative stage and our result demonstrated that prolong mild irrigation interval (W2) could significantly enhanced artemisinin content in A. annua. |
| format | Article |
| id | doaj-art-4321e05ba2b740d39e6807ef269df54d |
| institution | Kabale University |
| issn | 2065-3158 2066-9917 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Alexandru Ioan Cuza University of Iasi |
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| series | Journal of Plant Development |
| spelling | doaj-art-4321e05ba2b740d39e6807ef269df54d2025-01-17T12:07:58ZengAlexandru Ioan Cuza University of IasiJournal of Plant Development2065-31582066-99172024-12-01317582https://doi.org/10.47743/jpd.2024.31.1.964EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTOLawal Gandi ABDULKADIR0Adamu Aliyu ALIERO1Hassan Muhammad MAISHANU2Abdullahi Yahaya ABBAS3Department of Plant Science, Usmanu Danfodiyo University, Sokoto – NigeriaDepartment of Plant Science, Usmanu Danfodiyo University, Sokoto – NigeriaDepartment of Plant Science, Usmanu Danfodiyo University, Sokoto – NigeriaDepartment of Environmental Biology, Shehu Shagari University of Education, Farfaru, Sokoto – NigeriaArtemisinin is the main antimalarial compound in Artemisia annua, used in the formulation of artemisinin-based combined therapies (ACT) to treat malaria. Artemisinin is largely obtained from A. annua plant but the content is very low and its production commercially is not cost effective worldwide. In view of the importance of this phytomolecule and plants being the only source of its production, this study evaluated the effect of irrigation interval on the growth and artemisinin content of A. annua. A greenhouse experiment was conducted at Botanical Garden of Usmanu Danfodiyo University Sokoto. The experiment was laid out in completely randomized design with 4 replications. The two factors examined were: (1) irrigation interval at different growth stage and (2) artemisinin content of A. annua. Irrigation interval (W) was taken at four levels (W1, W2, W4, and W6 days interval) while growth stage at three levels: early vegetative stage (EVS), mid vegetative stage (MVS) and late vegetative stage (LVD) and artemisinin were determined and quantified using High Performance Liquid Chromatography (HPLC). The calibration curve was constructed by plotting the peak area against the concentration by the external standard method on five concentration levels of artemisinin standard (5, 10, 15, 20, and 25 µg/ml), with three injections per level. Linear regression was used to establish the calibration curve. Results were calculated using the peak areas with determination coefficient (R2) of 0.951. Results revealed that W2 days irrigation interval had significantly (p<0.05) affected fresh weight, dry weight, stem diameter and root length during the EVS. At MVS, irrigation interval had no significant (P>0.05) effect on plant height and number of branches but significantly reduces stem diameter. During the late vegetative stage (LVD), irrigation intervals have no significant effect on all parameters evaluated. Artemisinin content was not significantly (P>0.05) affected by irrigation intervals during the EVS but significantly affected at mid and late vegetative stage and our result demonstrated that prolong mild irrigation interval (W2) could significantly enhanced artemisinin content in A. annua.https://plant-journal.uaic.ro/docs/2024/6.pdfartemisininearly vegetative stage (evs)mid vegetative stage (mvs)late vegetative stage (lvd)high performance liquid chromatography (hplc) |
| spellingShingle | Lawal Gandi ABDULKADIR Adamu Aliyu ALIERO Hassan Muhammad MAISHANU Abdullahi Yahaya ABBAS EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO Journal of Plant Development artemisinin early vegetative stage (evs) mid vegetative stage (mvs) late vegetative stage (lvd) high performance liquid chromatography (hplc) |
| title | EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO |
| title_full | EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO |
| title_fullStr | EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO |
| title_full_unstemmed | EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO |
| title_short | EFFECT OF IRRIGATION INTERVAL ON GROWTH AND ARTEMISININ CONTENT OF WORMWOOD (Artemisia annua L.) CHEN YOUNG VARIETY IN SOKOTO |
| title_sort | effect of irrigation interval on growth and artemisinin content of wormwood artemisia annua l chen young variety in sokoto |
| topic | artemisinin early vegetative stage (evs) mid vegetative stage (mvs) late vegetative stage (lvd) high performance liquid chromatography (hplc) |
| url | https://plant-journal.uaic.ro/docs/2024/6.pdf |
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