Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023
<i>Salmonella</i> is a major threat to both human and animal health. However, the diversity and antibiotic resistance of animal-derived <i>Salmonella</i> and their association with human infections remain largely unexplored. In this study, <i>Salmonella</i> strain...
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2024-12-01
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| author | Zhiyuan Lu Yue Zheng Shaopeng Wu Xiaoyue Lin Huiling Ma Xiaofei Xu Shumin Chen Jiaqi Huang Zheng Gao Guisheng Wang Shuhong Sun |
| author_facet | Zhiyuan Lu Yue Zheng Shaopeng Wu Xiaoyue Lin Huiling Ma Xiaofei Xu Shumin Chen Jiaqi Huang Zheng Gao Guisheng Wang Shuhong Sun |
| author_sort | Zhiyuan Lu |
| collection | DOAJ |
| description | <i>Salmonella</i> is a major threat to both human and animal health. However, the diversity and antibiotic resistance of animal-derived <i>Salmonella</i> and their association with human infections remain largely unexplored. In this study, <i>Salmonella</i> strains were isolated, identified, and sequenced from dead embryos and cloacal swab samples obtained from 278 large-scale duck farms in 11 cities in Shandong Province. The results show that a total of 57 <i>Salmonella</i> strains were isolated, with the dominant sequence types (ST) being ST17 (15/57) and ST19 (9/57), while the dominant serotypes were <i>S. Indiana</i> (15/57) and <i>S. Typhimurium</i> (11/57). Furthermore, genomic analysis has revealed the presence of prevalent antibiotic resistance genes (ARGs), which are often associated with co-transfer mechanisms. Over 52.63% of the strains were observed to carry two or more ARGs, especially one <i>Salmonella</i> strain that carried twenty-eight distinct ARGs. Furthermore, core genome multilocus sequence typing analysis (cgMLST) indicated that the 57 <i>Salmonella</i> strains may have a close relationship, which could be clonally transmitted among different cities. The results demonstrated a close relationship between the <i>Salmonella</i> strains identified in diverse geographical regions, suggesting that these strains may have been widely disseminated through clonal transmission. The mutation analysis reveals significant mutations at <i>parC</i> (T57S), <i>gyrA</i> (S83F), <i>parC</i> (S80R), <i>gyrA</i> (D87N), and <i>gyrA</i> (S83Y). These findings emphasize the necessity for monitoring and controlling <i>Salmonella</i> infections in animals, as they may serve as a reservoir for ARGs with the potential to affect human health or even be the source of pathogens that infect humans. |
| format | Article |
| id | doaj-art-430ed73125fb4f27964877b63a84ed02 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microorganisms |
| spelling | doaj-art-430ed73125fb4f27964877b63a84ed022025-08-20T02:43:46ZengMDPI AGMicroorganisms2076-26072024-12-011212261910.3390/microorganisms12122619Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023Zhiyuan Lu0Yue Zheng1Shaopeng Wu2Xiaoyue Lin3Huiling Ma4Xiaofei Xu5Shumin Chen6Jiaqi Huang7Zheng Gao8Guisheng Wang9Shuhong Sun10Shandong Provincial Key Laboratory of Zoonoses, College of Animal Medicine, Shandong Agricultural University, Tai’an 271002, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271002, ChinaShandong Provincial Key Laboratory of Zoonoses, College of Animal Medicine, Shandong Agricultural University, Tai’an 271002, ChinaShandong Provincial Center for Animal Disease Control, Jinan 250100, ChinaShandong Provincial Center for Animal Disease Control, Jinan 250100, ChinaShandong Provincial Center for Animal Disease Control, Jinan 250100, ChinaShandong Provincial Center for Animal Disease Control, Jinan 250100, ChinaShandong Provincial Key Laboratory of Zoonoses, College of Animal Medicine, Shandong Agricultural University, Tai’an 271002, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271002, ChinaShandong Provincial Center for Animal Disease Control, Jinan 250100, ChinaShandong Provincial Key Laboratory of Zoonoses, College of Animal Medicine, Shandong Agricultural University, Tai’an 271002, China<i>Salmonella</i> is a major threat to both human and animal health. However, the diversity and antibiotic resistance of animal-derived <i>Salmonella</i> and their association with human infections remain largely unexplored. In this study, <i>Salmonella</i> strains were isolated, identified, and sequenced from dead embryos and cloacal swab samples obtained from 278 large-scale duck farms in 11 cities in Shandong Province. The results show that a total of 57 <i>Salmonella</i> strains were isolated, with the dominant sequence types (ST) being ST17 (15/57) and ST19 (9/57), while the dominant serotypes were <i>S. Indiana</i> (15/57) and <i>S. Typhimurium</i> (11/57). Furthermore, genomic analysis has revealed the presence of prevalent antibiotic resistance genes (ARGs), which are often associated with co-transfer mechanisms. Over 52.63% of the strains were observed to carry two or more ARGs, especially one <i>Salmonella</i> strain that carried twenty-eight distinct ARGs. Furthermore, core genome multilocus sequence typing analysis (cgMLST) indicated that the 57 <i>Salmonella</i> strains may have a close relationship, which could be clonally transmitted among different cities. The results demonstrated a close relationship between the <i>Salmonella</i> strains identified in diverse geographical regions, suggesting that these strains may have been widely disseminated through clonal transmission. The mutation analysis reveals significant mutations at <i>parC</i> (T57S), <i>gyrA</i> (S83F), <i>parC</i> (S80R), <i>gyrA</i> (D87N), and <i>gyrA</i> (S83Y). These findings emphasize the necessity for monitoring and controlling <i>Salmonella</i> infections in animals, as they may serve as a reservoir for ARGs with the potential to affect human health or even be the source of pathogens that infect humans.https://www.mdpi.com/2076-2607/12/12/2619<i>Salmonella</i>whole genome sequencingsequence typesduckantibiotic resistance genesmutation |
| spellingShingle | Zhiyuan Lu Yue Zheng Shaopeng Wu Xiaoyue Lin Huiling Ma Xiaofei Xu Shumin Chen Jiaqi Huang Zheng Gao Guisheng Wang Shuhong Sun Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 Microorganisms <i>Salmonella</i> whole genome sequencing sequence types duck antibiotic resistance genes mutation |
| title | Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 |
| title_full | Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 |
| title_fullStr | Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 |
| title_full_unstemmed | Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 |
| title_short | Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023 |
| title_sort | antimicrobial resistance genes and clonal relationships of duck derived i salmonella i in shandong province china in 2023 |
| topic | <i>Salmonella</i> whole genome sequencing sequence types duck antibiotic resistance genes mutation |
| url | https://www.mdpi.com/2076-2607/12/12/2619 |
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