Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon
Sulfated glycosaminoglycans (GAGs) or chondroitin sulfates were obtained from the cartilage tissue of skate (indefinite species) and sturgeon (kaluga) under the processes of hydrothermal treatment of raw materials at 50 оC, then hydrolysis with proteolytic enzyme in 4 hours under temperature 45 оC (...
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| Format: | Article |
| Language: | Russian |
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Transactions of the Pacific Research Institute of Fisheries and Oceanography
2023-01-01
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| Series: | Известия ТИНРО |
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| Online Access: | https://izvestiya.tinro-center.ru/jour/article/view/791 |
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| author | A. I. Chepkasova T. N. Slutskaya E. P. Karaulova |
| author_facet | A. I. Chepkasova T. N. Slutskaya E. P. Karaulova |
| author_sort | A. I. Chepkasova |
| collection | DOAJ |
| description | Sulfated glycosaminoglycans (GAGs) or chondroitin sulfates were obtained from the cartilage tissue of skate (indefinite species) and sturgeon (kaluga) under the processes of hydrothermal treatment of raw materials at 50 оC, then hydrolysis with proteolytic enzyme in 4 hours under temperature 45 оC (hydromodulus 1 : 1), then sequential precipitation of GAGs in 2–3 volumes of 96о ethyl alcohol and salting out with 2 % sodium chloride solution. The content of hexosamines and sulfated GAGs was determined in the extracted samples. GAGs of both skate and sturgeon have a high content of sulfated forms, comparable to commercial preparations, that opens up prospects for development of technology for production of monocomponent biologically active additives of chondroprotective action from the wastes of skates and sturgeons processing. |
| format | Article |
| id | doaj-art-430c539ec0204334bc459d0d6ee96d90 |
| institution | DOAJ |
| issn | 1606-9919 2658-5510 |
| language | Russian |
| publishDate | 2023-01-01 |
| publisher | Transactions of the Pacific Research Institute of Fisheries and Oceanography |
| record_format | Article |
| series | Известия ТИНРО |
| spelling | doaj-art-430c539ec0204334bc459d0d6ee96d902025-08-20T03:19:02ZrusTransactions of the Pacific Research Institute of Fisheries and OceanographyИзвестия ТИНРО1606-99192658-55102023-01-01202494695610.26428/1606-9919-2022-202-946-956698Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeonA. I. Chepkasova0T. N. Slutskaya1E. P. Karaulova2Pacific branch of VNIRO (TINRO)Pacific branch of VNIRO (TINRO)Pacific branch of VNIRO (TINRO)Sulfated glycosaminoglycans (GAGs) or chondroitin sulfates were obtained from the cartilage tissue of skate (indefinite species) and sturgeon (kaluga) under the processes of hydrothermal treatment of raw materials at 50 оC, then hydrolysis with proteolytic enzyme in 4 hours under temperature 45 оC (hydromodulus 1 : 1), then sequential precipitation of GAGs in 2–3 volumes of 96о ethyl alcohol and salting out with 2 % sodium chloride solution. The content of hexosamines and sulfated GAGs was determined in the extracted samples. GAGs of both skate and sturgeon have a high content of sulfated forms, comparable to commercial preparations, that opens up prospects for development of technology for production of monocomponent biologically active additives of chondroprotective action from the wastes of skates and sturgeons processing.https://izvestiya.tinro-center.ru/jour/article/view/791skatekalugacartilage tissuechondroitin sulfateglycosaminoglycanssulfated glycosaminoglycanshexosamineschondroprotector |
| spellingShingle | A. I. Chepkasova T. N. Slutskaya E. P. Karaulova Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon Известия ТИНРО skate kaluga cartilage tissue chondroitin sulfate glycosaminoglycans sulfated glycosaminoglycans hexosamines chondroprotector |
| title | Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| title_full | Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| title_fullStr | Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| title_full_unstemmed | Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| title_short | Characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| title_sort | characteristics of sulfated glycosaminoglycans in the cartilage tissue of skate and sturgeon |
| topic | skate kaluga cartilage tissue chondroitin sulfate glycosaminoglycans sulfated glycosaminoglycans hexosamines chondroprotector |
| url | https://izvestiya.tinro-center.ru/jour/article/view/791 |
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